水稻苗期根系铵钾离子吸收的交互作用研究

【目的】为探讨水稻幼苗根系NH_4~+、K~+吸收的交互作用,深化水稻养分吸收理论,【方法】采用溶液培养的方法,对低钾及高钾浓度下水稻在有铵和无铵时的K~+吸收动力学特征进行了研究,对不同钾浓度下水稻根系NH_4~+的吸收速率进行了比较。【结果】1)当K~+0.2 mmol/L时,水稻根系通过高亲和转运系统吸收K~+服从Michaelich-Menten动力学方程;NH_4~+的存在显著降低K~+的最大吸收速率(Vmax),且降幅随着NH_4~+浓度的增加而增大;NH_4~+对水稻根表载体与K~+的亲和力(Km)影响较小,在1.62 mmol/L NH_4~+浓度下,水稻品种齐粒丝苗和沪科3号的Km分别下降了12.33%和16.46%,远低于Vmax 47.30%和39.21%的降幅。2)当K~+0.5 mmol/L时,水稻根系K~+低亲和转运系统发挥作用,K~+吸收速率随浓度的增加而不断增加,呈不饱和特征;但在相同K~+浓度下,水稻根系的K~+吸收速率随NH_4~+浓度的增加而下降。3)水稻根系对NH_4~+的吸收速率随着NH_4~+浓度的增加而增加;在相同NH_4~+浓度下,水稻根系对NH_4~+的吸收速率受K~+浓度的影响很小。【结论】NH_4~+抑制水稻苗期根系K~+的高亲和转运和低亲和转运,NH_4~+对K~+高亲和吸收的影响主要是由于铵竞争细胞膜上的钾载体所致;外界K~+浓度的变化对水稻幼苗的NH_4~+吸收速率影响很小。水稻铵钾的交互作用主要表现在NH_4~+对K~+吸收的抑制作用。     

Exogenous CaCl2 reduces salt stress in sour jujube by reducing Na+ and increasing K+, Ca2+, and Mg2+ in different plant organs

In this study, we investigated the effects of 10 mM CaCl₂ on membrane permeability, malondialdehyde (MDA), reactive oxygen species (ROS), and Na⁺, K⁺, Ca²⁺, and Mg²⁺ concentrations in different organs of sour jujube grown under 150 mM NaCl stress. Our results showed 73% leaf wilting in the Na treatment. The Na treatment significantly increased leaf superoxide (O₂^.?) production rates, hydrogen peroxide (H₂O₂) concentrations, cell membrane permeability, MDA concentrations, and Na⁺ concentrations in roots, stems, and leaves. The Na treatment significantly reduced K⁺, Ca²⁺,and Mg²⁺ concentrations in roots, but increased K⁺ concentrations in stems. Compared with the Na treatment, 39% of the leaves showed wilting symptoms in the Na+Ca treatment. The Na+Ca treatment significantly decreased leaf O₂^.? production rates, H₂O₂ concentrations, cell membrane permeability, and MDA concentrations. Moreover, the Na+Ca treatment (1) significantly reduced Na⁺ concentrations in roots, stems, and leaves; (2) significantly increased root K⁺ concentrations; (3) significantly increased K⁺/Na⁺ ratios in roots; (4) significantly increased Ca²⁺ concentrations in stems and leaves, and Mg²⁺ concentrations in roots. In conclusion, exogenous CaCl₂ reduces NaCl stress in sour jujube by reducing Na+ concentrations and increasing K⁺, Ca²⁺, and Mg²⁺ concentrations in various plant organs.     

Micro-scale heterogeneity in urban forest soils affects fine root foraging by ornamental seedlings of Buddhist pine and Northeast yew

