根瘤菌参比菌株23S rRNA基因数量和定位及其系统发育群

    为了明确23S rRNA基因数量和定位是否可更好地揭示根瘤菌参比菌株的系统发育关系,采用I-CeuI酶切和脉冲场凝胶电泳(PFGE)结合的方法,对根瘤菌株23S rRNA基因的数量和定位进行分析,并依据其相似性进行聚群.结果显示.根瘤菌参比菌株可聚为19个系统发育群.其中,在属的水平上,13个系统发育群与现行分类群(不包括依据16S rRNA基因序列分析结果)一致,6个不一致.在种的水平上,现行分类群中同种的根瘤菌可进一步细分为不同的系统发育群.这表明:I-CeuI酶切和PFGE结合的方法能从基因组特征角度对根瘤菌参比菌株进行更加细化的系统发育分类,并使其属种间的同质性更好.     

基于软件体系结构的高校仪器设备采购管理系统设计与开发

本文通过对高校仪器设备采购工作流程的分析,利用软件体系结构的理论,提出了基于B/S、C/S体系结构的仪器设备采购管理系统设计、总体结构和功能实现方法.     

Analysis of introgression of the Tulipa fosteriana genome into Tulipa gesneriana using GISH and FISH

Southern hybridization and genomic in situ hybridization (GISH) have demonstrated that ‘Purissima’ (2n = 2x = 24) is an interspecific hybrid comprised of one genome of Tulipa (T.) gesneriana and one genome of T. fosteriana. Backcrossing T. gesneriana with ‘Purissima’ was partially successful. Simultaneous GISH and fluorescence in situ hybridization (FISH) distinguished chromosomes from both parent genomes, as well as recombinant chromosomes, in interspecific hybrids and their progeny. Chromosome recombination was observed in all cultivars except ‘Purissima’ and ‘Kouki’ (2n = 3x = 36). ‘Kouki’ (2n = 3x = 36) had two genomes of the T. gesneriana and a single genome of the T. fosteriana. The number of nonrecombinant T. fosteriana chromosomes in ‘Judith Leyster’ (2n = 4x = 48) and ‘Purissima’ progeny varied from two in ‘Hatsuzakura’ to six in ‘Kikomachi’ and ‘Momotaro’. The number and type of recombinant chromosomes also differed among cultivars. The total number of translocations ranged from one in ‘Kikomachi’ to six in ‘Hatsuzakura’. Each was a combination of a single T. fosteriana fragment and a single T. gesneriana fragment, indicating that they resulted from a single crossover event. Sequential GISH and FISH analysis with rDNA probes yielded chromosome-specific markers that were used to identify most of the chromosomes in ‘Purissima’ progeny. This is the first report of introgression of T. fosteriana chromatin into the T. gesneriana genome.     

基于R/S分析的参考作物腾发量时间序列分形特征

近年来分形理论在水文、股票及气象等行业中应用较多,在时间序列分析的非线性特征方面展示出强大魅力。采用重标极差方法分析西部地区6个站ETo时间序列的变化趋势和分形特征,通过对比发现:①所研究各站气象要素及ETo年变化趋势比较表明,所有站相对湿度均呈降低趋势,年积温均呈增加趋势,日照时数均呈降低趋势,年ETo值总体也呈降低趋势。②年尺度下各站点Hurst指数均大于0.5,同时分维数D均小于1.5,意味着ETo年序列在将来一段时间仍然保持与过去相一致的变化趋势,也即未来的总体趋势仍为减少趋势,各地区ETo年序列具有持续性。③各站点各月Hurst指数同样都大于0.5,分维数D均小于1.5,意味着1~12月ETo序列在将来一段时间仍然保持与过去相一致的变化趋势。此外,各站点年Hurst指数均大于月Hurst指数,表明年ETo序列比月ETo序列具有更长时间的持续性。     

合肥市紫外线指数预报业务系统的分析和研究

介绍了合肥地区紫外线预报系统的建立依据以及功能。由于紫外线和太阳高度角、云量等要素之间有很好的相关性,利用2004~2006年紫外线观测资料和同期气象资料,运用统计学方法建立紫外线预报方程,并根据预报方程建立一个集资料采集、信息查询、预报制作、产品发布、定量评分于一体的具有较好的人机交互的紫外线预报业务系统。     

