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991.
Progress in mapping agronomic genes in apple (The European Apple Genome Mapping Project) 总被引:4,自引:0,他引:4
Graham J. King 《Euphytica》1994,77(1-2):65-69
Summary The progress of the European Apple Genome Mapping Project is described. Populations segregating for a range of agronomic genes
have been established in six European countries. The need for robust methods of analysis has been identified, especially with
regard to the development of molecular markers. Isozyme systems, RAPDs, RFLPs and amplified genes are being used to construct
a reference genetic linkage map. Standardisation and precise definition of both genotypic and phenotypic measurements has
been recognised as being essential for future exploitation of genetic markers in apple breeding. Phenotypic measurements are
being replicated in different geographical locations over several years. Statistical and genetic analyses are aimed at defining
components of genetic variation which account for ‘genes’, as defined by apple breeders. A relational database is being constructed
which will combine disparate sources of data relating to the genetics of apple. Comparative mapping has been identified as
an efficient means of expanding genetic knowledge within and between Rosaceae genomes. 相似文献
992.
The Triticum dicoccoides-derived wheat line Zecoi-1 provides effective protection against powdery mildew. F3 segregation analysis of Chinese Spring × Zecoi-1 hybrids showed that resistance in line Zecoi-1 is controlled by a single dominant gene. Amplified fragment length polymorphism (AFLP) analysis of bulked segregants from F3s showing the homozygous resistant and susceptible phenotypes identified eight markers, of which four were associated with the resistance allele in repulsion phase. Following the assignment of these four repulsion phase AFLP markers to wheat chromosome 2B with the aid of Chinese Spring nulli-tetrasomic lines, they were physically mapped in the terminal breakpoint interval 0.89 (2BL-6)–1.00 (telomere) of chromosome 2BL. Genetic and physical mapping of simple sequence repeat markers from the distal half of chromosome 2BL located the wild emmer-derived powdery mildew resistance gene distal of breakpoint 0.89 in deletion line 2BL-6. Based on disease response patterns, genomic origin and chromosomal location the resistance gene in Zecoi-1 is temporarily designated MlZec1. 相似文献
993.
In this study, we report the use of ISSR to assess genetic diversity and to determine the relationships among ten cultivars
of common bean developed in Argentina and three materials from France. ISSR markers resolved two major groups corresponding
to the Andean and Mesoamerican gene pools of common bean. We compared the results of previous analysis, performed with RAPD
markers (Galván et al., 2001), with the results generated by means of ISSR. It appears that ISSR are better tools than RAPDs
to identify beans by gene pool of origin though they did not revealed as many differences between individuals as RAPDs.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
994.
995.
The gene Pi15 for resistance of rice to Magnaporthe grisea was previously mapped to a ≈0.7-cM region on chromosome 9. To further define the chromosomal region of the Pi15 locus, a contig spanning the locus was constructed, in silico , through bioinformatics analysis using a reference sequence of the cultivar 'Nipponbare'. One simple sequence repeat marker adopted from the International Rice Microsatellite Initiative and six candidate resistance gene (CRG) markers, developed from gene annotation of the reference sequence of the contig, were used for linkage analysis in a mapping population consisting of 504 extremely susceptible F2 plants. The Pi15 locus was delimited to a ≈0.5-cM region flanked by the markers CRG5 and CRG2 and co-segregated with the markers BAPi15782 , CRG3 and CRG4, which was physically converted to a 44-kb interval. 相似文献
996.
Summary Genetics of resistance to Ascochyta fabae Speg. in Vicia faba L. was studied with a final objective to develop resistant faba bean varieties to Ascochyta blight. The study was conducted separately on 3 single spore isolates (AF10-2 and AF13-2 from Tunisia and AF4-3 from France) and belonging to different groups of virulence (GV1 and GV2). Important general combining ability (GCA) effects were found especially with isolates AF10-2 and AF4-3. Specific combining ability (SCA), although significant for the 3 isolates, was important only with AF13 -2, but less important than GCA. Additive gene effects were predominant to non-additive effects. Lines 29H and A8817 transmitted to their progenies resistance to the 3 isolates, whereas 14–12 and 19TB conferred resistance to their progenies only with isolates AF13-2 and AF4-3, respectively. In the material studied, resistance was generally controlled by dominant genes but also could be attributed to recessive genes although less frequent. Analysis of segregation in the F2 of 2 crosses between the resistant lines (A8817 and 29H) and the susceptible line (14–12) with isolate AF4-3 revealed dominant monogenic control at the level of leaves in the 2 resistant lines and, in addition, a recessive gene controlling resistance of stems. Non-allelic interactions were occasionally manifested and their origin appeared to be due to line 19TB. A recurrent selection scheme was proposed with the objective to develop improved open-pollination populations and synthetic varieties responding to the objective of the national Tunisian research programme on faba bean. 相似文献
997.