Urban soils are frequently characterized by a strong heterogeneity caused by intense anthropogenic activity and land use changes. Soil heterogeneity is commonly known to affect tree root development, but little has been detected concerning root foraging by ornamental trees in heterogeneous urban soils at micro-scale. In this study, Buddhist pine [Podocarpus macrophyllus (Thunb.) D. Don] and Northeast yew (Taxus cuspidata S. et Z.) were selected as ornamental tree species for a two-year study. In the first-year, seedlings were cultured under contrasting photoperiods to generate different morphologies. In the second year, seedlings were transplanted to pots filled with soils collected from an urban forest. Controlled-release fertilizers (N-P₂O₅-K₂O, 14-13-13) were evenly broadcasted to a half patch of the pot (heterogeneity) or to both halves (homogeneity) on the surface 5 cm beneath the pot-top at the rate of 0.135 g N seedling⁻¹. In the fertilized heterogeneous patch, larger Buddhist pine seedlings had greater dry weight, length, surface area, volume, number of tips, and morphological foraging-precision in fine roots. Compared to Northeast yew seedlings under natural photoperiod in the first year, those under the extended photoperiod had larger size, greater fine root biomass, and length but lower foraging-precision in the second year. N and P concentrations in second-year fine roots mainly increased with the availability of patches generated by fertilization for both species. In conclusion, the ability to forage for nutrients by ornamental tree seedlings in heterogeneous urban forest soils was species-specific. Buddhist pine seedlings had higher foraging precision in heterogeneous urban soils than Northeast yew seedlings due to their response to the extended photoperiod during culture.     

棉花花铃期低温对叶片PSI和PSII光抑制的影响

选用陆地棉(Gossypium hirsutum L.)品种新陆早45号,在室外盆栽至开花结铃期后,移至人工气候室,模拟新疆棉花花铃期易出现的低温逆境条件,设置处理T(16℃/10℃,昼/夜),以常温(30℃/18℃,昼/夜)处理作为对照,采用叶绿素荧光和P700同步测定技术,研究低温对棉花花铃期叶片光合机构PSII能量分配、PSI氧化还原状态及环式电子传递流的影响。结果表明,与对照相比,低温处理显著降低了棉花叶片PSII光适应状态下最大光化学量子产量(F_v'/F_m')、光化学猝灭系数(qP)和PSII有效光化学量子产量[Y(II)],并使PSII非调节性能量耗散[Y(NO)]和调节性能量耗散[Y(NPQ)]量子产量显著升高,诱导PSII发生光抑制。低温引起棉花叶片光合机构PSI受体侧限制[Y(NA)]显著下降和供体侧限制[Y(ND)]显著升高,但未引起有效的PSI复合体含量(P_m)显著降低,表明与PSII相比,棉花叶片PSI对低温不敏感。此外,低温引起环式电子传递量子产量[Y(CEF)]以及与PSII实际量子产量比率的[Y(CEF)/Y(II)]显著升高,进一步表明在低温下,光破坏防御机制中环式电子传递流对棉花PSI、PSII起着重要的保护作用,是主要的光破坏防御机制。非光化学热耗散(NPQ)和调节性非光化学热耗散[Y(NPQ)]与[Y(CEF)]具有显著的正相关关系,表明低温引起棉花花铃期叶片PSII反应中心过度关闭产生过剩的激发能,造成了PSII可逆的光抑制,环式电子传递流的响应及较高的调节性能量耗散共同保护了棉花叶片PSI和PSII免受光抑制的损伤,这可能是棉花叶片PSI对低温不敏感的重要原因。     

不同运输方式对生菜采后生理的影响

为研究运输方式对生菜采后生理及保鲜效果的影响,以‘橡生2号’生菜为研究对象,选择3种常见的运输方式:保温箱包装常温运输(A)、保温箱加制冷剂包装在常温下运输(B)、PE包装袋加纸箱包装在0℃下运输(C)进行试验。结果表明,采用C方式在0℃冷藏条件下运输的生菜,其失重率和呼吸强度均低于另外2组,VC含量和感官评价高于另外2组。因此在运输时间在12 h内可以采用A方式运输,在24 h内可以采用B方式运输,如超过24 h则最好使用C方式运输来保证生菜的货架期及品质。     

Vectorizing agrochemicals: enhancing bioavailability via carrier‐mediated transport

Systemicity of agrochemicals is an advantageous property for controlling phloem sucking insects, as well as pathogens and pests not accessible to contact products. After the penetration of the cuticle, the plasma membrane constitutes the main barrier to the entry of an agrochemical into the sap flow. The current strategy for developing systemic agrochemicals is to optimize the physicochemical properties of the molecules so that they can cross the plasma membrane by simple diffusion or ion trapping mechanisms. The main problem with current systemic compounds is that they move everywhere within the plant, and this non‐controlled mobility results in the contamination of the plant parts consumed by vertebrates and pollinators. To achieve the site‐targeted distribution of agrochemicals, a carrier‐mediated propesticide strategy is proposed in this review. After conjugating a non‐systemic agrochemical with a nutrient (α‐amino acids or sugars), the resulting conjugate may be actively transported across the plasma membrane by nutrient‐specific carriers. By applying this strategy, non‐systemic active ingredients are expected to be delivered into the target organs of young plants, thus avoiding or minimizing subsequent undesirable redistribution. The development of this innovative strategy presents many challenges, but opens up a wide range of exciting possibilities. © 2018 Society of Chemical Industry     

