不同基础培养基对漏斗形马勃菌丝体生长的影响

[目的]研究漏斗形马勃在7种不同基础培养基上的生长情况。[方法]采用7种基础培养基进行试验,通过液体和固体培养,以菌丝体生物量及菌落直径为指标,确定漏斗形马勃的最佳基础培养基。[结果]最适合漏斗形马勃生长的基础培养基配方为玉米渣4%、蛋白胨0.3%、蔗糖2%、KH2PO41%、Mg SO40.05%。在28℃和摇床转速150 r/min条件下,液体培养5 d后的菌丝体干重为16.56 g/L。在28℃固体培养7 d,菌丝体鲜重和干重分别为16.47和2.27 g/L,菌落直径为4.49 cm。[结论]该研究为漏斗形马勃培养条件的进一步优化奠定了基础。     

Assessment of Uterine Vascular Perfusion During the Estrous Cycle of Mares in Connection to Circulating Leptin and Nitric Oxide Concentrations

The objective of this study was to find difference in vascular perfusion of uterine horns or uterine body throughout the estrous cycle and their relation to circulating nitric oxide and leptin concentrations. Five cyclic mares were subjected to transrectal Doppler ultrasonography and blood sampling for 18 days. Area of color and power Doppler modes was measured in pixels. Day (P = .0001) of the estrous cycle and ovulation (P = .0001) influenced uterine blood flow. Uterine body blood flow directed away from the transducer (blue, P = .0001) increased from day −5 until day 0 (day of ovulation), and its power (P = .0001) blood flow increased from day −6 until day 0; then, both decreased until days 12 and 10, respectively. Conversely to the contralateral uterine horn, ipsilateral uterine horn blood flow directed away from the transducer (blue, P = .0001) increased from day −5 until day −1, and its power (P = .0001) blood flow increased from day −6 until day 0; then, both decreased until day 10. Nitric oxide concentrations (P = .0001) attained two major peaks; the first on day −3 and the other persisted from day 2 until day 5. Leptin concentrations increased (P > .001) with a maximum value on day 0 and then decreased until a minimum value on day 9. In conclusion, during the estrous cycle, ipsilateral uterine horn and uterine body blood vessels had similar blood flow. Both leptin and nitric oxide played a role during follicle growth, ovulation, and corpus luteum development and modulated uterine blood flow before and after ovulation.     

农业社会化服务体系框架构建与机制研究

农业社会化服务体系是一个多层次、全方位的复杂系统。基于对相关文献的梳理和对我国服务体系发展状况的研究,构建了新时期下农业社会化服务体系运行框架,并对其运行机制进行了分析,提出了新时期下农业社会化服务体系的基本运行机制主要包括监督机制、竞争机制、激励机制、创新机制和利益分配机制。这对我国农业社会化服务体系的完善和顺利发展具有重要的理论意义。     

经络穴位给药系统在兽医临床上的应用

经络穴位给药系统以中医经络理论为基础,具有经穴效应和药物效应的双重治疗特性,已广泛应用于兽医临床.目前,兽医临床上常用穴位给药方式是穴位埋植和穴位注射,而常用穴位主要是后海穴、百会穴、肺龠穴.随着经络穴位给药系统的广泛应用,新型经络穴位给药制剂和经络穴位给药作用机理已成为经络穴位给药系统研究的重点.     

分娩应激对围产期奶牛免疫机能的影响

本文分析了围产期奶牛的分娩应激对其免疫机能的影响,并从宏观和微观上进行了详细的阐述,为围产期奶牛的饲养管理提供了理论依据。     

The Standardization of an Apparatus for the Mixing of Soil Samples for Nematode Extractions

Quantitative determination of nematode populations in soils frequently necessitates the mixing of representative soil samples to form a homogeneous, compound sample from which the nematodes are extracted. A mixing apparatus was developed and standardized with the aid of a spectrophotometric technique by which the dispersion of a dye through the soil during mixing could be determined. It was found that the mixer is most suitable for use on sandy loam soils. Optimal mixing is obtained at a speed of 15 rpm for 2 minutes. Under these conditions mechanical damage to nematodes during mixing is minimal.     

论房屋征收与补偿过程中的维权机制

随着现阶段城市建设速度的加快，各地因拆迁行为引发的争议越来越激烈。为了更好地协调城市发展与私人权益的关系，2011年1月21日国务院颁布了《国有土地上房屋征收与补偿条例》（简称《征收条例》，下同）。与先前的《城市房屋拆迁管理条例》相比较，《征收条例》无论在立法的价值目标、具体的实施机制方面都有很大的区别。以《征收条例》和相关立法作为制度框架，构建并逐步完善房屋征收与补偿过程中的维权机制，有助于使得包括《征收条例》在内的上述立法得以贯彻实现，从而使得利益博弈在市场经济、法治国家的轨道上进行。     

甘肃迭部国有林区生态效益补偿机制的探讨

本文从白龙江主要流域的生态现状入手，着重介绍和分析了白龙江流域迭部国有林区生态效益补偿机制的现状和问题，提出了主要的解决办法。     

参金止痢口服液对腹泻哺乳仔猪肠道微生物菌群影响的差异性分析

研究参金止痢口服液对腹泻哺乳仔猪肠道微生物菌群的影响。选取同等饲养条件下7~15日龄体重相近的“杜×长×大”三元杂交仔猪共计9头,其中有腹泻症状的6头将其随机平均分为腹泻空白组(FXK)和腹泻中药组(FZ),正常的3头为健康空白组(JK),中药组灌服参金止痢口服液,空白组灌服同等体积生理盐水,连续灌胃5d,在给药后的第8天对仔猪采血进行抗体及细胞因子水平测定,并采集结肠内容物提取样本中菌群总DNA进行16S rRNA高通量测序。结果显示：与FXK组相比,FZ组血清中IgG含量显著升高(P＜0.05),IgM含量显著降低(P＜0.05),炎性因子IL-1β、IL-6、TNF-α含量显著下调(P＜0.01)。α、β多样性分析结果表明,三个组结肠肠道菌群结构差异显著。在门分类水平上,FZ组仔猪肠道拟杆菌门相对丰度显著高于FXK组(P＜0.05),变形菌门相对丰度显著低于FXK组(P＜0.05),而FZ与JK组之间两个菌门差异不显著。属水平上,FZ组韦荣球菌属和乳杆菌属相对丰度显著低于FXK组(P＜0.05),普雷沃氏菌属相对丰度显著高于FXK组(P＜0.05),FZ组与JK组之间这三个菌属差异不显著。综上结果表明,参金止痢口服液可能通过调节免疫及炎性相关因子水平和调节肠道菌群结构从而发挥治疗哺乳仔猪腹泻的作用。     

“互联网+”背景下电子商务在饲料企业经营中的运行机制研究

电子商务的迅猛发展,不仅改变了企业的经营模式,也改变了用户的消费习惯和理念。饲料企业也开始寻求电子商务与传统交易方式相结合的经营模式,谋求企业的长远发展。本文基于"互联网+"背景下,深入分析了我国饲料企业电子商务的经营现状和存在的问题,探讨了电子商务在企业经营中的运行机制和不足之处,并提出了相关的优化对策,以期能帮助企业实现可持续发展。