Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

Halbach磁阵多浮体海波发电结构功率响应研究

以Halbach磁阵多浮体海波发电结构为对象,研究了其在垂荡运动下的能量转换特性。基于线性波理论,建立了线圈浮筒的运动方程和能量转换模型,数值模拟了结构阻尼系数、弹簧刚度系数以及线圈浮筒的长度和半径,此外,数值还模拟了吃水对海波发电结构平均能量转换功率及共振频率的影响。结果表明:根据不同的海波状况,合理设计了海波发电结构的参数,将有助于其平均能量转换功率的提高。     

吉林省降水振荡与地震的响应

大气降水是人们所熟知的一种自然现象,但降水何以能引起人们控制地震的现象呢,无疑是受到现代勘探技术证实,说明至少在大陆内部水是可能运移至相当深度而不被汽化的。本文特以吉林省为例,再次证明这种制约关系的存在和其对未来地震预报的实际意义。     

响应曲面法优化草莓汁花色苷促溶技术的研究

为了加速溶出草莓汁中的花青毒,以哈尼草莓为试验材料,原花青素含量为检测指标,依据中心组合响应面设计,研究果胶酶处理对草莓中色素物质溶出的影响。结果表明:果胶酶处理的最佳工艺条件为果胶酶添加浓度为(质量比)1.70‰,45℃处理2.87h;方差分析结果表明模型高度显著(P=0.0002),失拟性不显著(P0.1)。该模型可进行草莓汁加工中花青素溶出的分析及预测。通过该技术处理,可以得到花青素含量高、颜色较好的草莓汁。