全文获取类型
收费全文 | 912篇 |
免费 | 44篇 |
国内免费 | 59篇 |
专业分类
林业 | 70篇 |
农学 | 37篇 |
基础科学 | 129篇 |
123篇 | |
综合类 | 344篇 |
农作物 | 40篇 |
水产渔业 | 66篇 |
畜牧兽医 | 169篇 |
园艺 | 29篇 |
植物保护 | 8篇 |
出版年
2024年 | 7篇 |
2023年 | 6篇 |
2022年 | 8篇 |
2021年 | 17篇 |
2020年 | 18篇 |
2019年 | 20篇 |
2018年 | 12篇 |
2017年 | 21篇 |
2016年 | 27篇 |
2015年 | 26篇 |
2014年 | 66篇 |
2013年 | 51篇 |
2012年 | 76篇 |
2011年 | 81篇 |
2010年 | 71篇 |
2009年 | 58篇 |
2008年 | 70篇 |
2007年 | 51篇 |
2006年 | 40篇 |
2005年 | 34篇 |
2004年 | 27篇 |
2003年 | 24篇 |
2002年 | 13篇 |
2001年 | 17篇 |
2000年 | 12篇 |
1999年 | 26篇 |
1998年 | 14篇 |
1997年 | 17篇 |
1996年 | 6篇 |
1995年 | 23篇 |
1994年 | 15篇 |
1993年 | 15篇 |
1992年 | 10篇 |
1991年 | 18篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有1015条查询结果,搜索用时 15 毫秒
61.
Caroline Larsson Anne Vitger Rasmus B Jensen Peter Junghans Anne-Helene Tauson 《Acta veterinaria Scandinavica》2014,56(1)
Background
Overweight and obesity are the most common nutritional disorders in dogs and may lead to various secondary diseases and decreased lifespan. In obesity research, measurement of energy expenditure (EE) and determination of the energy requirements are essential. The objective with this study was to validate and evaluate the suitability of the oral 13C-bicarbonate technique (o13CBT) for measuring EE in dog obesity studies. A further objective was to investigate the impact of body weight (BW) reduction and changes in body composition on the EE when measured under conditions corresponding to the basal metabolic rate (BMR).Results
The EE in five privately owned, overweight dogs was measured simultaneously with the o13CBT and indirect calorimetry (IC) for comparison of the results. Two measurements per dog were performed under the same standardised conditions (i.e. fasted and resting state) at the start, and after completing a 12-week BW reduction program. Additionally, measurements of body composition by Dual-energy X-ray absorptiometry (DEXA) were conducted at the beginning and at the end of the BW reduction program. There were no differences in EE results obtained by the o13CBT and IC. Overweight and the BW reduction did not affect the estimates for the respiratory quotient (RQ) or the recovery factor for the 13C-tracer (RF), both needed when using the o13CBT. The dogs lost 16% (SD ± 2.0) of their initial BW in reduced fat mass (P < 0.001), whereas fat free mass (FFM) remained unchanged. There was no effect of the BW reduction on the determined EE expressed in kJ/kg BW/d, or in kJ/kg BW0.75/d. However, EE was lower (P < 0.001) after the BW reduction program when expressed in relation to FFM (kJ/kg FFM/d).Conclusions
Results from the present study show that the o13CBT can be a used in obesity research to determine EE in fasted dogs and under resting conditions. Furthermore, the results suggest that the BMR does not change with reduced BW in overweight dogs as long as the FFM remains unchanged. This indicates that the BMR to maintain one gram of fat is equal to maintaining one gram of FFM in overweight dogs. 相似文献62.
