FR 02全长基因的克隆表达载体的构建及转化苹果

从铁胁迫诱导的拟南芥植株根中克隆了全长的FR02（Fe^3＋-螯合物还原酶）基因,并将FR02基因构建到了植物高效表达载体pCB302中。通过农杆菌介导的转化和PCR及Southern blot检测证明FR02基因正向的插入了苹果基因组中。以破坏生长点的茎尖为外植体的苹果基因转化方法转化率高达10．8％。FR02全长基因的成功克隆、构建和转化为高铁、抗黄化苹果和其他高铁作物新品种的培育开辟了一条新路。     

桂糖31号在合浦种性表现及蔗农成本收益

桂糖31号（原代号：桂糖02/281）,亲系：粤糖85-177×CP81-1254,由广西农科院甘蔗研究所选育.2009-2010年广西甘蔗品种区域试验合浦试点结果表明：桂糖02/281分蘖多,宿根性好,宿根发株率高,中大茎,蔗茎产量高,比新台糖22号增产显著,平均含糖量为12895kg/hm2,比新台糖22增7.89％.蔗农投资种植桂糖31号总成本35949.71元/hm2,比新台糖22增15.84％.土地净产值为17179.25元/hm2,比新台糖22增15.78％.土地纯收益为8533.61元/hm2,比新台糖22增37.82％.原料蔗成本价格为384.73元/t,比新台糖22减少3.72％.劳动力净产值为每一劳动日75.35元,比新台糖22增15.78％.资金投入纯收益率为23.74元/100元,比新台糖22增18.97％.     

Length of cuttings in juvenile development of a hybrid poplar clone

The influence of length of cuttings on establishment of a short rotation plantation was investigated over a period of two years using direct field planting of unrooted Populus 02x84h070137554/xxlarge8220.gif" alt="ldquo" align="MIDDLE" BORDER="0">Rasumowskiana02x84h070137554/xxlarge8221.gif" alt="rdquo" align="MIDDLE" BORDER="0"> cuttings. The longer the cuttings, the higher were the survival and dry mass production. Survival at the end of the second growing season varied from 75% to 97%. In two years, the two longest cutting lengths (40 cm and 50 cm) produced about 100 g dry mass per living cutting; but owing to the higher survival rate of the longest cuttings, there was a difference per area unit: 360 g/m² for 40 cm and 400 g/m² for 50 cm-long cuttings. The shortest (10 cm) cuttings produced only 115 g/m².     

FJ-MA-02引诱剂林间松墨天牛引诱效果及活虫捕捉器的研制

研制开发的FJ-Ma-0 2松墨天牛引诱剂 ,在福州国家森林公园低密度虫口的松阔混交林内 ,当年新诱捕点每个诱捕器平均引诱到 1 1 9 3头松墨天牛 ,其中 46 48%为雌成虫 ,平均每头雌虫怀卵量为 3 7 82粒。在福建中部地区诱虫高峰时间为 5— 8月。在诱虫点周围翌年虫口数量可降低 2 3。研制的活虫捕捉器 ,通过简单原理可直接捕捉活虫 ,其活虫可供各种试验使用。     

Response of British lichens to climate change scenarios: Trends and uncertainties in the projected impact for contrasting biogeographic groups

Projection of species-response to climate change scenarios is a key tool in conservation strategy. Previous studies have projected climate change impacts for animal and vascular plant species using the ‘bioclimatic envelope’ approach. In this study we apply the principles of the bioclimatic envelope approach to examine the response of 26 lichen species whose distributions are well characterised within the British Isles. Lichen species were subjectively selected based on their contrasting distributions, and their ecological traits, which fulfilled as closely as possible the assumptions of the bioclimatic envelope method. We used a split-sampling approach to model the species-response to present-day climate using confirmed records and pseudo-absences as input data, and testing each model against an ‘independent’ calibration dataset. Predictive models were projected using standard climate change scenarios comprising the UKCIP02 data. Projections indicate broad trends in the response of species placed into contrasting biogeographic groups, and point to the potential for significant change in the spatial distribution of the British lichen flora. We highlight putative threats to montane and Boreal elements of the lichen flora, and emphasise significant uncertainty in projected response of the UK’s internationally important oceanic flora.     

