论电子阅览室的知识导航与信息服务

本文立足于知识导航与信息服务，对网络环境下电子阅览室的服务内容、服务保障、目前面临的现状进行了论述，并提出了服务对策。     

过程性考核在《细胞生物学》课程教学中的应用

过程性考核是在课程实施的过程中对学生的学习进行评价的方式,强调对学生学习综合知识与能力素养的考核,能够克服一次性期末闭卷考试的不足.细胞生物学课程过程性考核根据教学内容分阶段采取多样化的考核方式,不仅可以促进学生分阶段完成学习任务,使任课教师及时了解学生的学习效果,而且还具有激发学生的学习主动性、引领学生自主学习等作用...     

Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method

In chickens, primordial germ cells (PGCs) are effective targets for advanced genome editing, including gene knock-in. Although a long-term culture system has been established for chicken PGCs, it is necessary to select a gene-editing tool that is efficient and precise for editing the PGC genome while maintaining its ability to contribute to the reproductive system. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and CRISPR-mediated precise integration into the target chromosome (CRIS-PITCh) methods are superior as the donor vector is easier to construct, has high genome editing efficiency, and does not select target cells, compared to the homologous recombination method, which has been conventionally used to generate knock-in chickens. In this study, we engineered knock-in chicken PGCs by integrating a fluorescent protein gene cassette as a fusion protein into the chicken vasa homolog (CVH) locus of chicken PGCs using the CRIS-PITCh method. The knock-in PGCs expressed the fluorescent protein in vitro and in vivo, facilitating the tracking of PGCs. Furthermore, we characterized the efficiency of engineering double knock-in cell lines. Knock-in cell clones were obtained by limiting dilution, and the efficiency of engineering double knock-in cell lines was confirmed by genotyping. We found that 82% of the analyzed clones were successfully knocked-in into both alleles. We suggest that the production of model chicken from the knock-in PGCs can contribute to various studies, such as the elucidation of the fate of germ cells and sex determination in chicken.     

畜禽精准饲喂管理技术发展现状与展望

传统的畜禽饲喂方式易产生畜禽营养不均、饲料浪费等问题,且人工劳动强度较大、生产率有待提高.随着信息技术的进步,以节省饲料和人工成本、促进畜禽营养均衡、保障畜产品品质及安全等为显著优势的精准饲喂管理技术应运而生,并逐渐研发应用.该文阐述了畜禽精准饲喂中饲养信息获取、饲料精准配方及智能饲喂技术的发展现状并分析了当前存在的问...     

探讨集中式排种器的排种机理及其发展

目前传统排种器多属于“一器单行”型式。机械式对种子尺寸要求严格、破碎率高;气力式能耗大、结构复杂、使用要求高。而集中式排种器(或系统)是对“一器单行”技术的突破,满足精密播种的要求。主要论述了国内外集中式排种器的工作原理、发展概况及其存在的问题。     

村民自治组织参与灾后重建的现状分析——以四川地震灾区为例

四川汶川5·12特大地震,导致四川省88个县（区）受灾,其中农村受灾尤为惨重。目前,灾后重建工作正在有序开展,农民既是灾后重建的主要力量,又是直接受益者,灾后重建必须发挥农民的主体作用,而村民自治是发挥农民主体作用的重要制度保障。对村民自治组织参与灾后重建的状况进行了实地调查,并剖析了制约村民自治组织作用发挥的主要因素,在此基础上提出了可行的对策建议。     

基于STM32的植株缺素水肥调节系统的设计与试验

设计了基于STM32单片机的水肥调节系统,该系统通过控制不同电磁阀开度实现不同元素水肥配比的调节。以生菜为例研究在其他元素水肥配比正常浓度的情况下,调节控制钾肥浓度的电磁阀开度,使得不同生菜对照组在具有不同钾肥浓度下生长,对比研究不同钾肥浓度与生菜叶片面积和植株高度的关系,从而得到电磁阀开度调整策略,实现水肥配比的精准调节。      

一种用于香蕉叶斑病防治的新型施药技术

试验一种将内吸性农药稀释后灌注于香蕉叶柄基部防治香蕉叶斑病的新型精确定量施药技术。结果表明,这种施药技术与常规施药技术相比,防效相当,且对香蕉的生长无不良影响,而农药成本仅为常规防治的18%,农药利用率提高4倍,值得推广应用。     

基于Kalman滤波农用车辆导航定位方法

因RTD-GPS定位精度不能满足农田导航作业的需要,研究了一种提高农用车辆自动导航定位精度的方法.建立天线补偿模型,对GPS天线晃动引起的误差进行了补偿;建立基于Kalman滤波模型,融合多传感器信息;使用自主开发的基于VRS的GPS接收机,作为RTD-GPS.将RTD-GPS、电子罗盘以及速度传感器获得信息进行Kalman滤波,其结果和高精度GPS数据进行了比较.实验证明,直线跟踪中,平均偏差由1.6019m减小到0.597m;曲线跟踪中,平均偏差由1.2085m减小到0.4861m.     

Path tracking in forest terrain by an autonomous forwarder

Abstract

Autonomous navigation in forest terrain, where operation paths are rarely straight or flat and obstacles are common, is challenging. This paper evaluates a system designed to autonomously follow previously demonstrated paths in a forest environment without loading/unloading timber, a pre-step in the development of fully autonomous forwarders. The system consisted of a forwarder equipped with a high-precision global positioning system to measure the vehicle's heading and position. A gyro was used to compensate for the influence of the vehicle's roll and pitch. On an ordinary clear-cut forest area with numerous stumps, the vehicle was able to follow two different tracks, three times each at a speed of 1 m s^?1, with a mean path tracking error of 6 and 7 cm, respectively. The error never exceeded 35 cm, and in 90% of the observations it was less than 14 and 15 cm, respectively. This accuracy is well within the necessary tolerance for forestry operations. In fact, a human operator would probably have a hard time following the track more accurately. Hence, the developed systems function satisfactorily when using previously demonstrated paths. However, further research on planning new paths in unknown unstructured terrain and on loading/unloading is required before timber transports can be fully automated.