Background
The physiological levels of endocrine and metabolic parameters in Slovene autochthonous breeds of sheep are not yet well known, nor are the mechanisms of their adaptability and responses to climate and environmental factors.Therefore, the aim of this study was to evaluate fluctuations of cortisol, insulin and non-esterified fatty acids (NEFA) in growing ewes over an one-year period. Blood samples were collected monthly from 10 yearling Jezersko-Solchava, 10 Bovec and 10 Istrian ewes. Serum cortisol, insulin and NEFA were measured with commercial kits.Results
Mean monthly cortisol values fluctuated with low levels in summer and high levels in autumn. Significant peaked cortisol values of 25.69 ± 6.89, 14.67 ± 2.43 and 21.11 ± 7.25 μg/L in Jezersko-Solchava, Bovec and Istrian breed, respectively, were found in September (Bovec breed) and October (Jezersko-Solchava and Istrian breed). Mean monthly insulin values increased during the observation period. The highest levels of 14.60 ± 3.15, 16.03 ± 5.35 and 12.56 ± 2.52 μIU/mL in Jezersko-Solchava, Bovec and Istrian breed, respectively, were observed in the last sample collection in May. NEFA concentrations were found to be low except in some autumn and spring months. The peak values were observed in March for Jezersko-Solchava and Istrian breed (0.60 ± 0.05 and 0.66 ± 0.10 mmol/L), and in April for Bovec breed (0.71 ± 0.11 mmol/L).Conclusions
Monthly fluctuations of cortisol, insulin and NEFA were measured in all observed sheep breeds, but between-breed differences in monthly values of examined parameters were insignificant. Significantly increased serum cortisol levels were found in autumn for all breeds and were probably associated with the onset of puberty and low environmental temperature. A gradual increase of insulin level in the examined ewes was in parallel with their growth. Significantly higher NEFA values in spring suggest qualitatively insufficient feed supply during that period. 相似文献63.
T.A. Burns M.R. Watts P.S. Weber L.J. McCutcheon R.J. Geor J.K. Belknap 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(4):1280-1288
Background
In EMS‐associated laminitis, laminar failure may occur in response to energy failure related to insulin resistance (IR) or to the effect of hyperinsulinemia on laminar tissue. 5′‐Adenosine‐monophosphate‐activated protein kinase (AMPK) is a marker of tissue energy deprivation, which may occur in IR.Hypothesis/Objectives
To characterize tissue AMPK regulation in ponies subjected to a dietary carbohydrate (CHO) challenge.Animals
Twenty‐two mixed‐breed ponies.Methods
Immunohistochemistry and immunoblotting for total AMPK and phospho(P)‐AMPK and RT‐qPCR for AMPK‐responsive genes were performed on laminar, liver, and skeletal muscle samples collected after a 7‐day feeding protocol in which ponies stratified on body condition score (BCS; obese or lean) were fed either a low‐CHO diet (ESC + starch, approximately 7% DM; n = 5 obese, 5 lean) or a high‐CHO diet (ESC + starch, approximately 42% DM; n = 6 obese, 6 lean).Results
5′‐Adenosine‐monophosphate‐activated protein kinase was immunolocalized to laminar keratinocytes, dermal constituents, and hepatocytes. A high‐CHO diet resulted in significantly decreased laminar [P‐AMPK] in lean ponies (P = .03), but no changes in skeletal muscle (lean, P = .33; obese, P = .43) or liver (lean, P = .84; obese, P = .13) [P‐AMPK]. An inverse correlation existed between [blood glucose] and laminar [P‐AMPK] in obese ponies on a high‐CHO diet.Conclusions and Clinical Importance
Laminar tissue exhibited a normal response to a high‐CHO diet (decreased [P‐AMPK]), whereas this response was not observed in liver and skeletal muscle in both lean (skeletal muscle, P = .33; liver, P = .84) and obese (skeletal muscle, P = .43; liver, P = .13) ponies. 相似文献64.
