番茄灰霉病分子诊断方法研究

为提供番茄灰霉病的早期诊断技术,应用其病原菌灰葡萄孢种特异片段P729分子标志,建立了PCR检测方法,并进行了实验室和大田现场标本的评价。结果显示番茄灰霉病的分子诊断方法在实验室具有特异、稳定和可靠的特点。比较6种抽提基因组DNA的方法,方法B效果理想。应用建立的分子诊断方法可从0.2μg灰葡萄孢菌丝中获得阳性扩增,具有相当高的灵敏性;大田样本的检测结果说明该方法可用于番茄灰霉病的早期诊断。     

海岛棉植株测试推荐施肥技术初步研究

通过膜下滴灌条件下海岛棉多水平氮肥试验,分析了海岛棉倒四叶叶柄硝酸盐浓度、生物量、植株全氮和产量的关系,结果表明:海岛棉倒四叶叶柄硝酸盐浓度能够快速、灵敏地反映作物的氮营养状况,可用作海岛棉的氮素营养诊断及氮肥需要量的测算指标.用一元二次模型模拟氮肥与产量之间的关系,得到经济氮量为206 kg/hm2,相应产量为1 182 kg/hm2,棉株花期叶柄硝酸盐浓度与施氮量和产量之间有显著的相关性,以此为依据建立海岛棉植株硝酸盐诊断的氮肥推荐模型,并计算出花期时不同硝酸盐测试值所对应的氮肥追肥用量.     

数字图像技术在草莓氮素营养诊断中的应用研究

为了探讨利用数字图像处理技术进行草莓氮素营养诊断的可行性,通过6个水平的氮肥田间试验,采用数码相机获取草莓冠层图像,分析了不同供氮水平下草莓冠层图像色彩参数与施氮量、土壤无机氮和植株氮素营养指标之间的关系.研究表明:数字图像技术应用于监测草莓的氮素供应状况是可行的.其中,不同氮素处理下G/B值与G/(R+G+B)值的决定系数较高,G/(R+G+B)值与土壤无机氮、叶片硝酸盐及植株全氮之间的决定系数最高,利用G/(R+G+B)值的范围得出草莓开花期与结果期的氮肥推荐用量,从而反馈草莓氮素营养状况,进行氮素营养诊断.     

Design and validation of a RT‐qPCR procedure for diagnosis and quantification of most types of infectious pancreatic necrosis virus using a single pair of degenerated primers

Infectious pancreatic necrosis virus (IPNV) is an important virus which affects the salmonid aquaculture industry worldwide; therefore, it is important to develop rapid and reliable methods of diagnosis to detect the disease at early stages. Nowadays, RT‐qPCR is replacing other methods because it provides additional information on the viral load, which is important to have a better understanding of the virus replication level and of the stage of the infection and its risk level. The main problem stems from the high diversity of this virus, which can compromise the reliability of the diagnosis. In this study, we have designed an RT‐qPCR procedure for diagnosis and quantification of IPNV based on a single pair of primers targeted to segment B. The procedure has been validated, in vitro and in vivo, testing two different types of standards against seven reference strains and 23 field isolates from different types. The procedure is reliable for the detection of any type, with a detection limit of 31 TCID₅₀ mL^?1, 50 pfu mL^?1 or 66 RNA copies mL^?1, depending on the standard. All the standard curves showed high reliability (R² > 0.95). The results support the high reliability of this new procedure for the diagnosis and quantification of IPNV.     

机械设备故障诊断用便携式数据采集分析系统

设计了一套应用于机械设备故障诊断的便携式数据采集分析系统。系统整体采用主从结构:上位机软件基于Labview,主要实现路径、数据、通讯等的管理以及数据分析;下位机实现采集、分析。下位机的硬件层核心采用片上系统(SoC)芯片;操作系统层移植了自行研发的实时操作系统(RTOS):TDNC-OS,给出了任务划分;应用软件层引入了缓冲区操作机制;通讯层主要由UART模块组成。实例表明系统具有很好的实时性和可靠性。     

一起东佛里生奶绵羊附红细胞体病流行病学调查、诊疗报告

为探究附红细胞体在内蒙古呼和浩特市托克托县地区的流行情况,接诊呼和浩特托县地区某东佛里生奶绵羊养殖场病例,该场近一周多圈舍出现产奶羊只体温升高、精神沉郁、瘫软、食欲减退或废绝等症状,有出现症状1 ~ 2 d迅速死亡病例,也有使用抗生素硫酸庆大霉素-青霉素联用结合补液治疗1周无好转或死亡病例,死亡总数超8只,仍有10余只相似症状正在接受治疗。经现场剖检、圈舍环境调查、样品实验室检测,结合临床诊疗,确诊此次疫情为奶绵羊附红细胞体病,且周边羊养殖场（户）羊只也存在附红细胞体感染。说明附红细胞体在托克托县呈地方性流行。     

汽车搭铁线电路故障的诊断与处理

汽车上将蓄电池负极与车身的金属部分相连接的导线通常称为搭铁线。搭铁线虽小,在汽车电路中却起着重要的作用。着重介绍了汽车搭铁线的类型、作用、故障诊断,为正确检修提供参考。     

A rapid and easy method of detecting levels of gene expression in intact tobacco plants using an Agrobacterium-mediated transient assay: Toward development of nutritional diagnosis based on gene expression

For the use of gene expression for nutritional diagnosis, a rapid and easy method of detecting levels of gene expression was proposed. Agrobacterium tumefaciens carrying the GUS reporter gene was injected into intact tobacco leaves with a plastic syringe, and transient GUS expression was determined. GUS activities were reproducible in young leaves.     

Technologies for the diagnosis and remediation of Fe deficiency

The objective of this paper is to review the developments in the last few years in two important issues related to Fe deficiency in plants. First, the current knowledge on the possible ways to carry out the diagnosis and prognosis of Fe deficiency in plants is discussed. This includes discussion on the best ways to carry out a meaningful analysis of Fe-containing compounds in different plant parts. We will also discuss other measurement techniques that can permit to assess the Fe nutritional status in plants, including leaf chlorophyll concentrations and others. Second, the new developments in management techniques to control and remediate iron deficiency are discussed. This includes possible improved ways to supply Fe compounds available to plants, both to the soil and to the irrigation water. We also discuss possible ways to supply directly the plant with Fe containing compounds, either to the foliage or to the stem. A particular emphasis is given throughout the paper to fruit tree crops growing in Mediterranean areas.     

便携式作物生长监测诊断仪的设计与试验

为了解决当前作物生长信息实时、快速、无损测量手段瓶颈问题,研发了一种基于多光谱传感器的便携式作物生长监测诊断仪。该仪器由多光谱传感器系统、处理器系统及附属机构组成,能实时无损地获取作物叶层氮含量、叶层氮积累量、叶面积指数、生物量等主要生长指标。对水稻的试验结果表明,便携式作物生长监测诊断仪测得的DVI值与水稻叶层氮含量、氮积累量、叶面积指数、叶干重的决定系数R2分别为0.711,0.8231,0.7698,0.7212。该仪器结构简单,集成度高,性价比好,携带方便,易于田间操作。