甜菜吸收植酸能力及植酸酶效应研究

采用土培盆法进行了甜菜对外加有机磷－植酸的吸收利用特点和植酸酶对改善甜菜磷素营养及生产产量等效应的研究，结果表明：甜菜能在一定程度上吸收利用上土壤外源植酸态磷，其生产产量效果烛施等量无机磷的７０％以上；植酸酶的应用能显著加有机磷的水解，增加溶液中磷的含量，促进甜根系生长发育，提高磷吸收速度，增加磷的吸收量，并最终体现在生产产量的增加；植酸酶的效果随配施的植酸量的增加而增加，当配施的杆得达到６０ｍｇ     

闭式Leontief模型为可行的一个实用的判定程序

设Ｔ是某闭式Ｌｅｏｎｔｉｅｆ投入产出模型的投入矩阵，如果有正向量Ｘ使得Ｘ≥ＴＸ，则称该模型为可行的．本文给出闭式Ｌｅｏｎｔｉｅｆ模型是否可行的一个实用的判定程序．     

浅谈水利行业CAD制图及出图技术的几点认识

主要介绍了AUTOCAD绘图的基本方法及画图、出图及其他过程中应注意的问题，并提出了相应的应对措施。     

In vitro metabolism of ovarian steroids by bovine whole blood and plasma

Experiments were conducted to evaluate the effects of time and temperature on the potential of bovine whole blood (WB) or plasma (PL) to metabolize the ovarian steroids progesterone, estradiol-17β and testosterone. During a radioimmunoassay study (Experiment 1), we observed a temperature and time-dependent reduction (P<0.001) of plasma progesterone concentrations in samples incubated as WB at 5, 15, 25, or 35C for up to 48 hr. Most notable was the observation that 27% of progesterone present in controls was lost when WB was incubated at 5C for 48 hr and a 17% reduction was observed when PL samples were incubated at 35C for 48 hr. Immunoreactive estradiol-17β concentrations (Experiment 2) in PL and WB incubates were not affected by time or temperature. However, immunoreactive testosterone concentrations increased more than 3-fold by 48 hr in WB incubates held at 35C. To examine the latter observation further, ³H-progesteone was incubated with WB at 35C, followed by extraction and thin-layer chromatography (Experiment 3). Results generally supported RIA findings and revealed the presence of significant 17α-hydroxylase, 17–20 lyase and aromatase activity. Heretofore this has not been considered to occur outside major steroid metabolizing organs.     

引进开发推广适用农机具促进甜菜生产发展

介绍了包头华资实业股份有限公司在甜菜生产中引进、开发、推广的适用农机具及其应用效果,并提出了今后进一步发展的对策与建议。     

绵羊磷脂酶C-γ1对绵羊卵母细胞体外成熟的影响

卵母细胞成熟率可作为衡量体外培养卵母细胞质量和发育能力的指标。本研究旨在探讨PLC-γ1是否参与了绵羊卵母细胞的体外成熟及其影响。将绵羊卵母细胞在含有不同浓度的U73122（PLC抑制剂））及m-3M3FBS （PLC激活剂）的成熟培养液中进行培养,每个浓度150个细胞,重复试验3次,统计细胞成熟率、卵裂率及桑葚胚率以供筛选出PLC-γ1的最佳抑制及促进浓度。qPCR检测0.5 μmol·L^-1 U73122及0.5 μmol·L^-1 m-3M3FBS处理后48 h的卵母细胞中PLC-γ1、BAK、BAX、CASP3、CASP8、P53、BCL6基因mRNA水平表达情况,Western blot检测0.5 μmol·L^-1 U73122及0.5 μmol·L^-1 m-3M3FBS处理后48 h的卵母细胞中PLC-γ1、BAK、BAX、CASP3、CASP8、P53、BCL6蛋白的表达情况。每个浓度150个细胞,重复试验3次。在绵羊卵母细胞中,0.5 μmol·L^-1 U73122是促进成熟的最佳浓度,0.5 μmol·L^-1 m-3M3FBS是抑制成熟的最佳浓度。0.5 μmol·L^-1 U73122处理组卵裂率和囊胚率均显著低于对照组。0.5 μmol·L^-1 m-3M3FBS处理组卵裂率和囊胚率均显著高于对照组。通过qPCR检测结果显示,与对照组相比,U73122组中BAK、BAX、CASP3、CASP8、P53基因mRNA表达量显著上升,PLC-γ1、BCL6基因mRNA表达量显著下降;m-3M3FBS组则与之相反。Western blot结果显示,与对照组相比,U73122组中BAK、BAX、CASP8蛋白表达量显著增多,PLC-γ1、BCL6蛋白表达量显著减少,P53和CASP3蛋白表达量无明显变化。m-3M3FBS组中PLC-γ1、BCL6蛋白表达量显著增多,BAX、CASP3、P53蛋白表达量显著减少,BAK、CASP8蛋白表达量无明显变化。综上所述,本研究表明PLC-γ1在绵羊卵母细胞的体外成熟培养中发挥重要作用,其可调节卵母细胞的成熟以及早期胚胎的发育能力。     

