花生不同施钙量、密度对产量影响的裂区试验

探讨不同施钙量、不同播种密度对花生产量的影响,为生产上提供施用钙肥与合理播种密度的理论依据。采用两因素裂区试验,A(施钙量)因素为主处理,设有4个水平,即:A1为0石灰/hm2、A2为300kg石灰/hm2、A3为600kg石灰/hm2、A4为900kg石灰/hm2;B(播种密度)因素为副处理,设有4个水平,即:B124万粒/hm2、B228.5万粒/hm2、B331.5万粒/hm2、B436万粒/hm2;3次重复。实验结果表明:施用300石灰/hm2、播种密度28.5万粒/hm2,可获得较高产量,达到3202.83 kg/hm2和最高的净收入,达到11531.16元/hm2。     

惠州市生产基地农产品质量安全状况分析

2011~2013年惠州市农产品质检中心对广东省惠州市生产基地果蔬及其畜禽产品质量安全状况进行监测,结果表明：3年来生产基地的蔬果样品未出现甲胺磷等国家禁止生产、销售和使用的农药;养猪场猪尿样品β—兴奋剂监测合格率在99.67％以上、猪肝样品β—兴奋剂合格率达到100％,养鸡场中鸡肉、鸡蛋样品的磺胺及喹诺酮药物残留合格率均达100％,奶牛场中牛奶样品的三聚氰胺合格率达100％。     

1961-2009年辽宁省气温、降水变化特征及突变分析

利用辽宁省28个均一化气象站点1961-2009年逐日平均气温、最高气温、最低气温及降水量资料,采用趋势分析和Mann-Kendall检验方法,分析了辽宁省近49年的气温和降水变化特征及突变特征。结果表明:辽宁省年平均气温、年平均最高气温和年平均最低气温均呈升高趋势,升温速率分别为0.32℃/10a、0.26℃/10a和0.46℃/10a,年平均最低气温的升温最为显著,增温速率是年平均最高气温增温速率的1倍多。四个季节的升温速率冬季最大,春季次之,秋季略低于春季,夏季最不明显。年平均气温1989年发生了突变,年平均最高气温1992年发生了突变,年平均最低气温1985年发生了突变。年降水量整体上有减少的趋势,但变化并不显著。四季降水量呈冬春季增加、夏秋季减少的趋势。年降水量发生了三次突变,1967年由偏多转为偏少,1983年降水量开始大幅度增加,1996再次由多变少。     

牛疙瘩皮肤病病毒ORF132基因的克隆及其原核表达

为原核表达牛疙瘩皮肤病病毒(LDSV) ORF132并对该基因进行生物信息学分析,本研究采用PCR扩增LSDV的ORF132基因,将其亚克隆至pGEX-6p-1载体中,构建重组质粒pGEX-LSDV-ORF132.生物信息学分析表明,LSDV的ORF132与Neethling vaccine LW1959株LW132、NI-2490株LSDV132和NW-LW株LD132的相应推导氨基酸同源性分别为100％、100％和94.4％,与山羊痘病毒、绵羊痘病毒相应推导氨基酸的同源性为20.7 ％～36％.SDS-PAGE和western blot分析表明,pGEX-LSDV-ORF 132在大肠杆菌BL21中经IPTG诱导主要以包涵体形式存在,其重组蛋白大小约为48.95ku,并且与特异性抗体具有抗原反应活性.     

芜菁花叶病毒不同分离物3'-cDNA片段的克隆及序列分析

 从河北、北京、山东泰安和潍坊等地区的萝卜、大白菜、甘蓝、油菜上得到8个芜菁花叶病毒(Turnip mosaic virus,TuMV)分离物,测定了它们3'-末端cDNA片段的序列。根据衣壳蛋白基因(cp)核苷酸序列可以将这8个分离物与另外2个TuMV分离物分为3组:泰安旧镇大白菜(JZBC)、甘蓝(JZGL)和萝卜(JZLB)分离物为一组;北京(BJILB)、潍坊萝卜分离物(WFLB)与引起萝卜红心病的TuMV分离物(WFLB99)为一组;泰安范镇、河北、潍坊(WFLB04)萝卜和泰安旧镇油菜(JZYC)上的TuMV分离物为一组。农壳蛋白氨基酸序列比较结果与此基本一致,所不同的是分离物WFLB99与所有分离物的差异均较大,形成单独一个分支。有必要对TuMV的变异情况和致病机理进行深入研究。     

Flower yield performance and stability of various Rosa damascena Mill. landraces under different ecological conditions

The flower yield stability of Damask rose as an important medicinal and aromatic plant at different environments has not been well documented. In order to evaluate flower yield and stability, 35 landraces of Damask rose were studied at 8 locations in Iran during 2007–2008. Analysis of variance revealed significant differences (p ≤ 0.01) among landraces (G), environments (E), locations (L) and for landrace × environment (GE) and landrace × location (GL) interactions. Both GE and GL interactions were mainly crossover, a large portion of which was accounted for by non-linear (unpredictable) component. The landraces of IS9, YZ2, WA1, IS7 and IS1 with 3120.63, 2941.63, 2894.62, 2769.15 and 2716.92 kg/ha respectively produced the highest flower yield among studied landraces. Kerman with average flower yield of 3635.46 kg/ha produced the highest yield among studied locations. According to the results, most of landraces that originated from temperate, warm temperate and arid regions produced higher flower yield than those from cool, cool temperate, semi-arid and humid regions. The landraces of YZ2, IS5, IS8, IS4, KZ1, AR1_, IS3 and BA1 were stable and YZ2, IS5, IS8, IS4, KZ1, AR1 IS6, IS3, BA1, IS10 and YZ1 were adaptable landraces for flower yield according to Eberhart and Russell (1966) model. The presence of some high flower yield and stable landraces such as YZ2 and IS5 suggests that a genotype can demonstrate high flower yield and stability for yield simultaneously. Thus, simultaneous selection for flower yield and stability using nonparametric methods could be possible. In addition, taking into consideration flower yield and stability potential, the landraces of YZ2, IS5, IS8, IS4 and KZ1 as general stable, adaptable, and high flower yield are recommended. Furthermore, the landraces of IS9 and WA1 as high flower yield and specific adaptable landraces can be recommended for temperate and arid areas and the landraces of IS7 and IS1 for semitemperate and cool areas.     

广东省香菇栽培菌株RAPD多态性分析

对广东省5个香菇(Lentinula edodes)栽培菌株或其衍生菌株进行RAPD分析。结果表明,不同菌株间存在着丰富的遗传多样性。RAPD扩增指纹能有效分辨不同菌株的基因型,可为香菇的商业菌株质量监测提供快速有效的技术手段,为规范食用菌菌种生产管理提供科学依据。