甲状腺素对猪小肠上皮细胞miR-let-7a基因表达及细胞增殖的影响

miR-let-7a在动物细胞的分化、增殖与凋亡等方面发挥越来越重要的作用。甲状腺激素(TH)作用非常广泛,机体的每个细胞几乎都是TH作用的靶细胞,其可以促进组织分化、生长和成熟。本实验用甲状腺素(T4)浓度分别为(0、0.02、0.03、0.05、0.075、0.1、0.2μmol/L)在体外培养猪的小肠上皮细胞。结果表明:T4处理组的细胞体积形态相对于空白对照组没有明显变化;当T4添加浓度为0.03μmol/L时,细胞的增殖率显著低于其他组(P0.05);当T4浓度为0~0.03μmol/L时,let-7a的表达随着添加剂量的增加而升高,浓度从0.03~0.2μmol/L变化时,let-7a的表达呈现降低趋势,浓度为0.03μmol/L时表达量极显著高于其他组(P0.01)。let-7a的表达量与细胞增殖呈负相关。     

半胱胺对育成期水貂生长和饲料转化率的影响

选择健康育成期水貂208只,其中200只为试验组,8只为对照组,采用二因子试验设计:一个因子为半胱胺的不同添加量,分别按每千克体质量添加10、40、70、100、130 mg半胱胺;另一因子为半胱胺的不同添加间隔时间,分别为1、3、5、7、9 d 5个时间间隔.通过饲养试验研究了半胱胺对育成期水貂体质量增加、饲料转化率...     

基于茶籽壳代替木屑栽培茶树菇配方筛选试验

不同比例的茶籽壳和木屑组合成5个试验配方,比较不同配方对菌丝生长、子实体经济性状和生物转化率的影响。试验结果表明,在供试的5个配方中,配方4（棉籽壳50%、茶籽壳15%、木屑5%、麦麸20%、玉米粉7%、石膏1%、磷肥1%、石灰1%）为优选配方。     

我国蔬菜肥料利用率现状与提高对策

我国露地蔬菜种植和设施蔬菜种植养分用量均超过蔬菜生长所需,导致土壤次生盐渍化、肥料利用率低,增加了面源污染风险,在分析这些现状的基础上,对造成蔬菜种植中氮、磷、钾肥料利用率低下的原因进行了分析,如有机肥与化肥施用配比随意性强,搭配不合理;氮、磷、钾肥配施比例不合理;大量元素与中微量元素配合施用不合理;肥料总施用量过大;土壤自身的原因等。并提出了提高肥料利用率的测土与科学配方施肥、有机肥与无机肥合理配合施用、采用水肥一体化技术和土壤调理剂等措施,有望提高我国蔬菜肥料利用率,节约资源,防止面源污染,实现蔬菜生产的可持续绿色发展。     

Detection of cigarettes and other tobacco products by giant African pouched rats (Cricetomys gambianus)

If the illicit tobacco trade were eliminated, governments could gain at least $31.3 billion a year, and more than 164,000 premature deaths a year could be avoided after 2030 (Joossens, Merriman, Ross, and Raw, 2009). Dogs have been used successfully in tobacco control programs, and there is a good chance that rats could also play an important role. In the present experiment, giant African pouched rats were trained to respond to filters that had been stored together with cigarettes (i.e., soaked) and to not respond to filters that had been soaked with noncigarette items. Generalization to untrained types of tobacco was then tested. The sensitivity of 4 rats trained on filters soaked with 1 of 7 types of cigarettes ranged from 86% to 100% (mean, 95%). There was very little evidence of generalization when the rats were tested on tobacco leaves and snuff but good evidence of generalization when the rats were tested on cigarettes that had been soaked with strong-smelling additives. These findings suggest that rats may be a valuable asset in the global effort to control illicit cigarette trade.     

