水肥一体化技术在苹果园中的应用研究

针对目前山东省内多数苹果园存在的规模化、机械化管理水平不高,传统灌溉施肥模式废水废肥等问题,提出结合实际情况在不同规模的苹果园内应用不同模式的水肥一体化系统,介绍了不同水肥一体化模式的适用范围,总结了苹果园应用水肥一体化技术具有节约水肥药、改善苹果品质、保护土壤等优点,对促进山东省苹果生产方式转型升级、实现苹果产业绿色高效高质发展具有积极作用。     

江河源区高寒草甸退化草地恢复与重建技术研究

通过对江河源区高寒草甸退化草地现状的调查研究,将这一地区的退化草地分为轻度、中度、重度和极度退化4类,对不同退化演替阶段的草地采取不同治理措施进行试验研究。最后提出了通过禁牧封育、毒杂草防除和草地施肥的措施进行轻度和中度退化草地的恢复改良。通过建植人工草地和半人工草地的手段恢复与重建重度和“黑土型”退化草地的技术方案。     

Ammonia dynamics in relation to hatching in Atlantic halibut (Hippoglossus hippoglossus L.)

Total ammonia content (T_Amm) and excretion (J_Amm), and ionic content (K⁺, Na⁺, Cl_-) have been studied in embryos and yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) maintained in darkness (6 °C and 34% S) or in light (light-arrested, nonon-hatching embryos). The T_Amm of the eggs increased from 250-300 nmol ind^-1 during the first 5-10 days post fertilization (dPF) to 400-500 nmol ind^-1 at the time of natural hatching in darkness. T_Amm did not start to decrease before 2-5 days after hatch, and yolk-sac larvae had a higher T_Amm compared to the light-arrested embryos of the same age and batch. J_Amm increased exponentially rom about 0.05 nmol ind^-1 h^-1 at 10 dPF to about 3 nmol ind^-1 h^-1 at hatch. Embryos undergoing hatching showed a significantly higher J_Amm than non-hatching, light-arrested embryos of the same age. The content of K+ in distilled water rinsed eggs declined from about 0.85 µmol ind^-1 at 2-4 dPF to about 0.15 µmol ind^-1 at hatch, while that of Cl^- remained constant at about t 0.85 µmol in^-1, and that of Na⁺ increased slowly from 0.10 to 0.20 µmol ind^-1 . Based on the measured ions, there was an anionic deficit in the egg, especially during the first week of development. When exposed to increased ambient total ammonia (0-27 mM NH4Cl), no mortality occurred, and no significant increase was found in the T_Amm of the Atlantic halibut egg before 9.2 dPF despite an estimated large inward diffusion gradient for the un-ionized ammonia species (NH₃). The delayed release of T_Amm comp ared to embryos and yolk-sac larvae of other marine teleosts, may relate to the deep-water spawning of the Atlantic halibut, and the buoyancy regulation of the yolk-sac larvae.     

半干旱区旱地不同覆盖方式对糜子耗水和产量的影响

为探讨半干旱区旱地不同覆盖方式对糜子耗水特征和产量的影响,测定了全膜覆土穴播(PMS)、全膜覆盖穴播(PM)和露地穴播(CK)3种不同处理的糜子发育动态、0—200cm土壤水分季节变化、糜子地上生物量动态、产量和耗水量。2a的试验结果表明,地膜覆盖后糜子营养生长期缩短,生殖生长时期延长,但全生育期缩短;PMS处理的糜子的生育期较CK缩短12～13d,较PM延长10～11d。地膜覆盖使糜子拔节前0—200cm土层的土壤含水量增加,随糜子生育进程的推进,3种处理的耗水量依次为:PM>PMS>CK。在抽穗前,PM的地上生物量最大,其次为PMS,CK最小;在收获期,PMS的生物量和籽粒产量均显著高于PM。PM和PMS的产量较CK分别在2009和2010年提高了83.53%和64.56%,115.51%和84.47%;与PM处理相比,PMS的糜子产量在2009年和2010年提高了17.42%,18.18%,但两个处理的土壤耗水量在2a均无显著差异。因此,通过覆盖降低棵间蒸发是提高旱地糜子产量和水分利用效率(WUE)的主要途径。通过覆盖方式的选择来调控糜子的发育进程和耗水过程,对提高糜子产量和WUE也有重要意义。     

基于RS与GIS的湿地保护有效性分析——以向海自然保护区为例

向海自然保护区是以保护丹顶鹤等珍稀水禽和蒙古黄榆为主的国家级湿地自然保护区,具有重要的国际地位与作用。但是,湿地资源的退化现状使得人们对于保护区的有效性产生了疑问。本研究借助RS与GIS技术,利用1954年、1986年和2004年的土地利用数据,对比分析了向海自然保护区建立前后、保护区内各功能区、保护区及其周边区域的湿地资源动态变化,另外,结合保护区野外调查资料,探讨了湿地生境退化对鹤类等珍稀水禽迁徙及繁殖数量变化的影响。结果表明:保护区建立后湿地损失速度有所减缓,但仍保持减少的趋势;核心区内湿地得到了较好的保护,对实验区与缓冲区的保护作用较弱;与其周边区域相比,保护区内湿地资源的时空动态变化显著;鹤类等珍稀水禽种群数量随着湿地面积的萎缩而迅速减少。区域气候环境变化是导致湿地退化的主要原因,人为活动干扰以及保护区自身建设与管理中存在的问题是影响保护区对湿地资源发挥有效保护作用的主导因素。     

