Spray Drying of Chitosan Acid Salts: Process Development,Scaling Up and Physicochemical Material Characterization

We investigated a spray drying process for preparing water-soluble salts of high molecular weight chitosan (CH) intended for pharmaceutical excipient applications. CH was derived from chitin of marine lobster origin (Panulirus argus). The effects of organic acid (acetic or lactic acid) and the ratio (difference) of inlet/outlet air temperature (140/90 °C or 160/100 °C) on spray drying were studied. The yield of spray-dried CH salt powders ranged from 50% to 99% in laboratory and industrial-scale processes. The spray-dried dry powder of CH salts consisted of spherical agglomerated particles with an average diameter of 36.2 ± 7.0 µm (CH acetate) and 108.6 ± 11.5 µm (CH lactate). After dispersing the spray-dried CH salt powder samples in purified water, the mean particle sizes obtained for the CH acetate salts were 31.4 nm (batch A001), 33.0 nm (A002) and 44.2 nm (A003), and for the CH lactate salts 100.8 nm (batch L001), 103.2 nm (L002) and 121.8 nm (L003). The optimum process conditions for spray drying were found: an inlet air temperature of 160 ± 5 °C, an outlet temperature of 100 ± 5 °C and an atomizer disk rotational speed of 18,200 min⁻¹. The X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) results confirmed the amorphous state of the CH salts. The ¹H nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectra of CH acetate and lactate salts verified that the spray drying process does not affect the polymer backbone. In conclusion, both laboratory and industrial-scale spray drying methods for preparing water-soluble acid salts of CH are reproducible, and the physicochemical properties of the corresponding CH acid salts are uniform.     

烤烟苯丙氨酸类致香物质与土壤理化性状的典型相关分析

通过分析河南省12个烟区土壤与烟叶各335个样品的测试数据,采用典型相关分析方法研究了烤烟苯丙氨酸类致香物质含量与土壤理化性状之间的关系.结果表明:苯甲醛、苯乙醇与土壤速效钾含量、粒径0.05 ～ 0.001 mm的土壤颗粒含量呈正相关,与pH、镁、钙、铁、铝、钠含量呈负相关;苯甲醇与速效钾含量呈负相关,与钠含量呈正相关;苯乙醛与土壤理化性状的各项指标无显著相关性.     

Effects of organic fertilizers on yam productivity and some soil properties of a nutrient-depleted tropical Alfisol

Field experiments were conducted at Owo, southwest Nigeria to select organic fertilizer treatments most suitable for sustaining high soil fertility and yam productivity on a nutrient-depleted tropical Alfisol. Eight organic fertilizer treatments were applied at 20 t ha^?1 with a reference treatment inorganic fertilizer (NPK 15–15–15) at 400 kg ha^?1 and natural soil fertility (control), laid out in a randomized complete block design with three replications. Results showed that organic fertilizers significantly increased (p = 0.05) tuber weight and growth of yam, soil and leaf N, P, K, Ca and Mg, soil pH and organic C concentrations compared with the NSF (control). The oil palm bunch ash + poultry manure treatment increased tuber weight, vine length, number of leaves and leaf area of yam by 66, 25, 21 and 52%, respectively, compared with inorganic fertilizer (NPK) and 37, 22, 19 and 44%, respectively, compared with poultry manure alone. Sole or mixed forms of organic fertilizers showed significant improvement in soil physical conditions compared with IF (NPK) and NSF (control). Synergistic use of oil palm bunch ash + poultry manure at 10 t ha^?1 each was most effective for sustainable management of soils and for improving agronomic productivity of yam.     

Soil properties and yam yield under different tillage systems in a tropical Alfisol

In Nigeria, information is lacking regarding the most suitable tillage method in extensive yam production. Hence, five tillage methods were compared at two sites in 2008–2010 with reference to their effects on soil physical and chemical properties, leaf nutrient concentrations, growth and tuber yield of yam (Dioscorea rotundata Poir) on Alfisols at Owo (site A) and Akure (site B), south-west Nigeria. The tillage methods were: zero tillage (ZT), manual ridging (MR), manual mounding (MM), ploughing + harrowing (P + H) and ploughing + harrowing + ridging (P + H + R). P + H + R had lower soil bulk density than other tillage methods and resulted in higher leaf N, P, K, Ca and Mg and yam tuber yield. In ZT, bulk density, soil moisture content, soil organic C, N, P, K, Ca and Mg were significantly higher and temperature lower than other tillage methods. Results of multiple regressions revealed that bulk density significantly influenced the yield of yam rather than soil chemical properties. Compared with MR, MM, P + H and ZT, and averaged across years, P + H + R increased yam tuber yield by 12.3, 12.8, 34.9 and 50.7%, respectively, in site A and 12.9, 13.5, 25.2 and 44.5%, respectively, in site B. P + H + R was found to be most advantageous and is therefore recommended for yam cultivation.     