蓄水多坑灌施下尿素在土壤中的运移转化特性

为提高蓄水多坑灌施尿素条件下土壤氮素利用率和保护生态环境,通过室内蓄水多坑(土箱半径40 cm,高120 cm,蓄水坑半径16 cm,深度60 cm)物理模型试验,研究了蓄水多坑灌施下尿素在土壤中的运移转化特性。结果表明,土壤水分主要分布在地表以下20~80 cm,0~10 cm土层土壤含水率较小,同一土壤深度处蓄水坑壁附近土壤含水率大于0通量面处土壤含水率;同一土壤深度蓄水坑壁附近土壤尿素态氮量大于0通量面处的尿素态氮量,尿素的水解在9 d内基本完成,第7天水解最快,尿素水解与时间存在良好的对数函数关系;土壤铵态氮主要集中在40~60 cm土层土壤中,且r=20 cm处的量高于0通量面处的;而土壤硝态氮的分布趋势与铵态氮相反,随时间的延长,0通量面和r=20 cm处的土壤铵态氮质量分数均在40~60 cm和60~80 cm增幅较大,而土壤硝态氮质量分数表现出在90~100 cm湿润锋处增幅最大。     

蜜柚果实养分分配规律及与汁胞品质的关系

研究蜜柚果实中养分分配规律及与汁胞品质的关系,以期为改善蜜柚品质及柚园养分管理提供科学依据。随机取54个0.60-1.50 kg柚果,将果实分为黄皮层、白皮层、囊衣和汁胞等4个部位取样,分析各部位全氮（N）、全磷（P）、全钾（K）、可溶性氮（包括NH4+-N、NO3--N和氨基酸态氮（AA-N））和可溶性磷（SP）的浓度及汁胞中可溶性固形物（TSS）和可滴定酸（TA）的含量等指标。蜜柚果实中氮磷钾分配比例为（8.60±1.66）：（1.00±0.02）：（8.55±1.08）;其中,氮主要分配在黄皮层,磷主要分配在汁胞,钾在各部位分配相近。果实中可溶性N占TN的19.35% ± 2.67%,以AA-N为主;其中AA-N：NO3--N：NH4+-N分别为：15.45% ± 0.70%,2.55 ± 0.08%和1.35 ± 1.89%,并且NO3--N主要分配在白皮层,NH4+-N主要在汁胞,AA-N在各部位分配相近。果实中,SP含量约为全磷的10.63% ± 0.09%,主要分配在汁胞。相关分析发现,固酸比（TSS/TA）与SP极显著正相关,与NH4+-N显著负相关。果实中N、P、K及其可溶性形态在各部位分配的规律不同,建议遵循“高氮、低磷、高钾”的原则进行科学施肥。养分分配显著影响果实品质,其中SP浓度可能是影响汁胞固酸比的主要因素。     

北疆典型土壤纵向弥散系数试验

[目的]研究不同土壤质地对土壤纵向弥散系数和弥散度的影响.[方法]根据土壤物理学及水动力弥散理论,以新疆北疆较为常见的典型砂壤土、粉壤土、壤土为例,NaCI为示踪剂,采用室内一维土柱垂直入渗试验测定纵向弥散系数.[结果]不同土壤质地,渗流速度越小弥散系数越小,渗流流速越大,达到土壤溶质浓度峰值的时间越短,且峰值浓度越大...     

马铃薯响应磷胁迫机制及磷高效利用育种

无机磷是马铃薯生长和块茎发育必需的大量营养元素,广泛参与了遗传物质和生物膜的构成、能量转化以及代谢调控等过程,对马铃薯茎叶生长和块茎发育起着重要的作用.土壤的磷含量会明显影响马铃薯的发育,且在不同遗传特性的马铃薯品种间存在磷效率差异.为了保证马铃薯的正常生长和产量的稳定,磷肥在马铃薯种植业中被大量使用.然而,磷肥的过量...