Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province

To investigate the epidemic situation of H6N6 subtype avian influenza virus (AIV) in Guizhou province,A/duck/Guizhou/013/2014 was isolated from Sansui duck in live poultry market of Guizhou in 2014,the hemagglutinin (HA) and neuraminidase (NA) genes of DK/GZ/14 were subjected to clone and sequence analysis.The results showed that HA gene had the highest nucleotide homologies (97.5%) with the duck-origin H6N6 subtype AIV isolated from Eastern China in 2009,and the strains of HA gene proteolytic cleavage sites was P-Q-I-E-T-R-G,which accordeol with the molecular characteristic of low pathogenic AIV (LPAIV).However,NA gene of A/duck/Guizhou/013/2014 had the highest nucleotide homologies (98.2%) with the duck-origin H6N6 subtype AIV isolated from Fujian in 2007.The phylogenetic tree showed that A/duck/Guizhou/013/2014 and Hunan strains located in the same branch,while three duck-origin H6N6 subtype AIV isolated from Guizhou in 2007 and A/duck/Guizhou/013/2014 located in the different branch for HA and NA genes in genetic evolution,which suggested that A/duck/Guizhou/013/2014 was far with the local H6N6 subtype.The results also clearly indicated that duck-origin H6N6 subtype AIV had genetic diversity in duck population in Guizhou.     

Research Lactobacillus rhamnosusFermented Herbal and Bacillus subtilis Complex on Immune Performance,E.coli Infection of Broiler

This experiment was designed to study the complex of Lactobacillus rhamnosus fermented herbal and Bacillus subtilis on White Feather broiler immunity performance and impact of Escherichia coli infection.360 one-day-old broiler chickens were randomly divided into 3 groups with 4 replicates in each group and 30 chickens per replicate.The pretrial period lasted for 7 d,and the experiment lasted for 35 d.The chickens in the group Ⅰ(positive control group) and group Ⅱ(negative control group)were all only fed a basal diet,group Ⅲ was test group,by additive 1% fermented herbal preparations,groups Ⅱ and Ⅲ were intraperitoneal injection of 1 mL E.coli at 35 d,broiler mortality,immune organ index,cecalmicroflora content,immunoglobulin levels,IL-2 and IL-6 content were tested.The results showed that injection of E.coli caused massive death of chickens,group Ⅱtook up to 75.00%,it was significantly higher than groups Ⅰ and Ⅲ (P < 0.05),the mortality in group Ⅲ was significantly lower than group Ⅱ(P < 0.05),was only 23.33%.Injection of E.coli maked spleen index and thymus index of group Ⅱincreased significantly (P < 0.05),the spleen index and thymus index of groups Ⅰ and Ⅲ were no significant difference (P > 0.05).E.coli counts was significantly decreased after injectionin group Ⅲ (P < 0.05),but the number of intestinal Lactobacilli of group Ⅲ was significantly increased (P < 0.05),and inhibited the propagation of E.coli,the counts of E.coli in groups Ⅰ and Ⅲ were no significant difference (P > 0.05).At 42 d,the sIgA of the intestinal fluid in group Ⅲ were higher than that of groups Ⅰ and Ⅱ with 11.99% and 36.56%,respectively(P < 0.05).The serum IgG concentrations of group Ⅲ was higher than that of groupsⅠand Ⅱwith 14.68% and 28.15%,respectively(P < 0.05).At 42 d,the IL-2 content of group Ⅱ was the lowest,it was significantly lower than group Ⅲ(P < 0.05),the IL-6 of group Ⅲ was significantly lower than group Ⅱ(P < 0.05).     