Seasonal dynamics of soil microbial biomass C shows close correlation with environmental factors in natural Fagus crenata forests

Abstract

Soil microbial biomass (C_mic) is an important factor regulating a number of ecosystem processes. In this study, we investigated seasonal variations in soil microbial biomass in natural climax beech (Fagus crenata) forests in a typical cold-temperate mountain region of Japan. Four permanent tower sites along an altitudinal gradient were selected and soil samples were collected once every month during the growing season of 2007. Soil microbial biomass (by fumigation-extraction method) and soil properties were later measured in the laboratory, while environmental factors (soil temperature, soil moisture) were continuously recorded in the field. Our results indicated large seasonal variations (130.4 ~ 5558.0 µg g^?1) in soil microbial biomass in beech forests – a range that is much larger than previously reported. Statistically significant correlations are noted between soil properties with C_mic, but largely due to spatial linkages. On the other hand, the environmental factors of soil temperature and especially soil moisture largely control seasonal variations in C_mic. Furthermore, pH could be an important factor influencing seasonal change in C_mic at the 20–30 cm deep soil layer. The study suggests no direct correlation between plant eco-physiology and soil microbial biomass in seasonal courses of the forests.     

Radiation-use efficiency in leys: influences of growth period and clover proportion

Abstract

Acquisition of carbohydrates and their partitioning to storage organs are decisive attributes for the persistence of perennial leys. It was therefore of interest to analyse radiation-use efficiency (RUE) and root allocation (b_r) in one-, two-, and three-year-old red clover–grass leys as functions of growth period (first and second growth, ley age), proportion of clover, and site properties (location, soil type, humus content). By calibrating RUE and b_r, the ley yields simulated with a growth model were adjusted to the values observed during two consecutive years at 33 organic farms located in the southern and the northwestern coastal regions of Finland. Driving variables of the simulations were daily measurements of weather. RUE declined from the first to the second growth and further with ley age, while its variability increased. The value of b_r decreased from the first to the second growth, and was positively correlated with RUE. The high variability of RUE between different sites, and the absence of correlations between site properties and RUE, were mainly attributable to differences in overwintering and regrowth conditions. Early measurements of above-ground biomass subsequent to overwintering and cutting, and the inclusion of site-specific measurements of soil properties would have increased the general validity of the calibrated parameter values. RUE correlated positively with proportion of clover. However, the increase of the proportion of clover from the first to the second growth was not strong enough to balance the concurrent decline of RUE. With increasing ley age there were two concurrent and reinforcing patterns, namely a decrease in proportion of clover and in RUE. Thus, both symbiotic N fixation and vigour of perennial red clover–grass leys declined with ley age. With regards to RUE, however, three-year-old leys were still productive.     

SEVERITY OF SALINITY ACCURATELY DETECTED AND CLASSIFIED ON A PADDOCK SCALE WITH HIGH RESOLUTION MULTISPECTRAL SATELLITE IMAGERY

We hypothesised that digital mapping of various forms of salt‐affected soils using high resolution satellite imagery, supported by field studies, would be an efficient method to classify and map salinity, sodicity or both at paddock level, particularly in areas where salt‐affected patches are small and the effort to map these by field‐based soil survey methods alone would be inordinately time consuming. To test this hypothesis, QuickBird satellite data (pan‐sharpened four band multispectral imagery) was used to map various forms of surface‐expressed salinity in an agricultural area of South Australia. Ground‐truthing was performed by collecting 160 soil samples over the study area of 159 km². Unsupervised classification of the imagery covering the study area allowed differentiation of severity levels of salt‐affected soils, but these levels did not match those based on measured electrical conductivity (EC) and sodium adsorption ratio (SAR) of the soil samples, primarily because the expression of salinity was strongly influenced by paddock‐level variations in crop type, growth and prior land management. Segmentation of the whole image into 450 paddocks and unsupervised classification using a paddock‐by‐paddock approach resulted in a more accurate discrimination of salinity and sodicity levels that was correlated with EC and SAR. Image‐based classes discriminating severity levels of salt‐affected soils were significantly related with EC but not with SAR. Of the spectral bands, bands 2 (green, 520–600 nm) and 4 (near‐infrared, 760–900 nm) explained the majority of the variation (99 per cent) in the spectral values. Thus, paddock‐by‐paddock classification of QuickBird imagery has the potential to accurately delineate salinity at farm level, which will allow more informed decisions about sustainable agricultural management of soils. Copyright © 2011 John Wiley & Sons, Ltd.     

