播种期液态肥精量深施系统喷肥装置研制

针对播种期液态肥精量深施的技术需求,该文提出一种液态肥对耙点施系统,并针对喷出的液态肥需满足较佳集束性和施肥量等要求,设计一种液态喷肥装置。在Fluent软件中建立锥直型、圆锥型和圆柱型三种不同形状喷嘴的流体仿真模型,对喷嘴的非淹没射流过程进行仿真,分析了不同喷嘴喷出的液态肥速度和总压的变化,得出不同喷嘴的集束性能,确定了圆锥型为最佳喷嘴形状。选用圆锥型喷嘴,以施肥量为评价指标,以液泵压力和喷嘴直径为试验因素,设计了二因素五水平全因子试验,建立施肥量与液泵压力、喷嘴直径之间的回归方程。仿真结果表明,液泵压力和喷嘴直径对喷肥装置的施肥量影响显著(P0.01),其中,喷嘴直径对施肥量的影响程度更大;台架试验结果表明,实际试验施肥量与仿真结果变化趋势一致,施肥量试验值与修正后的回归方程预测值整体平均误差为1.62%,基本满足施肥量要求。该研究为种期液态肥精量深施的进一步研究奠定了基础。     

低磷胁迫下水稻不同品种根系有机酸分泌的差异

以4个对低磷反应不同的典型水稻品种（系）为材料，对其在低磷胁迫下根系有机酸分泌这一根系生理特性进行了比较研究。结果表明，低磷胁迫能促使水稻根系分泌更多的有机酸，这在耐低磷品种中表现尤为明显。     

Effect of organic mulches on soil bacterial communities one year after application

The application of organic mulches as a soil cover is effective in improving the quality of soil. However, very little information is available on the effect of mulches on the soil microbial community. In this study, we investigated the effect of various organic mulches on soil dehydrogenase activity (DHA) and microbial community structures in the top 1 cm and 5 cm below the soil surface 1 year after application of the mulches. DHA was stimulated at both depths in plots mulched with grass clippings (GC), but was not significantly different from the control for the other mulch treatments. Fatty acid methyl ester (FAME) analysis and denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction-amplified 16S rDNA fragments were used to assess changes in the soil microbial community structure. Cluster analysis and principle component analysis of FAME profiles showed that only soil mulched with pine chips distinctively clustered from the other treatments. At the soil surface, bacterial DGGE profiles revealed that distinct shifts in several bacterial populations occurred in soils mulched with GC and eucalyptus yardwaste (EY), while DGGE profiles from soil at the 5 cm depth revealed no distinct changes. Changes in bacterial diversity at the soil surface under different mulches were calculated based on the number of bands in the DGGE profile using the Shannon-Weaver index of diversity ( H). Compared to the control ( H =0.9), the GC- and EY-treated soils showed slightly increased bacterial diversity, with an H of 1.1 and 1.0, respectively. These results indicate that the long-term effect of organic mulches on the soil microbial activity and community structure is highly dependent upon the type of mulch and is mostly exerted in the top few centimeters of the soil profile.     

高产小麦施肥效应的研究

１９８８～１９９０年，应用１５Ｎ研究了山东省高产小麦的氮肥施用技术及其增产效应。两年各处理的试验结果趋势一致，高产小麦施用氮肥的增产作用，既决定于小麦对氮肥利用率的提高，又决定于氨肥对小麦产至的生产效应。每公顷施纯氮１８７．５ｋｇ，其中１／３作底肥，２／３作沟追肥，是高产小麦较为合理的施肥技术，我们应用此技术，小麦产量两年分别达到７７４７．５ｋｇ／ｈａ和８２５１．５ｋｇ／ｈａ。     

Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus

Summary The distribution of phosphatase activity and of phosphate fractions of the soil in the proximity of roots was studied in order to evaluate the significance of phosphatases in P nutrition of various plants (Brassica oleracea, Allium cepa, Triticum aestivum, Trifolium alexandrinum). A considerable increase in both acid and alkaline phosphatase activity in all the four soil-root interfaces was observed. Maximum distances from the root surface at which activity increases were observed ranged from 2.0 mm to 3.1 mm for acid phosphatase and from 1.2 mm to 1.6 mm for alkaline phosphatase. The increase in phosphatase activity depended upon plant age, plant species and soil type. A significant correlation was noticed between the depletion of organic P and phosphatase activity in the rhizosphere soil of wheat (r = 0.99^**) and clover (r = 0.97^**). The maximum organic P depletion was 65% in clover and 86% in wheat, which was observed within a distance from the root of 0.8 mm in clover and 1.5 mm in wheat. Both the phosphatases in combination appear to be responsible for the depletion of organic P.     