基于S3C2410的原木形状识别机控制系统设计

介绍一种基于S3C2410的原木形状识别机控制系统的软硬件设计.采用32位ARM9内核的S3C2410作为系统控制的CPU,操作系统为嵌入式ARM-LINUX,利用Qt/E设计用户界面,构成软硬件可剪裁的原木形状识别机嵌入式控制系统.该系统具有体积小、开发资金投入少等特点,可对原木几何特征进行高速精确识别和仿真,提高生产效率.     

3S的集成与发展及其在林业中的应用

本文在总结RS、GIS和GPS及其特点的基础上,对3S的集成与发展进行了介绍。指出3S集成技术是当今空间科学领域的一个研究前沿,3S技术已广泛应用于各个领域;多波段、多平台的RS信息及其在野外调查中所必须的GPS和多功能GIS的结合使用,在林业中的应用也具有广阔前景。     

5个罗非鱼品种(系)线粒体DNA 16S rRNA和Cyt b基因片段序列分析

对3个尼罗罗非鱼(Oreochromis niloticus)品系(埃及、无锡、吉富)和奥利亚罗非鱼(Oreochromis au-reus)以及杂交种奥吉(奥利亚♀×吉富♂)的线粒体16SrRNA和细胞色素b(Cytb)的部分序列进行了PCR扩增和序列测定,并分析了其序列差异。结果表明:PCR产物纯化后测序,16S rRNA扩增产物得到长度为596bp的基因片段,其碱基组成G+C平均含量为47.5%,序列比对分析显示变异位点13个,没有碱基的插入/缺失变异,转换/颠换比率为3.33;Cytb扩增产物测序得到659bp的同源片段,其碱基组成G+C含量平均为45.2%,无插入/缺失变异,变异位点共2个,都为转换,2处变异都发生在密码子第3位上,编码的氨基酸未发生变异。序列分析表明,尼罗罗非鱼3品系的序列完全相同,奥利亚的序列则与奥吉相同。这表明在罗非鱼线粒体DNA中,16S rRNA进化速率相对较快,而Cytb基因则高度保守,不适于研究罗非鱼种内和种间的亲缘关系和种类鉴别标记。     

Cytological study and PCD assay on pollen development of photoperiod sensitive genic male sterile rice

A systematic cytological comparison of the anther development of photoperiod sensitive genic male sterile (PSGMS) rice with its normal fertility counterpart was conducted. The results showed that pollen abortion in PSGMS rice occurred first no later than the pollen mother cell (PMC) stage and continued during the entire process of pollen development till pollen degradation. This abortive process was closely associated with the abnormal behavior of tapetum. Although tapetum degeneration in the PSGMS rice initiated as early as at the PMC stage, it proceeded slowly and did not complete until the breakdown of the pollen, in sharp contrast to the rapid disintegration of the tapetal layer during the late microspore to the bicellular pollen stage in the fertile rice. Such cytological observation was supported by the results of the TUNEL (TdT2 mediated dU TP Nick End Labeling) assay that detectsDNA fragmentation resulting from programmed cell death (PCD), indicating that the tapetum degeneration occurs in the process of PCD. __________ Translated from Journal of Huazhong Agricultural University, 2007, 26(3): 283–288 [译自: 华中农业大学学报]     

新疆平原区地下水资源评价及其结果分析

集成3S对地球科学信息的处理与分析功能,结合VBA编程技术实现了新疆地下水资源评价计算方法的程序化和计算步骤的自动化。该评价模式为类似的地下水资源评价提供了参考,可推广应用于针对新疆某个流域或西北干旱区的地下水资源评价。新疆地下水资源评价主要结果为,总计算面积120.82×104km2,其中平原区计算面积50.91×104km2。平原区地下水多年平均总补给量337.6×108m3,总排泄量340.5×108m3。平原灌区现状开采地下水量56.4×108m3,近期和中期评价地下水可开采量约153.2×108m3。