红麻轻质阻燃人造板的研制

本文研究了红麻轻质阻燃板的生产技术,产品理想的生产工艺参数为:阻燃剂用量14%、成形压力4.0MPa、胶粘剂用量10%、热压时间为60s.mm-1热压温度120℃。产品的力学性能和阻燃性能指标如下:静曲强度≥31.85MPa;密度≤0.52g.cm-3;氧指数41%～50%;2分钟热释放率≤80kW.min.m-2,达到了相关标准的要求。     

Effect of hydrostatic pressure and temperature on the chemical and functional properties of wheat gluten: Studies on gluten, gliadin and glutenin

The effect of hydrostatic pressure (0.1–800 MPa) in combination with various temperatures (30–80 °C) on the chemical and physical properties of wheat gluten, gliadin and glutenin was studied. Chemical changes of proteins were determined by extraction, reversed-phase high-performance liquid chromatography (HPLC), sodium dodecylsulphate (SDS) polyacrylamide gel electrophoresis (PAGE), circular dichroism (CD) spectroscopy, thiol measurement and studies on disulphide bonds. Rheological changes were measured by extension tests and dynamic stress rheometry. Treatment of gluten with low pressure (200 MPa) and temperature (30 °C) increased the proportion of the ethanol-soluble fraction (ESF) and decreased gluten strength. The enhancement of both pressure and temperature provoked a strong reduction of the ESF and the thiol content of gluten. Within gliadin types, cysteine containing α- and γ-gliadins, but not cysteine-free ω-gliadins were sensitive to pressure and were transferred to the ethanol-insoluble fraction. Disulphide peptides isolated from treated gluten confirmed that cleavage and rearrangement of disulphide bonds were involved in pressure-induced reactions. Increased pressure and temperature induced a significant strengthening of gluten, and under extreme conditions (e.g. 800 MPa, 60 °C), gluten cohesivity was lost. Isolated gliadin and glutenin reacted differently: solubility, HPLC and SDS-PAGE patterns of gliadin having a very low thiol content were not influenced by pressure and heat treatment; only conformational changes were detected by CD spectroscopy. In contrast, the properties of isolated glutenin having a relatively high thiol content were strongly affected by high pressure and temperature, similar to the effects on total gluten.     

Semi-sweet biscuit making potential of soft wheat flour patent,middle-cut and clear mill streams made with native and reconstituted flours

The influence of patent, middle-cut and clear flour grades as native or reconstituted flour blends on both the rheological properties of the dough and the quality of semi-sweet biscuit (flour/sugar/fat/water ratio of 100/30/8/36) was studied. Moving from the central portion (patent) to the peripheral portion (clear) of the grain endosperm increased the dough hardness from 3.77 to 4.84 N, consistency from 19.3 to 25.5 N s, elongational viscosity from 4.13×10⁻⁵ to 5.54×10⁻⁵ Pa s, half-relaxation time from 0.45 to 0.59 s, but decreased the rate of relaxation from 4.51 to 3.09 s⁻¹ of the biscuit's dough produced with the native flours due to the wide variation in the physico-chemical properties of these fractions. Quantitatively, the fractionation/reconstitution procedure reduced moderately these rheological parameters, and the flour functionality could not be restored completely. Biscuits produced with the patent flour showed the largest length and lowest thickness, whilst the clear fraction led to production of denser biscuits with greater cohesion (mean tearing force) of the biscuit inner structure and also contain more grains or group of grains per unit of penetration (number of spatial ruptures). The biscuits made with the reconstituted flour fractions had almost equivalent dimensional characteristics, and excellent surface appearance, but were also darker in colour than their native flour counterparts.