Summary In this study, the application of molecular markers to optimise genetic diversity in a polycross breeding program of perennial
ryegrass (Lolium perenne L.) was evaluated. The genetic diversity among 98 potential parental plants from three maturity groups (early, intermediate
and late flowering) was investigated using amplified fragment length polymorphism (AFLP) markers. For each maturity group,
two polycrosses of six parental plants with contrasting levels of genetic diversity were composed. Average genetic diversity
among parents selected for narrow polycrosses was 36% lower than among parents selected for wide polycrosses. Diversity within
first generation synthetic progenies (Syn1) was proportional to the diversity within the respective parental polycrosses.
However, differences were less pronounced with Syn1 progenies from narrow polycrosses showing 16% reduced diversity when compared
to Syn1 progenies from wide polycrosses. Multivariate analyses allowed for a clear separation of the six Syn1 progenies based
on AFLP markers and demonstrated their genetic distinctness. Evaluation of dry matter yield, date of ear emergence and stem
length of Syn1 and Syn2 progenies showed progenies from wide polycrosses to be constantly higher yielding when compared to
progenies from narrow polycrosses. On the other hand, there was no significant difference in variability for the two morphological
traits between progenies of narrow- and wide polycrosses. The results presented here provide evidence for an efficient application
of molecular markers to select genetically diverse polycross parents which resulted in an average yield increase of 3.8%. 相似文献
998.
999.
The extent of genetic diversity and the genetic relationships among 94 coconut varieties/populations (51 Talls and 43 Dwarfs)
representing the entire geographic range of cultivation/distribution of the coconut was assessed using 12 pairs of coconut
micro satellite primers. A high level of genetic diversity was observed in the collection with the mean gene diversity of
0.647±0.139, with that of the mean gene diversity of Talls 0.703±0.125 and 0.374±0.204 of Dwarfs. A phenetic tree based on
DAD genetic distances clustered all the 94 varieties/populations into two main groups, with one group composed of all the Talls
from southeast Asia, the Pacific, west coast of Panama, and all Dwarfs and the other of all Talls from south Asia, Africa,
and the Indian Ocean coast of Thailand. The allele distribution of Dwarfs highlighted a unique position of Dwarf palms from
the Philippines exhibiting as much variation as that in the Tall group. The grouping of all Dwarfs representing the entire
geographic distribution of the crop with Talls from southeast Asia and the Pacific and the allele distribution between the
Tall and Dwarf suggest that the Dwarfs originated from the Tall forms and that too from the Talls of southeast Asia and the
Pacific. Talls from Pacific Islands recorded the highest level of genetic diversity (0.6±0.26) with the highest number of
alleles (51) among all the regions.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
1000.
Levels of genetic similarity characterizing 20 grasspea (Lathyrus sativus L.) populations collected in central Italy (17 populations in the Marche region and three populations in the Abruzzo region) were analysed with amplified fragment length polymorphism (AFLP) molecular markers. Two main clusters were found: one included large‐seeded populations from farms that were not market‐oriented (named Household populations) and the second, small‐seeded populations, cultivated in market‐oriented farms (named Commercial populations). Relationships among populations collected in different regions were found, although one population of the Abruzzo region was placed between the two main clusters, suggesting a possible further genetic differentiation within this grasspea germplasm collection. Principal component analysis based on AFLP marker frequency was effective in identifying polymorphic markers showing high discriminating ability between clusters H and C. In particular, seven markers showing high positive and three markers with low negative PC1 scores showed an almost cluster‐specific distribution. These results will be useful for enhancing Italian grasspea germplasm use in plant‐breeding programmes and for extending grasspea cultivation within the sustainable agricultural systems of central Italy. 相似文献