六氟化硫（SF6）示踪法测定反刍动物甲烷排放的技术

详细描述了用六氟化硫示踪法测定反刍动物甲烷排放的技术，包括基本原理、仪器准备和测试条件。还研究了适用于中国的采气设备、示踪参数、测定条件等技术，本技术经济实用且易操作，现正在试用。     

Methane emission from two Indian soils planted with different rice cultivars

In a greenhouse study, methane emissions were measured from two diverse Indian rice-growing soils planted to five rice cultivars under similar water regimes, fertilizer applications and environmental conditions. Significant variations were observed in methane emitted from soils growing different cultivars. Total methane emission varied between 8.04 and 20.92gm^–2 from IARI soil (Inceptisol) and between 1.47 and 10.91gm^–2 from Raipur soil (Vertisol) planted to rice. In all the cultivars, emissions from IARI soil were higher than from Raipur soil. The first methane flux peak was noticed during the reproductive phase and the second peak coincided with the grain-ripening stage of the rice cultivars. Received: July 7, 1996     

水稻田甲烷的减排方法研究

通过多年对稻田甲烷排放量的野外实验观测和对稻田甲烷产生与排放因子的研究，总结出减少稻田烷排放的主要方法，分析了各种减排方法的效果和经济效益，并利用层次分析法对各种减排方法做了综合的评价。现有的稻田甲减排方法主要有其它肥料代替传统的有机肥，种植低甲排放的水稻品种、灌水管理等。适用中国国情的方法主要有杂交稻替代常规稻，沼渣肥替代纯有要肥，沼渣肥替代有机肥的减排效果较好，社会效益和环境效益明显优于杂交稻     

Short-term effects of nitrogen on methane oxidation in soils

 The short-term effects of N addition on CH₄ oxidation were studied in two soils. Both sites are unfertilized, one has been under long-term arable rotation, the other is a grassland that has been cut for hay for the past 125 years. The sites showed clear differences in their capacity to oxidise CH₄, the arable soil oxidised CH₄ at a rate of 0.013 μg CH₄ kg^–1 h^–1 and the grassland soil approximately an order of magnitude quicker. In both sites the addition of (NH₄)₂SO₄ caused an immediate reduction in the rate of atmospheric CH₄ oxidation approximately in inverse proportion to the amount of NH₄ ⁺ added. The addition of KNO₃ caused no change in the rate of CH₄ oxidation in the arable soil, but in the grassland soil after 9 days the rate of CH₄ oxidation had decreased from 0.22 μg CH₄ kg^–1 h^–1 to 0.13 μg CH₄ kg^–1 h^–1 in soil treated with the equivalent of 192 kg N ha^–1. A ¹⁵N isotopic dilution technique was used to investigate the role of nitrifiers in regulating CH₄ oxidation. The arable soil showed a low rate of gross N mineralisation (0.67 mg N kg^–1 day^–1), but a relatively high proportion of the mineralised N was nitrified. The grassland soil had a high rate of gross N mineralisation (18.28 mg N kg^–1 day^–1), but negligible nitrification activity. It is hypothesised that since there was virtually no nitrification in the grassland soil then CH₄ oxidation at this site must be methanotroph mediated. Received: 31 October 1997     

稻田温度与甲烷排放通量关系的研究

稻田温度与CH4 排放通量有密切关系 ,通过灰关联分析发现稻田 5cm深处温度与CH4 排放通量关系最密切 ,水稻抽穗期CH4 排放通量达极大值 ,而完熟期CH4 排放通量达极小值。温度对CH4 排放通量的增效应明显 ,减效应较弱。稻田温度与CH4排放通量的关系呈S型曲线相关关系。     

Tillage-induced environmental conditions in soil and substrate limitation determine biogenic gas production