抗丝状支原体丝状亚种单克隆抗体的制备及初步应用

旨在制备抗丝状支原体丝状亚种(Mmm)的特异性单克隆抗体(MAb),为牛传染性胸膜肺炎(CBPP)病原诊断的免疫学方法提供特异性抗体。本研究利用生物信息学技术分析了Mmm国内分离株Ben-1不同传代株的全基因组序列,选取M0071蛋白作为研究对象。将原核表达的可溶性重组蛋白M0071(rM0071)作为免疫原免疫BALB/c小鼠,通过有限稀释法和间接ELISA方法筛选得到能稳定分泌抗rM0071蛋白的单克隆抗体的杂交瘤细胞株。进一步制备单抗腹水并纯化,利用Western blot方法对该单抗进行特异性鉴定,同时测定其抗体效价和抗体亚类。随后利用间接免疫荧光试验(IFA)评价该单抗对细胞感染Mmm的检测能力。结果表明成功获得1株单克隆细胞株3C4A1,将其分泌抗体命名为MAb 3C4A1。特异性结果表明,MAb 3C4A1能与Mmm的分离株和标准株发生特异性反应,而不与山羊支原体山羊肺炎亚种、丝状支原体山羊亚种、牛鼻支原体、无乳支原体、牛支原体、leachii支原体和牛A型巴氏杆菌等发生反应。抗体亚类鉴定MAb 3C4A1属于IgG1亚类、轻链为κ链。经间接ELISA测定其抗体效价为1∶256 000。IFA试验结果表明,MAb 3C4A1仅与感染EBL细胞的Mmm发生绿色荧光反应,而与牛鼻支原体、无乳支原体、牛支原体感染的细胞不发生荧光反应,特异性良好。本研究制备的MAb 3C4A1具有良好的特异性和免疫反应性,可作为CBPP病原免疫学诊断的工具,为进一步研制CBPP病原鉴别诊断试剂盒提供了基础材料。     

利用不同砧木嫁接培育苹果苗的试验研究

通过几年大田试验，发现野生山定子经１年驯化后培育苹果苗可提高出圃率；人工实生山定子小苗根接培育苹果苗的出圃率可达８５％。利用剪下的主根段作砧嫁接培育苹果苗的出圃率可达５０％以上。     

The influences of boiling and drying treatments on the behaviors of tension wood with gelatinous layers in <Emphasis Type="Italic">Zelkova serrata</Emphasis>

This study examined how boiling and drying treatments influenced various physical properties of the tension wood with gelatinous fibers (G-fibers) of a 29-yearold Zelkova branch. By boiling treatment, tension wood with numerous G-fibers contracted considerably in the longitudinal direction and the longitudinal Young’s modulus decreased in spite of the water-saturated condition. The drying treatment caused green tension wood and boiled tension wood with numerous G-fibers to shrink longitudinally and increased their longitudinal Young’s moduli. These specific behaviors in tension wood were highly correlated with the proportion of G-fibers in a specimen and were probably caused by the microscopic behavior of cellulose microfibril (CMF) in the gelatinous layers (G-layers). The longitudinal shrinkage of tension wood due to drying suggests the existence of a hygro-sensible, noncrystalline region in the CMF, which is abundant in the G-layer. Furthermore, the noncrystalline region in the CMF softens during boiling treatment, resulting in the reduction of the longitudinal Young’s modulus in tension wood. The longitudinal contraction of tension wood with G-fibers by boiling might be caused by the tensile growth stress remaining in green G-layers. However, no changes were detected in the 004 d-spacing of cellulose crystal in tension wood from the boiling and drying treatments, regardless of the proportion of G-fibers.     

杉木萌芽纯林与混交林生长量及经济效益的研究

在杉木林采伐迹地上,通过不炼山、套种火力楠与深山含笑,进行杉木萌芽纯林与混交林的对比试验,9a试验结果表明:混交林中杉木的平均胸径、树高、单株材积、地上部分单株生物量分别比杉木萌芽纯林增加4 04%、8 75%、16 3%、1 43%;混交林中火力楠和深山含笑生长良好,混交林总蓄积量和乔木层地上部分总生物量分别为杉木纯林的1 47倍和1 32倍;混交林的投入产出比高于杉木萌芽纯林,说明本模式是恢复和提高杉木连栽地土壤生产力的可行方法。