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Tillage changes soil environmental conditions and controls the distribution of residues in the soil, both actions that affect the production and emission of soil biogenic gases (CO2, N2O, and CH4). The objective of this study was to determine how tillage-induced environmental conditions and substrate quality affect the mineralization rate of easily metabolizable compounds and the subsequent production of these gases. Carbon compounds, with and without nitrogen, were applied to soil cropped to maize under tilled and no-till systems. Following substrate application in the spring and summer, biogenic gases were measured periodically at the soil surface (flux) and within the profile (concentration) at 10-, 20-, and 30-cm depths (i.e., within, at the bottom of, and below the plough layer). Strong CO2 and N2O responses to sucrose and glycine in both the field and the laboratory indicate that the soil was C- and N-limited. Surface fluxes of CO2 and N2O were greater in soils amended with glycine than with sucrose and were greater in tilled than no-till soils. Transient emission of CH4 following the addition of glycine was observed and could be attributed to inhibition of N mineralization and nitrification processes on CH4 oxidation. Laboratory and field measurements indicated that the larger substrate-induced CO2 emission from the tilled soils could not be attributed to differences in the total biomass or the basal respiratory activity of the soils. Thus, there appears to be no underlying difference in the functional capacity of the microbial communities under different tillage regimes. Comparison of gas profiles indicates relative accumulation of CO2 at depth in soils under no-till, as well as greater decline in profile CO2 content with time in the tilled compared to the no-till soil. These results support the conclusion that greater CO2 efflux from the tilled soils resulted from more rapid gas diffusion through the profile. Hence, the observed differences in gas fluxes between tilled and no-till soils can be attributed to differences in physical environment. 相似文献
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对金银花多酚氧化酶底物特异性和不同抑制剂的抑制效应进行了动力学分析,结果表明:金银花多酚氧化酶的最适作用底物为绿原酸,Km值为0.0059 mmol/L。维生素C、4-己基间苯二酚、L-半胱氨酸对多酚氧化酶的抑制均属可逆抑制,其抑制类型分别属于混合性、竞争性、反竞争性,对游离酶的抑制常数KI分别为1.620、4.587、0 mmol/L,对酶-底物络合物的抑制常数KIS分别为1.995、0、3.780 mmol/L;柠檬酸的抑制效应属不可逆抑制。维生素C、4-己基间苯二酚、L-半胱氨酸、柠檬酸对金银花多酚氧化酶半抑制浓度分别为0.062、0.053、0.140、0.048 mmol/L。 相似文献
15.
外源微生物对苦参基质化发酵腐熟效果的影响 总被引:2,自引:0,他引:2
为探讨外源微生物对苦参枝屑基质化发酵堆体腐熟效果的影响,采用随机区组设计,以干燥鸡粪为氮源,进行接种外源微生物对苦参枝屑基质化发酵过程中发酵性能参数的影响试验。结果表明:接种粗纤维降解菌和纤维素类酶制剂堆体升温速度显著快于对照,高温持续时间较长(高于50℃,均达到5 d),苦参堆体腐熟时间缩短;至堆体结束后接种粗纤维降解菌和纤维素类酶制剂堆体TOC分别下降了8.52%和8.01%,碳氮比分别降低5.35%和5.00%,纤维素质量分数分别下降了3.31%和3.29%,半纤维素降解率分别下降了2.89%和2.93%,木质素分别下降了2.01%和1.98,总氮分别增加了18.2%和16.1%,总磷分别增加了19.0%和18.5%,总钾分别增加了48.1%和49.1%,加速了苦参基质有机质的分解和纤维素降解,提高了堆肥腐熟进程中的总氮、总磷和总钾含量,保证了腐熟后的肥力;接种粗纤维降解菌和纤维素类酶制剂堆体容重分别增加了6.10%和9.20%,总孔隙度分别增加了9.28%和9.90%,持水孔隙度分别增加了4.83%和6.09%,腐熟后的各项理化指标均符合理想基质的要求;小白菜和黄瓜种子发芽指数均达到85%以上,有效消除了苦参腐解产物的毒害作用。接种粗纤维降解菌和纤维素类酶制剂堆体的发酵性能参数间无显著差异,综合基质发酵温度、腐熟周期及基质保护作物根系生长及固定植株的功能对基质各项理化性质的要求,接种粗纤维降解菌和纤维素类酶制剂对苦参基质堆体基质化促进效果较优。 相似文献
16.