Temperature responses of NO and N2O emissions from boreal organic soil

Both NO and N₂O are produced in soil microbial processes and have importance in atmospheric physics and chemistry. In recent years several studies have shown that N₂O emissions from organic soils can be high at low temperatures. However, the effects of low temperature on NO emissions from soil are unknown. We studied in laboratory conditions, using undisturbed soil cores, the emissions of NO and N₂O from organic soils at various temperatures, with an emphasis on processes and emissions during soil freezing and thawing periods. We found no soil freezing- or thawing-related emission maxima for NO, while the N₂O emissions were higher both during soil freezing and thawing periods. The results suggest that different factors are involved in the regulation of NO and N₂O emissions at low temperatures.     

CO2 emission in an intensively cultivated loam as affected by long-term application of organic manure and nitrogen fertilizer

A long-term field experiment was conducted to examine the influence of mineral fertilizer and organic manure on the equilibrium dynamics of soil organic C in an intensively cultivated fluvo-aquic soil in the Fengqiu State Key Agro-Ecological Experimental Station (Fengqiu county, Henan province, China) since September 1989. Soil CO₂ flux was measured during the maize and wheat growing seasons in 2002-2003 and 2004 to evaluate the response of soil respiration to additions and/or alterations in mineral fertilizer, organic manure and various environmental factors. The study included seven treatments: organic manure (OM), half-organic manure plus half-fertilizer N (NOM), fertilizer NPK (NPK), fertilizer NP (NP), fertilizer NK (NK), fertilizer PK (PK) and control (CK). Organic C in soil and the soil heavy fraction (organo-mineral complex) was increased from 4.47 to 8.61 mg C g⁻¹ and from 3.32 to 5.68 mg C g⁻¹, respectively, after the 13 yr application of organic manure. In contrast, organic C and the soil heavy fraction increased in NPK soil to only 5.41 and 4.38 mg C g⁻¹, respectively. In the CK treatment, these parameters actually decreased from the initial C concentrations (4.47 and 3.32 mg C g⁻¹) to 3.77 and 3.11 mg C g⁻¹, respectively. Therefore, organic manure efficiently elevated soil organic C. However, only 66% of the increased soil organic C was combined with clay minerals in the OM treatment. Cumulative soil CO₂ emissions from inter-row soil in the OM and NPK treatments were 228 and 188 g C m⁻² during the 2002 maize growing season, 132 and 123 g C m⁻² during the 2002/2003 wheat growing season, and 401 and 346 g C m⁻² yr⁻¹ in 2002-2003, respectively. However, during the 2004 maize growing season, cumulative soil CO₂ emissions were as high as 617 and 556 g C m⁻², respectively, due to the contribution of rhizosphere respiration. The addition of organic manure contributed to a 16% increase in soil CO₂ emission in 2002-2003 (compared to NPK), where only 27%, 36% and 24% of applied organic C was released as CO₂ during the 2002 and 2004 maize growing seasons and in 2002-2003, respectively. During the 2002/2003 wheat growing season, soil CO₂ flux was significantly affected by soil temperature below 20 °C, but by soil moisture (WFPS) during the 2004 maize growing season at soil temperatures above 18 °C. Optimum soil WFPS for soil CO₂ flux was approximately 70%. When WFPS was below 50%, it no longer had a significant impact on soil CO₂ flux during the 2002 maize growing season. This study indicates the application of organic manure composted with wheat straw may be a preferred strategy for increasing soil organic C and sequestering C in soil.     

Influence of nitrogen fertilizer forms and crop straw biochars on soil exchange properties and maize growth on an acidic Ultisol

Effects of repeated application of urea (UN) and calcium nitrate (CN) singly and together with crop straw biochars on soil acidity and maize growth were investigated with greenhouse pot experiments for two consecutive seasons. Canola straw biochar (CB), peanut straw biochar (PB) and wheat straw biochar (WB) were applied at 1% of dried soil weight in the first season. N fertilizers were applied at 200 mg N kg^?1. In UN treatments, an initial rise in pH was subjected to proton consumption through urea hydrolysis, afterwards nitrification of NH₄⁺ caused drastic reductions in pH as single UN had soil pH of 3.70, even lower than control (4.27) after the 2nd crop season. Post-harvest soil analyses indicated that soil pH, soil exchangeable acidity, NH₄⁺, NO₃^? and total base cations showed highly significant variation under N and biochar types (P < 0.05). Articulated growth of plants under combined application with biochars was expressed by 22.7%, 22.5%, and 35.7% higher root and 25.6%, 23.8%, and 35.9% higher shoot biomass by CB, PB and WB combined with CN over UN, respectively. Therefore, CN combined with biochars is a better choice to correct soil acidity and improve maize growth than UN combined with biochars.     

