保温型刚性护面渠道设计

在刚性面层下设置一道性能良好的保温层来防止基土冻结,可以从根本上解决冻胀破坏的问题。这种带保温层的刚性护面渠道称为保温型刚性护面渠道,根据热工原理,提出了保温型刚性护面渠道横断面设计在构造方面的要求,进一步推导出计算保温层厚度的有关公式,并给出算例,还指出了需要进一步研究和探讨的问题。     

基于ANSYS的永磁电机转子冲片结构的优化设计

运用ANSYS的优化设计模块对永磁电机转子冲片隔磁桥附近的结构进行优化设计,采用APDL参数化设计通过拓扑形状优化功能确定冲片优化的初始区域,在此基础上运用尺寸优化功能确定最优的形状尺寸参数,最终结合工程化要求确定转子冲片的施工结构尺寸。电机最后通过最高转速试验表明文章运用的结构优化设计方法切实可行,满足工程化生产要求。     

Effect of Se and VE Supplement on Semen Quality,Antioxidant Enzyme Activities and Heat Shock Protein Expression of Goat in High Temperature Season

This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality, antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4 groups, fed with basal diet(control group), basal diet+0.5 mg/kg Se(Se group), basal diet+100 mg/kg VE(VE group), and basal diet+0.5 mg/kg Se+100 mg/kg VE(Se+VE group), respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality, antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group, the ejaculate volume was not significantly affected by Se or VE supplement(P>0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P<0.05), and the abnormal rate was decreased extremely significantly(P<0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P<0.01), SOD(P<0.05), CAT(P<0.05) and T-AOC(P<0.01), and lower MDA concentration in seminal plasma(P<0.05).The relative expression levels of HSP70 and HSP90 mRNA in supplement groups were decreased extremely significantly(P<0.01).However, there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion, the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density, sperm motility, the antioxidant enzyme activities, and decreasing the abnormal rate in hot season of Hainan.Finally, Se and VE supplement had good effects on relieving the environment heat stress.     

Assessment of interactions between glutamine and glucose on meat quality,AMPK, and glutamine concentrations in pectoralis major meat of broilers under acute heat stress

The objective of this experiment was to evaluate the effects of dietary glutamine and glucose on pectoralis major meat quality in broilers under acute heat stress. Three hundred 35-day-old male broilers (Arbor Acres) were randomly divided into 5 groups. Broilers in the positive control group were kept in a thermoneutral environment (23 ± 1°C, RH 45%–55%) and fed a basic diet, whereas the experimental (2 × 2) group was exposed to acute heat stress (34 ± 1°C, RH 65%–75%) for 12 h and fed the basic diet that contained glutamine (0 and 10 g/kg) and glucose (0 and 10 g/kg). Compared with the positive control group, acute heat stress without glutamine and glucose supplementation increased (P < 0.05) drip loss, L* values, and AMPK concentrations, but it decreased (P < 0.05) water-holding capacity, moisture, pH, a* values, b* values, and glutamine concentrations in pectoralis major meat of chickens. However, dietary glutamine (10 g/kg) increased (P < 0.05) water-holding capacity, moisture, pH, a* values, b* values, and glutamine concentrations, while it decreased (P < 0.05) drip loss, L* values, and AMPK concentrations in heat-stressed chicken pectoralis major meat. Dietary glucose (10 g/kg) increased (P < 0.05) water-holding capacity, moisture, pH, and a* and b* values, while it decreased (P < 0.05) L* values and AMPK concentrations of pectoralis major meat in chickens exposed to acute high temperature. There were significant interactions between glutamine and glucose in the water-holding capacity, pH values, AMPK, and glutamine levels of pectoralis major meat in the heat-stressed chickens. The above results suggest that the addition of glutamine and glucose in the diet is necessary for broilers during acute heat stress condition.     

Molecular cloning,purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.     

抗青枯病樱桃番茄品种的初步筛选及评价

对28个樱桃番茄品种的田间抗病性、植株类型、单果质量、果色、可溶性固形物含量等主要经济性状进行调查。试验结果表明,感病品种红艳发病最重,发病率为73.67%,其次是红箭、红日和金艳,发病率分别为23.33%、21.67%和20.33%,其他品种发病率都在20%以下;28个品种的果实颜色、大小、口感等差异明显,除黑宝石映泰及黄金蛋可溶性固形物含量在4.10%以下外,其他品种可溶性固形物含量都达5.20%以上。综合分析,筛选出绿天使、粉仙、红娘9号、绿圆、格丽贝、红椭圆等11个抗青枯病且经济性状优良的樱桃番茄品种,为优质抗青枯病樱桃番茄的选育和推广提供了参考。     

多梯度氮磷添加对高寒草甸植物群落生物量与氮磷含量的影响

为研究不同梯度氮、磷单独及混合添加对高寒草甸植物群落生物量、植物养分含量及化学计量比的影响,本实验分析了青海省门源县典型高寒草甸植物群落地上总生物量,功能群水平地上生物量,植物全氮、全磷含量及氮磷比对多梯度氮、磷添加的响应情况。结果表明：氮、磷添加均对群落地上生物量影响极显著(P<0.001);氮添加对禾草类和豆科生物量影响极显著(P<0.001);磷添加对禾草类和莎草类生物量影响极显著(P<0.001);氮磷交互作用对豆科和莎草类生物量影响显著(P<0.05)。群落水平上,氮添加显著提高了植物全氮含量,对植物氮磷比(N∶P)有正效应,磷添加显著提高了植物全磷含量,对植物N∶P有负效应,植物全磷对N∶P的负效应大于全氮对N∶P的正效应。本研究表明氮、磷添加可能会使高寒草甸植物群落组成和植物养分含量发生改变,植物群落逐渐向禾草类发展;此外,高寒草甸植物生长趋向于受氮磷共同限制。     

Nonlinear Characteristic Modeling of Servo Value on Hydraulic Bending Roll System''s Dynamic Characteristic

Nonlinear characteristic of servo value is the important factor influencing hydraulic system, the model of electro-hydraulic servo pressure control system is designed. In this model, flux-equation of servo valve is specially applied and some non-linear characteristics are considered. Furthermore, SIMULINK toolbox of MATLAB language software is applied to solve mathematic model and simulation curves of some variables are obtained. The calculation results are in good agreement with the experimental results.