苹果低氧气调新组合贮后效应的利用研究

利用0.12mm聚乙烯薄膜大帐进行低氧气调新组合的较大规模应用试验,对2394kg玫瑰红苹果进行低氧处理,帐内O2浓度可下调至约2%,CO2浓度可自动维持在5%～8%的较理想状态,处理结束后很易转为MA自发气调。低氧处理后再MA贮藏的苹果,虎皮病果率低、症状轻,腐烂果少,果柄和果皮新鲜,叶绿素保持效果好,果肉硬度明显高于小包装气调和冷藏对照。说明利用塑膜大帐进行前期低氧新组合处理与后期自发气调、实现低氧气调新组合贮后效应的利用是可行的。     

利用ZXX-65真空循环熏蒸设备熏蒸仓储害虫的研究

用ZXX-65真空循环熏蒸设备在不同真空压力、熏蒸时间、药剂浓度条件下对选定的典型仓储实验试虫进行熏蒸，根据杀虫效果，筛选出害虫的最优熏蒸条件组合。结果表明，ZXX-65真空循环熏蒸设备可以在2h内杀死各种仓储害虫。40000Pa，60g/m^3，2h为仓储害虫的最优熏蒸条件组合。     

中国苜蓿育成品种遗传多样性及亲缘关系研究

以苜蓿的种子贮藏蛋白的多态性为研究指标,对中国14个审定登记的苜蓿育成品种的遗传多样性及亲缘关系进行了研究。研究结果表明:蛋白单体的分子量介于21.2～85.1kD之间,蛋白单体电泳图谱多态性品种间无显著的特异性;在参试的14个育成品种中,品种内基因多样性平均值(HS)为0.3136,总基因多样度(HT)为0.3741,基因分化系数(GST)为0.162;以λ=3.700为标准划分,14个育成品种划分为六个组群,各组群划分结果主要与参试品种的亲本来源有关,而与其种植地、育种地关系不大。     

Canine GM2‐Gangliosidosis Sandhoff Disease Associated with a 3‐Base Pair Deletion in the HEXB Gene

Background

GM2‐gangliosidosis is a fatal neurodegenerative lysosomal storage disease (LSD) caused by deficiency of either β‐hexosaminidase A (Hex‐A) and β‐hexosaminidase B (Hex‐B) together, or the GM2 activator protein. Clinical signs can be variable and are not pathognomonic for the specific, causal deficiency.

Objectives

To characterize the phenotype and genotype of GM2‐gangliosidosis disease in an affected dog.

Animals

One affected Shiba Inu and a clinically healthy dog.

Methods

Clinical and neurologic evaluation, brain magnetic resonance imaging (MRI), assays of lysosomal enzyme activities, and sequencing of all coding regions of HEXA, HEXB, and GM2A genes.

Results

A 14‐month‐old, female Shiba Inu presented with clinical signs resembling GM2‐gangliosidosis in humans and GM1‐gangliosidosis in the Shiba Inu. Magnetic resonance imaging (MRI) of the dog's brain indicated neurodegenerative disease, and evaluation of cerebrospinal fluid (CSF) identified storage granules in leukocytes. Lysosomal enzyme assays of plasma and leukocytes showed deficiencies of Hex‐A and Hex‐B activities in both tissues. Genetic analysis identified a homozygous, 3‐base pair deletion in the HEXB gene (c.618‐620delCCT).

Conclusions and Clinical Importance

Clinical, biochemical, and molecular features are characterized in a Shiba Inu with GM2‐gangliosidosis. The deletion of 3 adjacent base pairs in HEXB predicts the loss of a leucine residue at amino acid position 207 (p.Leu207del) supporting the hypothesis that GM2‐gangliosidosis seen in this dog is the Sandhoff type. Because GM1‐gangliosidosis also exists in this breed with almost identical clinical signs, genetic testing for both GM1‐ and GM2‐gangliosidosis should be considered to make a definitive diagnosis.     

一种基于硼氢化钠的新型储氢材料研究

硼氢化钠的水解产物是一种新的储氢材料，其良好的可逆储氢能力在5MPa氢气压力真空条件下3分钟后在室温下吸收氢150。根据报道，它已被证明，这两种改变的催化剂NaBH4水解和添加催化剂直接插入接口是两个非常有效进一步提高接口的储氢容量。对hpsb-y2o3脱氢TiO2分别掺杂均达到重量2.4%和重量4.6%。重要的是对hpsb-y2o3可逆的脱氢能力不下降后成功———超循环。相比之下，对hpsb-ceo2可逆脱氢高达到重量5.9%，在室温下5分钟后3MPa氢气压力150氢吸附。     

Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

南方地区高水分玉米安全度夏技术探讨

根据水分的高低,对新收购的高水分玉米分别储存在露天囤和高大平房仓中,将露天囤里的玉米通风降水后转入高大平房仓储存,再对玉米通风降温,并采取仓房密封、悬挂防晒网、空调控温等配套措施控制粮温,期间采取磷化铝多次补药熏蒸杀灭粮堆内害虫及霉菌,使玉米安全度夏。     

无瓣海桑种子特征及不同贮藏环境下萌发特性的研究

种子的萌发除了与自身的生理结构、萌发时外界条件有关外,不同的贮藏条件和时间处理对种子萌发也有影响.本实验以无瓣海桑种子为研究对象,通过无瓣海桑种子的基本特征及不同贮藏环境(常温、常温干燥、-4℃、-70℃分别对应Ⅰ、Ⅱ、Ⅲ、Ⅳ)和时间(15,30,45,60,90 d)下其种子萌发特性的分析发现:径级对无瓣海桑的果重和种子的粒数均有显著影响;低温处理可显著缩短种子的萌发时间,同时有助于保持无瓣海桑的种子活性;无瓣海桑种子的活力会随着贮藏时间的增加而下降,因此不适宜长期贮藏,但短期贮藏时低温干燥贮藏可较好地保持其种子活力.     

初探粮食仓储的管理预案

在现代新的形势下,粮食仓储的管理工作显然是非常重要的,对于保障粮食安全是非常重要的,因为不管是从哪方面考虑,它都占据着一个至关重要的位置。所以,做好粮食工作的关键之一就是要加强对粮食仓储的管理。