高寒地区保温节能复合墙体的设计与施工

本文从设计、施工、实测数据三方面论述了红砖型复合墙体保温节能的优越性，并介绍了其扩大使用面积、降低基础荷载、节煤等优点，值得在高寒地区推广应用。     

电动助力转向系统机械部分建模与有限元分析

根据齿条式电动助力转向系统机械部分工作情况,用Pro/E建立简化模型,使用有限元软件ABAQUS分析球头、球头座、橡胶在施加位移情况下应力分布和变形状态,将分析结果和实际变形球头进行比较,确定装配结构中的危险区域,为转向系统提供改进依据和建议。     

Activity,molecular mass and hydrolysis on baker's yeast protein of extracellular proteases from the putative probiotic bacteria Microbacterium sp. strain 8L and Exiguobacterium mexicanum strain 8N

The bacteria Microbacterium sp. 8L and Exiguobacterium mexicanum 8N are known to improve the culture of Artemia franciscana using baker's yeast as food. Using spectrophotometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE), substrate‐SDS‐PAGE and pH‐stat in vitro‐digestibility assays, the activity, molecular mass and hydrolysis on baker's yeast protein of proteases from extracellular polymeric substances (EPS) of the strains 8L and 8N along with the pathogenic strains Microbacterium sp. 8R and Vibrio parahaemolyticus 588 CECT (Vp) were studied. The EPSs of 8L and 8R showed one activity band, on which the serine inhibitor phenylmethylsulphonyl fluoride (PMSF) had no effect. The EPSs of 8N showed four bands; two were unaffected by PMSF, whereas one was affected, and the other was partially affected. The EPSs of Vp showed two bands, one partially inhibited by PMSF. No inhibitory effects from 1‐chloro‐3‐tosylamido‐7‐amino‐2‐heptanone (trypsin inhibitor) were observed in the protease bands of the studied bacteria. The EPSs of 8L and 8N showed a similar degree of hydrolysis (pH‐stat). The EPSs of 8L had the lowest Dice index of similarity of yeast protein profiles at 1 h of reaction. We conclude that the strain 8L could benefit A. franciscana by providing bacterial proteases for digestion of baker's yeast.     

日光温室前沿苯板外护增地温效果研究

日光温室前沿设置苯板外护与未设置苯板外护相比,前沿基础苯板外护可以有效地阻止热量横向传导,起到保温隔热作用,哈尔滨地区建造温室基础苯板外护深度应以1 100 mm为建筑参考。     

来源于腌干鱼的乳酸菌中抗氧化酶及胞外多糖研究

为研究从腌干鱼中获取的乳酸菌在细胞内外起抗氧化作用的主要活性物质,通过测定菌株胞内的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及提取胞外多糖(EPS)并测定其抗氧化性,利用超高效液相色谱法(UPLC)分析其结构。结果显示,9株菌的SOD、GSH-Px和CAT的最高活性分别为44.67、15.26和1.23 U/mg prot,对应菌株为L21、L4和L21;9株菌的EPS产量为(5.71±0.18)~(50.33±1.89)mg/L,L21的EPS最高纯度为56.16%±1.08%。L21中EPS的DPPH自由基清除能力和还原能力较高,综合抗氧化能力相对较强,结构分析发现其含有甘露糖(Man)、鼠李糖(Rha)、葡萄糖(Glu)和半乳糖(Gal)等4种单糖。研究表明,分离自腌干鱼的乳酸菌中,抗氧化能力越强的菌株细胞内表现出越高的抗氧化酶活性,同时细胞外EPS的产量和纯度也较高,并且EPS也具有较强的抗氧化能力。从中筛选出一株综合抗氧化能力最强的L21,可以进一步作为生物源性抗氧化剂。     

3种无机盐对念珠藻生长和胞外多糖分泌的影响

为更好地开发和利用地木耳,探讨了不同浓度的NaCl、NaNO3、Ca(NO3)2对地木耳生物量积累和胞外多糖产生的影响。结果表明:NaCl和NaNO3诱导子在低浓度时起促进作用,高浓度时抑制地木耳的生长和胞外多糖的产生,均以20 mmol/L浓度为最佳的诱导条件;而Ca(NO3)2随浓度的增加生物量和多糖含量上升,80 mmol/L诱导作用效果最好。     

电动助力转向系统简介

汽车电动助力转向系统是汽车助力转向方式之一,近年来在汽车特别是在乘用车上得到越来越广泛的应用,其工作性能优劣直接影响到汽车的操纵稳定性和安全性。文章首先介绍了电动助力转向系统的发展历程,其次阐述了电动助力转向系统是如何分类的,最后对电动助力转向系统研发过程中的一些关键技术进行了说明。     

Study on the Exterior Wall External Thermal Insulating Mortar Made from Waste Expanded Polystyrene(EPS)

The surface of modified EPS can be changed from the character of water-repellent to hydrophilic,and be wetted by freshly mixed portland cement paste.A new thermal insulating mortar with good properties of workability,thermal insulation,climate-resisting and crack-resisting can be made modified EPS as light aggregate and cement as binder,and modified with fiber and polymer.This new type of insulating mortar can be used as exterior wall thermal insulation,which breaks the limitation of traditional insulating mortar only as interior insulation.It has been widely used in civil engineering.     

淫羊藿多糖的硫酸化修饰及其对IBDV感染细胞的影响

按正交试验设定氯磺酸-吡啶法的9种修饰条件,对淫羊藿多糖（EPS）进行硫酸化修饰,得到9种硫酸化淫羊藿多糖（sEPS）,用MTT法比较了9种sEPS抵抗鸡传染性法氏囊病毒（IBDV）感染鸡胚成纤维细胞（CEF）的作用。结果表明,硫酸化修饰后sEPS较未修饰的EPS显著提高了CEF抵抗IBDV感染的作用,以sEPS2和sEPS2的效果较好,且与sEPS的硫酸基取代度和糖含量有一定的相关性,反应温度80℃、氯磺酸与吡啶的配比1：8、反应2h为最佳修饰条件。