Tillage changes soil environmental conditions and controls the distribution of residues in the soil, both actions that affect the production and emission of soil biogenic gases (CO₂, N₂O, and CH₄). The objective of this study was to determine how tillage-induced environmental conditions and substrate quality affect the mineralization rate of easily metabolizable compounds and the subsequent production of these gases. Carbon compounds, with and without nitrogen, were applied to soil cropped to maize under tilled and no-till systems. Following substrate application in the spring and summer, biogenic gases were measured periodically at the soil surface (flux) and within the profile (concentration) at 10-, 20-, and 30-cm depths (i.e., within, at the bottom of, and below the plough layer). Strong CO₂ and N₂O responses to sucrose and glycine in both the field and the laboratory indicate that the soil was C- and N-limited. Surface fluxes of CO₂ and N₂O were greater in soils amended with glycine than with sucrose and were greater in tilled than no-till soils. Transient emission of CH₄ following the addition of glycine was observed and could be attributed to inhibition of N mineralization and nitrification processes on CH₄ oxidation. Laboratory and field measurements indicated that the larger substrate-induced CO₂ emission from the tilled soils could not be attributed to differences in the total biomass or the basal respiratory activity of the soils. Thus, there appears to be no underlying difference in the functional capacity of the microbial communities under different tillage regimes. Comparison of gas profiles indicates relative accumulation of CO₂ at depth in soils under no-till, as well as greater decline in profile CO₂ content with time in the tilled compared to the no-till soil. These results support the conclusion that greater CO₂ efflux from the tilled soils resulted from more rapid gas diffusion through the profile. Hence, the observed differences in gas fluxes between tilled and no-till soils can be attributed to differences in physical environment.     

Influences of indigenous phototrophs on methane emissions from a straw-amended paddy soil

A pot experiment was conducted to investigate the influences of indigenous phototrophs on methane (CH₄) emissions from a paddy soil where rice straw was incorporated or was surface-applied. During the cultivation, half of the pots were covered with aluminum foil, except for the minimum space for rice plants, to prevent ambient light reaching the floodwater or the soil surface. Growth of oxygen-producing phototrophs was hardly observed in the unilluminated plots, whereas intensive growth of algae, duckweed and hydrophytes was found in the illuminated ones. Plant growth was not affected by the different treatments. Seasonal changes in CH₄ emission determined by a closed chamber method indicated that illumination had no or only minor effects on CH₄ emissions when rice straw was incorporated or was not applied, but significantly reduced CH₄ emissions when rice straw was surface-applied. Methanogenesis occurring in the soil-floodwater interface was further investigated in two lab-scale model experiments measuring methanogenic activity. As a result, more activated methanogenesis was found in the surface-applied rice straw and the soil around the straw compared with the soil incubated without rice straw. The magnitude of the methanogenic activity in the rice straw incubated under illuminated conditions was significantly lower than that incubated in the dark. Consequently, this study demonstrates that methanogenesis in paddy soil occurs even in the soil-floodwater interface if plant residues like rice straw exist, and such methanogenesis is likely to be suppressed by growth of indigenous phototrophs under illumination.     

稻田甲烷排放影响因素及其研究进展

CH4是大气中仅次于CO2的最重要的温室气体之一,其温室效应贡献已达15～20%。中国稻田是CH4的重要排放源,对全球大气的CH4排放起着重要的作用。本文较为详细地介绍了近几十年来国内外关于稻田CH4排放的研究进展。对稻田CH4的排放机理、CH4的排放规律及影响稻田CH4排放的各种因素作了详细分析,并相应地提出了控制稻田CH4排放的各种措施,最后提出了今后我国稻田CH4排放研究应加强的几个方面内容。     

Effect of Rice Root System on Methane Transmission Velocity

以不同水稻品种为材料 ,通过实验室手段测定水培稻株甲烷传输速率 ,研究水稻根系对甲烷传输的影响。结果表明 :在水培液中加叠氮化钠对汕优 63和冒子头的甲烷传输都有一定的抑制作用 ,但使 2个品种甲烷传输速率明显减小的浓度不一致 ,汕优 63为 0 .1× 10 - 8,冒子头为 0 .5× 10 - 8。说明品种间对叠氮化钠耐性存在差异。通过进一步对汕优 63水稻根系剪切处理表明 :剪根后甲烷传输速率随之改变 ,留根条数、根重与甲烷传输速率的变化均呈明显的线性正相关 ;仅切除根尖 ,甲烷传输速率大幅度下降 ,降低了 5 0 .8%。这些结果说明稻根的主动吸收 ,对稻体的甲烷传输起十分重要的作用     

蓝藻厌氧发酵控制因素与反应器应用研究进展

蓝藻厌氧发酵生产沼气是蓝藻资源化利用的一个重要方式。综述了蓝藻厌氧发酵产沼过程的控制因素和厌氧反应器的应用,为从工程上实现蓝藻发酵制沼气提供参考。