低丘红壤有机碳库的密度及变异

在中国科学院红壤生态实验站,采样分析了不同利用方式下土壤有机C 库的密度及其变异。结果表明,低丘红壤有机C 的密度0 ~ 20cm为(2.09 0.69) kg/m2,0 ~ 100 cm为(5.01 1.46) kg/m2; 全N密度0 ~ 20 cm为(0.20 0.07) kg/m2, 0 ~ 100 cm为(0.59 0.14) kg/m2。从裸地到稀疏荒草地,0 ~ 20 cm和0 ~ 100 cm土壤有机C 密度可以提高1.0 kg/m2和1.7 kg/m2;而从稀疏荒草地到人工林地或园地,0 ~ 20 cm和0 ~ 100 cm土壤有机C 密度可以提高0.7 kg/m2和0.9 kg/m2;稀疏荒草地如果开垦利用为水田,经长期培肥达到高度熟化,则0 ~ 20 cm和0 ~ 100 cm土壤有机C 密度可以提高2.3 kg/m2和4.4 kg/m2。即使不同类型的人工林地和园地之间,0 ~ 20 cm和0 ~ 100 cm土壤有机C 的密度差异也可达到1.0 kg/m2和3.5 kg/m2。不同地形部位之间0 ~ 20 cm和0 ~ 100 cm土壤有机C 密度差异达到1.3 kg/m2和2.9 kg/m2,全N密度差异达0.1 kg/m2和0.3 kg/m2;不同肥力水平之间0 ~ 20 cm和0 ~ 100 cm土壤有机C 密度差异达到1.5 ~ 2.2 kg/m2和2.8 ~ 4.1 kg/m2,全N密度差异达0.07 ~ 0.11 kg/m2和0.20 ~ 0.23 kg/m2; 强烈侵蚀可以降低0 ~ 20 cm和0 ~ 100 cm土壤有机C 密度1.4 kg/m2和2.2 kg/m2。因此,通过调整土地利用方式,可以提高土壤有机C 库密度,增     

有机肥及DMPP对蔬菜生产及硝态氮淋失的影响

研究在等氮条件下有机无机肥配施及添加硝化抑制剂DMPP(3,4-二甲基吡唑磷酸盐)对蔬菜产量、品质及土壤硝态氮淋失的影响,旨在为蔬菜安全生产和地下水环境质量保护提供理论依据。采用大型原状土柱系统,连续种植3季蔬菜(蕹菜、苋菜和萝卜),以施有机肥的氮素量占总氮施用量的质量分数为依据,设置8个施肥处理:不施肥(CK)、纯化肥(CF)、30%有机肥+70%无机肥(30%OM)、50%有机肥+50%无机肥(50%OM)、70%有机肥+30%无机肥(70%OM)、纯化肥+DMPP(CF+DMPP)、30%有机肥+70%无机肥+DMPP(30%OM+DMPP)和50%有机肥+50%无机肥+DMPP(50%OM+DMPP)。结果表明:1)随有机肥施用比例增大,蔬菜产量呈下降趋势,但施用比例不高于50%时产量下降不显著;随有机肥施用比例增大土壤硝态氮淋失量及蔬菜硝酸盐均降低,50%OM处理土壤淋失液硝态氮平均浓度及淋失量较CF处理显著降低了29.29%和25.39%,氮肥表观利用率及表观淋失率分别为22.60%和8.82%。2)硝化抑制剂DMPP对蔬菜产量和硝酸盐含量的影响与蔬菜种类和种植季候密切相关,降低土壤硝态氮淋失的效果为CF+DMPP30%OM+DMPP50%OM+DMPP,但DMPP的抑制效果会随有机肥的比例增加而降低。50%OM+DMPP处理氮肥表观淋失率和表观利用率分别为4.70%和26.26%。3)试验期间,3季蔬菜水分输入(降雨和灌溉)分别为总水分输入量的49.82%(蕹菜季)、23.03%(苋菜季)和27.15%(萝卜季);水分淋失量为总淋失量的46.75%(蕹菜季)、19.66%(苋菜季)和33.59%(萝卜季);硝态氮淋失量为总淋失量的73.77%(蕹菜季)、2.31%(苋菜季)和23.92%(萝卜季)。研究表明,50%OM+DMPP处理,是保证蔬菜产量品质,同时有效降低土壤硝态氮淋失量的最优处理;降雨和施肥措施是影响土壤硝态氮淋失的重要因素,合理配施有机肥及添加DMPP并根据蔬菜生长需肥特性进行施肥能有效应对连续降雨造成的硝态氮大量淋失。