白桦木质部蛋白提取方法的建立1）

以白桦（ Betula platyphylla Suks．）木质部为试验材料,分别采用TCA—丙酮提取、酚提取和试剂盒提取3种方法提取白桦木质部总蛋白。利用试剂盒定量和聚丙烯酰胺凝胶电泳、双向电泳技术比较3种提取方法的蛋白质提取效率、提取质量。同时对酚提取法进行改良,建立适用于白桦木质部蛋白的提取方法。研究结果表明,改良的酚提取法提取的白桦木质部蛋白提取量最大,电泳条带清晰,丰富,质量较高。2－DE电泳得到的蛋白凝胶背景干净,蛋白质点清晰。因此,改良的酚提取法是较适合于白桦木质部蛋白的提取方法。     

基于关键帧提取技术的花开过程视频监测系统开发及试验

为克服传统花开过程监测中视频数据冗余、信息量大的缺点,该文设计了一种基于关键帧提取技术的花开过程视频监测系统。系统对花开过程原始图像采集后,采用基于光流法和熵统计算法实现对花开过程原始图像的关键帧提取,选择关键帧数模式或方向信息熵阈值模式,进行相关参数设置,最终合成出表征花开过程的关键帧视频。该文以百合花开放过程为例实现了基于关键帧提取技术的视频监测。试验结果证明,在该试验的条件下经关键帧提取合成的花开过程的视频数据量减少达84.6%以上,播放时间减少为原始视频播放时间的15.4%以下。视频保留了花开过程细节信息,整段视频播放自然流畅,可为从事植物、花卉研究的相关人员提供一个省时、方便的研究花开过程的监测平台。     

基于区域气球力Snake模型的运动目标快速跟踪方法

提出了一种包含区域信息的Snake模型用于运动目标检测与跟踪。在通常情况下,基于区域信息的跟踪方法对背景光线的微小变化、位置的微小移动较为敏感,而基于边缘信息的跟踪方法则难以对边缘模糊的图像取得满意的跟踪效果。在算法中同时引入这两种信息,边缘信息使得算法快速而鲁棒性好,区域信息可以对边缘模糊的图像取得正确的跟踪效果。使用双差分图像设计了自动初始化的方法来实现视频的自动跟踪。同时,对目标的下一步运动位置增加了一个预测环节来加快主动轮廓模型的收敛速度。该算法的每帧计算时间一般小于0.1S,能应用于实时系统。     

基于GIS的森林火灾自动定位系统设计与开发

根据森林防火管理的实际需求,以嘹望塔视频监控设备为硬件基础,提出了双点定位方法与单点定位方法相结合的综合方案,综合运用组件GIS、ArcSDE数据库引擎、.NET等多种先进软件技术设计开发了鹫峰国家森林公园森林火灾定位系统,解决了目前森林防火基层管理亟待解决的着火点现场定位问题.     

黄瓜杂交二代抗黄瓜白粉病的蛋白质组学初步分析

通过分组分离法建立F₂代抗感池, 利用2-DE差异显示和质谱分析研究了抗病池和感病池的差异蛋白质组。抗感池经双向电泳检测到近500个蛋白点, 质谱分析鉴定出9种抗感池表达差异蛋白点,分别为抗病基因蛋白( SSP3110) , 14-3-3脑蛋白家族蛋白( SSP1411) , 粪卟啉原氧化酶Ⅲ ( SSP5513) , 碳酸酐酶( SSP5412 ) , α - 半乳糖苷酶( SSP5710 ) , 推定的蛋白( SSP6002 ) , 17.8 kDa 的热激蛋白( SSP0710) , 假想蛋白( SSP3111) , ( S) - 2 - 羟酸氧化酶( SSP4610) 。其中有3种蛋白的性质及生物学功能未知, 其余6种蛋白分别与光合作用、呼吸作用、抗病反应及信号转导等有密切关系。这些蛋白都可能是与抗性有关或与发育相关的蛋白网络中的一部分, 它们在黄瓜抗病反应中起着不同的作用。     

From Framework to Forest Activities: A Comparative Analysis of Different Ownership Types in Two Adjacent Regions

The paper examines the interactions between the demands of society, the restrictions due to recreational and protective functions of forests and the intensity and kind of forest management in the various ownership classes in Switzerland (CH) and Baden–Württemberg (BW) in south–west Germany. A survey was carried out among owners of communal forests, private forest landholders owning more than 200 ha of forest land and small-scale forest enterprises with up to 200 ha of forest land. The survey adopted a two-dimensional approach, using regions and comparable ownership classes in order to identify basic differences and underlying key drivers. It became obvious that there are relevant distinctions between the two countries. The paper highlights the present use of forests, the restrictions perceived by their owners, and the underlying management strategies in terms of structure of the forests, tree species composition and thinning activities. In BW the financial function is the dominant motivation for the majority of the private owners. The communities emphasize both timber and non-timber functions of their forests. Except for the protection function (avalanches, rockslides) the utilisation and importance of the forest in CH is lower than in BW. Profitability of forests continues to be a key-driver of intensity of forest management activities, but there is less influence on the choice of basic silvicultural strategies. The results of this comparative survey are placed in the context of a forest policy framework and the medium-term financial situation in the two countries.

基于MPEG-4的网络视频监控系统拥塞控制方法研究

有效可靠的的传输,是视频监控系统的一个重要研究课题。分析了基于MPEG-4的网络视频监控系统可以采用的拥塞控制方法,提出了一种基于RTP协议,从分组丢失率、时延、时延抖动三方面判断网络拥塞,综合考虑发送、接收和中间节点三方面影响的拥塞控制方法。     

The use of accelerometer to measure sleeping posture of beef cows

Sleep is one of the essential behaviors for mammals. The aims of this study were to validate the use of accelerometer for measuring sleeping posture of cattle. Duration of sleeping posture of seven Japanese Black cows from 19.00 to 07.00 hours was measured by both accelerometer and video, and a total of 67 accelerometer and video measurement sets were collected. We calculated Cohen's κ coefficient between accelerometer and video measurements and 91.5% of the κ‐values were >0.80. Intra‐ and inter‐observer coefficient of variance showed that specific acceleration waveform patterns of sleeping posture could be easily and accurately detected by independent observers. There were no significant differences in the frequency of sleeping posture occurrences between accelerometer and video measurements. We compared averaged sleeping posture bout, and the total sleeping posture time between accelerometer and video measurements using regression. In each trait, the slope was close to 1 and the intercept was not different from 0, which showed a strong agreement between accelerometer and video measurements. This shows that an accelerometer could accurately detect sleeping postures of cattle. We conclude that adequate measurements of sleeping postures can be made using an accelerometer.     

基于小波域分水岭变换的视频对象提取算法

提出了一种基于时、空分割融合的视频对象分割算法．时间分割采用变化检测，其关键的阈值选取通过直方图分析得到．空间分割采用小渡域的分水岭变换算法．时、空分割融合采用基于区域的双向投影策略．实验结果表明，本算法可比COST211AM参考分割取得更好的分割结果．