两种口蹄疫抗体检测方法的操作体会

正免疫抗体效价检测,已成为现代动物疫病防控科学评价动物免疫接种效果的有效方法之一。正向间接血凝试验通常只用于O型口蹄疫抗体检测,该方法主要利用口蹄疫抗原来检测血清中是否含有口蹄疫抗体或判定抗体滴度能否达到免疫保护标准。液相阻断ELISA适用于O型、亚洲I型和A型口蹄疫抗体检测,其操作较正向间接血凝试验相对繁琐,但该方法精准性和稳定性高,既能评价口蹄疫免疫情况,又能检测口蹄疫病毒     

鸭瘟的病原、临床表现与防控措施

鸭瘟也叫作鸭病毒性肠炎,还可称为大头瘟,是一种急性、热性、败血性传染病,主要是鸭、鹅以及多种雁形目的动物容易发生。该病具有较高的致死率,对养鸭业发展造成严重危害。本文组要对该病的病原、流行病学、临床症状进行分析,并介绍疫苗免疫和发病后的处理措施,供大家参考。     

母猪群猪瘟疫苗跟胎免疫与普免对猪瘟抗体水平的影响

母猪猪瘟疫苗的免疫方式分为跟胎和普免2种。为了比较2种免疫方式接种后母猪在一个生产繁殖周期内抗体水平的变化情况,本研究检测了6个饲养管理很好的猪场的猪瘟抗体水平。对其中跟胎免疫的4个母猪群在怀孕前期、怀孕中期、怀孕后期和哺乳期等4个阶段的抗体水平分别进行了检测,同时,对4个猪群的仔猪的母源抗体水平也进行了检测。对一个普免的猪群分别在其免疫后第1月、2月、3月和4月分别跟踪检测母猪猪瘟抗体。另外,分析了一个普免的猪群4年来的检测数据。结果发现不管是跟胎免疫还是普免,免疫水平好的猪群在一个生产周期内均可保持很高的抗体水平。而母猪群即使抗体水平很高且整齐度好,仔猪的母源抗体水平也难以保持较好的整齐度。     

猪口蹄疫O型灭活疫苗不同组合免疫效果

试验主要通过仔猪口蹄疫母源抗体消减规律来确定仔猪口蹄疫O型灭活疫苗首免日龄,选择不同猪口蹄疫O型灭活疫苗进行组合免疫接种后,观察其免疫抗体效价水平变化,从而评价不同疫苗组合的免疫效果,对养猪生产指导具有重要意义。     

猪蓝耳病活疫苗的使用误区及正确方法

正猪繁殖与呼吸综合征俗称"猪蓝耳病"。以母猪发热、不食,妊娠母猪早产、流产、产死胎等为特征,10日龄以内小猪死亡率达70%,断奶后仔猪表现发热、呼吸困难,个别猪耳部和腹侧皮肤呈蓝紫色,失去运动能力,免疫力下降等。有很多养殖老板对接种猪蓝耳病疫苗已经产生了恐惧,因为现在生产疫苗的厂家很多。有多数猪场已经接种过猪蓝耳病疫苗,后期依然发病,特别是500头以下的猪场,经过近3年的走访,     

猪支原体肺炎疫苗的临床免疫意义

猪支原体肺炎呈世界性分布,严重危害养猪业的发展,多数养殖户朋友对猪支原体肺炎的理解停留在“咳”、“喘”两个方面,加之单纯的猪支原体肺炎往往不会导致猪只死亡,引不起大家的重视,笔者结合临床经验跟读者分享一下猪支原体肺炎疫苗的临床免疫意义。     

农村动物免疫存在的问题及对策

随着我国经济发展步伐加快,畜牧业得到了长足发展,但与此同时像高致病性禽流感、口蹄疫等动物疫病也频繁发生,动物防疫工作已经受到各级政府的高度重视,领导力度明显加大,防疫基础设施得到改善,免疫密度明显提高,防疫成效十分显著.但是,从当前农村动物防疫工作实际情况来看,部分地方仍然存在着免疫死角,有的地方免疫效果较差,这给重大动物疫病的发生留下了重大隐患,给当前我国农村动物防疫工作带来了挑战.笔者结合自身基层工作实践,试对新形势下农村动物免疫工作存在的问题加以剖析.     

规模化猪场猪瘟疫苗群体免疫程序的研究

CSF是危害我国养猪业最严重的疫病之一。由于我国实行了猪瘟强制免疫政策使得猪瘟疫情总体上得到了控制。但是近年来免疫程序不合理等因素的给各猪场带来极大的风险。本文采用数据统计法对猪瘟疫苗免疫程序后的结果进行对比分析,根据猪体内的抗体比率优化了免疫程序,为规模猪场的防疫技术提供了参考。