Khalid S. Khan Andreas Gattinger Franz Buegger Michael Schloter Rainer Georg Joergensen 《Soil biology & biochemistry》2008,40(5):1217-1224
An incubation experiment was carried out to investigate whether salinity at high pH has negative effects on microbial substrate use, i.e. the mineralization of the amendment to CO2 and inorganic N and the incorporation of amendment C into microbial biomass C. In order to exploit natural differences in the 13C/12C ratio, substrate from two C4 plants, i.e. highly decomposed and N-rich sugarcane filter cake and less decomposed N-poor maize leaf straw, were added to two alkaline Pakistani soils differing in salinity, which had previously been cultivated with C3 plants. In soil 1, the additional CO2 evolution was equivalent to 65% of the added amount in the maize straw treatment and to 35% in the filter cake treatment. In the more saline soil 2, the respective figures were 56% and 32%. The maize straw amendment led to an identical immobilization of approximately 48 μg N g−1 soil over the 56-day incubation in both soils compared with the control soils. In the filter cake treatment, the amount of inorganic N immobilized was 8.5 μg N g−1 higher in soil 1 than in soil 2 compared with the control soils. In the control treatment, the content of microbial biomass C3-C in soil 1 was twice that in soil 2 throughout the incubation. This fraction declined by about 30% during the incubation in both soils. The two amendments replaced initially similar absolute amounts of the autochthonous microbial biomass C, i.e. 50% of the original microbial biomass C in soil 1 and almost 90% in soil 2. The highest contents of microbial biomass C4-C were equivalent to 7% (filter cake) and 11% (maize straw) of the added C. In soil 2, the corresponding values were 14% lower. Increasing salinity had no direct negative effects on microbial substrate use in the present two soils. Consequently, the differences in soil microbial biomass contents are most likely caused indirectly by salinity-induced reduction in plant growth rather than directly by negative effects of salinity on soil microorganisms. 相似文献
17.
Growing media (substrate) is a fundamental part of a green roof, providing water, nutrients and support to plants. However, little research has reviewed how it affects plant performances in different climatic regions. This study aims to analyse published research on green roof growing medium across world’s climate zones. Findings are structured according to Köppen–Geiger climate classification, aiming to investigate the prevalence of research conducted in different climate zones. Results from full-scale studies and laboratory or greenhouse experiments were reviewed. The later were included as they provide systematic knowledge on the effect of individual factors on system performances although cannot provide climate specific information. Studies discussed effects of major substrate components and depths on plant survival and establishment using standard test procedures. Results showed that most research in the subject were in temperate (group C climate classification), continental (group D) and dry climates (group B), respectively. Considerable number of investigations was conducted in controlled laboratory or greenhouse environments. Based on the results, future green roof research and guidelines should consider climate specifications of the region in designing growing medium, depths and attribute of green roof substrates in order to ensure enhanced plant performance. Especially, for more fragile but less investigated dry climate, considerations should be made to tackle heat fluctuations and drought stress by enhancing water holding capacity and thermal isolation of the substrate. To move forward, sustainable building solutions as a part of future urban forms, climate-adaptive green roof systems should be included into future research, practice and guidelines. 相似文献
18.
为了研究基质还田对大田土壤和辣椒生长生产的影响,以日光温室连续栽培蔬菜6年的废弃基质还施露地种植辣椒,测定了不同施用量(0,45,90,135 m~3/hm~2)的土壤理化性质及辣椒生理特性和产量。结果表明:基质还田能够减少土壤pH变化,减小土壤容重、略微增加土壤孔隙度、保持土壤气水比,改善了土壤的理化性状,促进辣椒植株的生长且利于其干物质积累,其中株高、茎粗及干物质积累量均以T2最好。提升了辣椒叶片光合荧光的表现,T2的净光合速率、蒸腾速率、气孔导度、Fv/Fm、ΦPSⅡ均为最高,T1的q P最高,NPQ为T3最低。提高了辣椒果实的品质及产量,T2产量达67.04 t/hm~2,可溶性糖含量以T2最高,为2.21%,Vc含量T3最高,为1.97 mg/g,T1的可溶性蛋白含量最高、硝酸盐含量最低,分别为2.07,204.93 mg/kg。而与此同时,较大的还田施用量(135 m~3/hm~2)在辣椒生长的中前期造成了一定的毒害现象,在生育后期此现象消失,最终各项指标全面优于未还田处理。废弃基质露地还田这样的重复利用方式切实有效,(在种植同科作物的情况下)还田施用量宜选择90 m~3/hm~2。 相似文献
19.
不同基质配比对黄瓜幼苗生长的影响 总被引:1,自引:0,他引:1
为提高农业废弃物利用率,减少环境污染,以黄瓜品种津绿3号为试验材料,研究牛粪、花生壳、草炭、蛭石、珍珠岩等不同基质成分配比对黄瓜幼苗生长及光合作用的影响。结果表明:不同基质配比对黄瓜的生长有显著影响。其中C处理(花生壳︰蛭石︰珍珠岩体积比7︰3︰3)效果最好,育苗40 d后,黄瓜幼苗叶片大而肥厚,叶色浓绿,株高、茎粗、地上和地下部分鲜质量均达到了最大值,分别为17.21 cm、5.4 mm、6.82 g和2.26 g,分别是对照的2.1倍、1.5倍、2.7倍和1.1倍;光合速率为13.95μmol/(m2·s),比A处理稍低,但无显著差异,且显著高于其他处理。综合各项指标,C处理成本低,原料来源方便,建议在黄瓜育苗中应用。 相似文献
20.
研究了闽楠扦插育苗中不同穗条母树年龄、不同扦插基质、生根剂、不同扦插时间对生根率的影响,结果表明:采条母树龄、扦插基质和扦插时间对扦插生根率影响较大;1a生穗条扦插生根率远大于3a生及5a生穗条苗;不同扦插基质对插穗生根的作用效果差异显著;砻糠灰通透性强,生根最快,成活率最高,且在后期生长上均有较佳表现;2~3月份的扦插生根率为最高。 相似文献