转基因八楞海棠抗黄化鉴定研究

研究了转FRO2基因八楞海棠抗黄化性。结果显示,转FRO2基因八楞海棠植株的FRO2基因得到了过量表达;在缺铁胁迫下,转FRO2基因八楞海棠根系铁还原酶活性为对照的2.2倍;根系铁吸收能力和有效铁还原能力明显加强,叶片中有效铁含量是对照的2.1倍;转FRO2基因叶片颜色比对照绿,黄化程度减轻。在正常培养条件下,转FRO2基因八楞海棠根系的FRO2基因表达量增加,铁还原酶活性是对照的1.88倍,叶片有效铁含量是对照植株的1.7倍。转FRO2基因八楞海棠可以用于盐碱地及其缺铁土壤的生产,以解决苹果黄化问题。     

Numerical Simulation of Root Growth Dynamics of CO2-Enriched Hybrid Rice Cultivar Shanyou 63 Under Fully Open-Air Field,Conditions

Hybrid indica rice(Oryza sativa L.) cultivars play an important role in rice production system due to its heterosis,resistance to environmental stress,large panicle,and high yield potential.However,no attention has been given to its root growth dynamic responses to rising atmospheric CO2 concentration([CO2 ]) in conjunction with nitrogen(N) availability.Free air CO2 enrichment(FACE) and N have significant effects on rice root growth.In this experiment,a hybrid cultivar Shanyou 63(Oryza sativa L.) was used to study the effects of FACE and N levels on roots growth of rice.The results showed a significant increase in both adventitious root volume(ARV) and adventitious root dry weight(ARD) under the FACE treatment.The application of nitrogen also increased ARV and ARD,but the increase was smaller than that under FACE treatment.On the basis of the FACE experiment,numerical models for rice adventitious root volume and dry weight were built with the time as the driving factor.The models illustrated the dynamic development of rice adventitious root volume and dry weight after transplanting,regulated either by the influence factor of atmospheric [CO2 ] or by N application.The models were successfully used to predict ARV and ARD under FACE treatment in a different year with the predicted data being closely related to the actual experimental data.The model had guiding significance to growth regulation of rice root under the condition of atmospheric [CO2 ] rising in the future.     

黄曲霉毒素的细胞毒性及机制

以正常人肝细胞（L-02细胞）为研究对象,根据细胞增殖率、活性氧（reactive oxygen species,ROS）含量、丙二醛（malondialdehyde,MDA）含量等指标的变化研究黄曲霉毒素B1（aflatoxin B1,AFB1）的毒性作用及氧化应激损伤,选用VC作为AFB1损伤肝细胞的保护剂,通过比色法测定细胞的相对存活率,从细胞周期的变化和细胞凋亡率研究AFB1引起L-02细胞凋亡的程度及机制。结果表明：根据AFB1的半数细胞抑制率（inhibition of cell,IC）（IC     

基于FLAASH的CBERS-02B星CCD影像大气校正

中巴资源卫星的CCD数据作为我国自主卫星数据源,在遥感应用方面发挥着重要作用。但由于CCD数据所获取的可见光-近红外波段信息易受大气因素干扰,因此寻求一种适合于中巴资源卫星CCD数据快速、有效的大气校正方法,以提高影像的利用率。介绍FLAASH大气校正的主要算法原理,并利用其进行CBERS-02B星CCD影像的大气纠正试验。通过分析校正前后的影像目视效果、典型地物光谱特征和NDVI值,表明FLAASH方法能够快速、有效地纠正大气因素对CBERS-02B星CCD影像的影响,对于植被覆盖地区纠正效果尤为明显。因此,FLAASH大气校正方法能够为大数据量的CBERS系列卫星CCD数据的大气校正提供了快速、可行及有效的方法。