水产动物肌酸营养的研究进展

肌酸(creatine,Cr)又称为N-甲基胍乙酸或肌肉素,是一种含氮有机酸。已广泛应用于改善人类健康,尤其用于促进肌肉生长。本文综述了肌酸在动物体内的合成、转运和储存规律,以及肌酸在肌肉和肝脏能量代谢中的作用,分析了饲料补充肌酸对动物生长和屠宰性能的影响,并从肌肉pH、系水力和肉色角度综述了肌酸对肉品质影响的研究进展,最后对肌酸在水产动物中的应用前景进行了展望,以期为肌酸在水产动物营养中的研究和在饲料中的应用提供参考。     

复合动植物蛋白部分替代鱼粉对大菱鲆幼鱼生长、体成分及生理生化指标的影响

本研究设计5组等氮等能(粗蛋白为53%,能量为25KJ/g)的实验饲料,以60%的鱼粉饲料组作为对照(D1),豆粕∶花生粕∶鱼溶浆粉∶鸡肉粉(2∶1∶3∶2)的复合蛋白替代40%(D2)、50%(D3)、60%(D4)和70%(D5)的鱼粉,养殖大菱鲆幼鱼(Scophthalmus maximus L.)初始体重(53.0±0.2) g,养殖周期84 d,每天定时(08:00,16:30)投喂2次,投喂量为体重的1.5%～2%。实验结果显示,各处理组之间幼鱼存活率、饲料系数和摄食率均无显著性差异(P0.05);与对照组相比,D4和D5组增重率显著降低(P0.05);肥满度在D2组达到最高值,显著高于D3、D4和D5组(P0.05);脏体比(VSI)、肝体比(HSI)和肠体比(ISI)均在D2组达到最低值,均显著低于D5组(P0.05);复合动植物蛋白替代鱼粉对大菱鲆幼鱼全鱼水分和粗蛋白含量均无显著影响(P0.05);各替代组全鱼粗脂肪含量显著高于对照组(P0.05);全鱼灰分含量在D5组显著低于对照组(P0.05);各组间背肌水分、粗蛋白、粗脂肪和灰分含量均无显著性差异(P0.05);复合动植物蛋白替代鱼粉对鱼体肌肉非必需氨基酸和必需氨基酸总量无显著影响(P0.05);各替代组均显著提高了血清谷草转氨酶和谷丙转氨酶活性(P0.05);总蛋白浓度在D2组显著高于D4和D5组(P0.05);血糖浓度在D2和D3组显著低于其他3组(P0.05);D3、D4和D5组甘油三酯浓度和高密度脂蛋白浓度均显著低于对照组和D2组(P0.05);各替代组胆固醇和低密度脂蛋白浓度均显著低于对照组(P0.05);各组之间碱性磷酸酶浓度无显著差异(P0.05)。研究结果表明,复合动植物蛋白可有效替代50%鱼粉而不影响大菱鲆幼鱼生长性能和部分生理生化指标。     

Changes in soil and water quality at sediment–water interface of Penaeus vannamei culture pond at varying salinities

The health of the pond environment in shrimp farming is important for sustainable and profitable aquaculture, in which sediment–water interface is the most important influencing area. With this objective, the key parameters of water and sediment at the interface was studied in shrimp ponds with varying salinities and compared with the surface water and soil to understand the variations and the underlying causes. Total ammonia nitrogen and total alkalinity were higher in water at the interface as compared to surface water, whereas pH, nitrite, oxide forms of nitrogen (NO₃), phosphorus (PO₄) and oxidizing bacteria were observed to be lower. There was no significant difference with respect to macrominerals such as sodium, potassium, calcium and magnesium between water at the interface and surface. These changes are profoundly influenced by the uppermost 1.0‐cm thick sediment layer, where the maximum transition of nutrients takes place with the water column. The outcome of the study highlights the effectiveness of water sampling for metabolites at the interface, which reflects at an early stage any potential deterioration of pond environment, which will help the farmer to initiate timely mitigation measures.     

Does sand content in spawning substrate result in early larval emergence? Evidence from a lithophilic cyprinid fish

The spawning success of lithophilic salmonids is strongly influenced by the fine sediment content (“fines”) of spawning substrates, yet knowledge on the impacts of fines on the spawning of non‐salmonid lithophiles remains limited, despite their ecological and socio‐economic importance in European rivers. Consequently, the aim here was to use an ex‐situ experiment to investigate the impact of sand content on egg survival and timing of larval emergence of the surface‐spawning cyprinid European barbel Barbus barbus. Thirty incubator boxes within a recirculating system were filled with one of five experimental sediment mixtures (0%–40% sand by mass) that each contained 300 fertilised eggs at a depth of 50 mm. Emerged, free‐swimming larvae were captured and counted daily to assess grain‐size effects on larval survival and emergence. Specifically, total proportion of emerged larvae, cumulative daily proportion of emerged larvae and time required to reach 50% emergence were measured during the study. Whilst the proportion of sand in the sediments did not have a significant impact on egg‐to‐emergence survival (mean survival per treatment 75%–79%), it significantly affected the timing of larval emergence to the water column; early emergence was detected in treatments with elevated sand content (on average, 50% emergence after 12–13 days versus 19 days in the control). Similar to findings from salmonid studies, these results suggest high sand content in spawning gravels can influence timing of larval emergence and potentially cyprinid lithophilic fish survival.