Relevance of Osmotic and Frost Protecting Compounds for the Winter Hardiness of Autumn Sown Sugar Beet

The cultivation of autumn sown sugar beet (winter beet) is supposed to result in a marked yield increase compared with spring sown sugar beet. Although the importance of the growth stage reached before winter for the survival of autumn sown sugar beet has already been shown, it is not clear to which extent osmotic and potentially frost protecting compounds may contribute to winter hardiness. The study thus aimed to analyse the acclimatization process of sugar beet to low temperatures and to identify compounds which are important for survival of frost. Field trials with autumn sown sugar beet were conducted at eleven environments in Germany from 2009/10 to 2012/13, which were accompanied by greenhouse experiments with controlled temperature regimes. In the field trials, the survival rates after winter varied from 0 % to 99 %, but only in four environments differences between the five genotypes occurred. During acclimatization, betaine, glutamine, proline and raffinose were markedly accumulated and osmolality was enhanced. In particular betaine, amino acids and osmolality showed a positive correlation to the survival rate and were thus identified as potentially frost protecting substances for sugar beet. In contrast, raffinose and proline seem to act rather as stress indicators as they were negatively related to survival. Possible frost protecting substances were identified which can be used in breeding to improve the winter hardiness of sugar beet.     

Pharmacokinetics of xylazine after 2-, 4-, and 6-hr durations of continuous rate infusions in horses

Intravenous (i.v.) bolus administration of xylazine (XYL) (0.5 mg/kg) immediately followed by a continuous rate infusion (CRI) of 1 mg kg⁻¹ hr⁻¹ for 2, 4, and 6 hr produced immediate sedation, which lasted throughout the duration of the CRI. Heart rate decreased and blood pressure increased significantly (p > .05) in all horses during the first 15 min of infusion, both returned to and then remained at baseline during the duration of the infusion. Compartmental models were used to investigate the pharmacokinetics of XYL administration. Plasma concentration–time curves following bolus and CRI were best described by a one-compartment model. No differences were found between pharmacokinetic estimates of the CRIs for the fractional elimination rate constant (K_e), half-life (t_1/2e), volume of distribution (V_d), and clearance (Cl). Median and range were 0.42 (0.15–0.97)/hr, 1.68 (0.87–4.52) hr, 5.85 (2.10–19.34) L/kg, and 28.7 (19.6–39.5) ml min⁻¹ kg⁻¹, respectively. Significant differences were seen for area under the curve ( ) (p < .0002) and maximum concentration (C_max) (p < .04). This indicates that with increasing duration of infusion, XYL may not accumulate in a clinically relevant way and hence no adjustments are required in a longer XYL CRI to maintain a constant level of sedation and a rapid recovery.     

果蝇引诱物的引诱效果与杨梅甜度、反光率的关系

经室内筛选,得到一果蝇引诱物,并对其进行田间引诱效果试验。结果发现,在杨梅逐渐成熟至干枯过程中,杨梅甜度先增加后减少,反光率越来越低,果蝇引诱物的引诱效果则呈现先升高后降低再升高的趋势。     

Greater humification of belowground than aboveground biomass carbon into particulate soil organic matter in no-till corn and soybean crops

Quantifying the amount of carbon (C) incorporated from decomposing residues into soil organic carbon (C_S) requires knowing the rate of C stabilization (humification rate) into different soil organic matter pools. However, the differential humification rates of C derived from belowground and aboveground biomass into C_S pools has been poorly quantified. We estimated the contribution of aboveground and belowground biomass to the formation of C_S in four agricultural treatments by measuring changes in δ¹³C natural abundance in particulate organic matter (C_POM) associated with manipulations of C₃ and C₄ biomass. The treatments were (1) continuous corn cropping (C₄ plant), (2) continuous soybean cropping (C₃), and two stubble exchange treatments (3 and 4) where the aboveground biomass left after the grain harvest was exchanged between corn and soybean plots, allowing the separation of aboveground and belowground C inputs to C_S based on the different δ¹³C signatures. After two growing seasons, C_POM was primarily derived from belowground C inputs, even though they represented only ∼10% of the total plant C inputs as residues. Belowground biomass contributed from 60% to almost 80% of the total new C present in the C_POM in the top 10 cm of soil. The humification rate of belowground C inputs into C_POM was 24% and 10%, while that of aboveground C inputs was only 0.5% and 1.0% for soybean and corn, respectively. Our results indicate that roots can play a disproportionately important role in the C_POM budget in soils. Keywords Particulate organic matter; root carbon inputs; carbon isotopes; humification rate; corn; soybean.