Background:The experiment evaluated the effect of nutrition levels and sex on the growth performance,carcass characteristics and meat quality of F1 Angus × Chinese Xiangxi yellow cattle.Methods:During the background period of 184 d,23 steers and 24 heifers were fed the same ration,then put into a2×2×2 factorial arrangement under two levels of- dietary energy(TON:70/80%DM),protein(CP:11.9/14.3%DM)and sex(S:male/female) during the finishing phase of 146 d.The treatments were-(1) high energy/low protein(HELP),(2) high energy/high protein(HEHP),(3) low energy/low protein(LELP) and(4) low energy/high protein(LEHP).Each treatment used 6 steers and 6 heifers,except for HELP- 5 steers and 6 heifers.Results:Growth rate and final carcass weight were unaffected by dietary energy and protein levels or by sex.Compared with the LE diet group,the HE group had significantly lower dry matter intake(DMI,6.76 vs.7.48 kg DM/d),greater chest girth increments(46.1 vs.36.8 cm),higher carcass fat(19.9 vs.16.3%) and intramuscular fat content(29.9 vs.22.8%DM).The HE group also had improved yields of top and medium top grade commercial meat cuts(39.9 vs.36.5%).The dressing percentage was higher for the HP group than the LP group(53.4 vs.54.9%).Steers had a greater length increment(9.0 vs.8.3 cm),but lower carcass fat content(16.8 vs.19.4%) than heifers.The meat quality traits(shear force value,drip loss,cooking loss and water holding capacity) were not affected by treatments or sex,averaging 3.14 kg,2.5,31.5 and 52.9%,respectively.The nutritive profiles(both fatty and amino acid composition) were not influenced by the energy or protein levels or by sex.Conclusions:The dietary energy and protein levels and sex significantly influenced the carcass characteristics and chemical composition of meat but not thegrowth performance,meat quality traits and nutritive profiles. 相似文献
65.
66.
旅游业作为世界上最大的产业部门,其能源消耗和碳排放已经引起关注,其中旅游交通作为旅游业能源消耗和碳排放最主要的部门,许多学者对其进行了研究.科学全面地分析旅游交通能源消耗和碳排放有助于推动旅游交通的可持续发展,进而促进旅游业更健康地发展.分析了当前旅游交通能耗和碳排放的研究方法,然后从旅游交通能源消耗和碳排放的测度、影响因素、对环境的影响和响应对策等4个方面,综述了其主要的研究内容,最后对中国旅游交通能源消耗和碳排放的研究提出了建议. 相似文献
67.
68.
69.
为了研究目前低碳背景下如何更好地促进我国农村居民生活能源优化升级,在全国7省农村家庭抽样调查问卷所得数据的基础上,利用线性支出模型对农村居民生活能源消费的结构现状进行分析.研究表明,农村居民生活能源消费已经步入商品化时期但非商品能源的消费并未停止;煤炭、电力以及液化气等商品能源的消费随收入的增加而增加,而非商品能源(薪柴)沦为劣等品其消费随收入的增加而减少;电力以及煤炭的家庭人均消费支出较多;薪柴、煤炭、电力以及液化气的消费需求都随着自身价格的上升而减少,但对价格的变化都不太敏感,煤炭、电力以及液化气等商品能源间存在着互补关系,薪柴与其他种类的能源之间存在着明显的替代关系. 相似文献
70.
以松木粉、聚醚砜树脂为材料,用SHR-10A型高速混合机制备松木粉/聚醚砜树脂复合粉末;用HRPS-ⅢA型激光粉末烧结快速成型机,遴选出复合粉末可用于选择性激光烧结工艺参数及适宜的预热温度,烧结成型。测量了不同松木粉质量比对复合粉末力学性能的影响,观察并测量了输入不同的能量密度时,烧结试件的成型效果和力学性能。结果表明:随着松木粉质量比的增加,木塑复合材料的力学性能降低。对于松木粉质量比为20%的木塑复合粉末,当输入的能量密度为0.128 J/mm3时,复合粉末即可成型;当输入的能量密度为0.312 J/mm3时,木塑复合材料的拉伸强度达到4.8465 MPa,弯曲强度为8.2152 MPa,冲击强度为1.2574 MPa,此时力学性能最强;若输入的能量密度大于0.312 J/mm3时,出现过烧现象,力学性能下降。采用扫描电镜对烧结件断面的形貌进行了表征。 相似文献