长期饲喂高锌日粮对断奶仔猪免疫机能的影响

试验选用60头23日龄±1日龄临床检查健康的"杜×长×大"三元杂交断奶仔猪,按体重和性别随机分成3组,每组20头,分别饲喂基础日粮、基础日粮 3000mg/kg氧化锌、基础日粮 500mg/kg蛋氨酸锌,测定断奶仔猪免疫机能的变化。结果显示,在断奶后0d～21d饲喂高锌日粮能显著提高仔猪白细胞吞噬率和T淋巴细胞转化率,增加血清免疫球蛋白水平;而在断奶后42d、70d,仔猪白细胞吞噬率、T淋巴细胞转化和血清IgG、IgA水平显著下降。     

Experimental research on cardiac fibrosis induced by recombinant mouse interleukin-11 in mice

AIM To observe the effect of recombinant mouse interleukin-11 （rmIL-11）injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg^-1·d^-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P<0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P<0.05).Compared with control group, the CWI was increased (P<0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P<0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor.     

试论公共图书馆在"农家书屋"建设中的作用

"农家书屋"建设是我国公共文化服务建设的重大项目,是全国新闻出版服务社会主义新农村建设的重要举措.公共图书馆作为知识与信息的中心,公共文化服务的重要组成部分,应充分利用自身资源优势,主动与新闻出版部门合作,共同将"农家书屋"建设得更加规范、更具活力.     

水土保持林草措施生态服务功能价值化研究

水土保持林草措施具有诸多的生态功能,具体表现为对水源、土壤、固碳、净化、固沙、生物多样性发挥的积极作用.通过对2013年至2015年数据估算得出,我国水土保持林草措施所产生的生态服务功能价值为8318.79亿元.其中,保持与改良土壤价值占比最高.     

辅料和菌剂添加对牛粪堆肥腐熟的影响

为研究不同辅料与菌剂添加对牛粪堆肥过程中堆体理化性质的影响,试验以牛粪为原料,设置7个不同的辅料和菌剂处理, T1(牛粪+玉米秸秆+发酵菌剂)、T2(牛粪+稻草+发酵菌剂)、T3(牛粪+蘑菇菌渣+发酵菌剂)、T4(牛粪+玉米秸秆)、T5(牛粪+稻草)、T6(牛粪+蘑菇菌渣)、CK(牛粪不添加辅料和发酵菌剂)。结果表明,在堆肥过程中,各处理批次的水分含量和C/N均呈下降趋势,pH值先升后降,有机质先降后升,种子发芽指数随堆肥天数的增加而增加。T1和 T2处理堆体升温快、高温期持续时间长,最高温度可达到66℃~67℃,高温期持续30~32天,加速了堆肥腐熟进程,提高了种子发芽指数。堆肥结束时T1和T2处理的种子发芽指数分别达到78.26%和76.48%。隶属函数综合评价表明T1腐熟程度最高,即当牛粪与玉米秸秆配比为4:1时,添加发酵菌剂有利于堆肥腐熟。     

小叶锦鸡儿灌丛土壤肥力变化的研究

研究了内蒙古风蚀沙化区退化草原植被恢复或重建过程中灌丛对养分的聚集和土壤肥力的保护作用,结果表明灌丛大小和灌丛不同土层养分含量没有明显的变化趋势,灌丛内0~5 cm土层的养分含量高于相应的对照样地,5~20 cm土层有机质和速效氮含量高于相应的对照,灌丛土壤颗粒含量和本地区土壤本底颗粒组成有密切的关系,和对照区相比,短期内没有显著增加土壤的细粒成分,但灌丛在减少风蚀方面具有一定的作用。     

我国城市森林建设初探

基于城市森林的内涵和功能以及城市森林建设的理论和法律依据,结合我国目前城市森林建设现状及存在的问题,提出了我国城市森林建设的思路和对策。     

图书馆的文化力

作为文化机构。图书馆以其本身对文化的积淀,对文化的发展起到了推动作用。本文从图书馆的文化积累功能、文化整合功能、文化传播功能、文化教育功能四个方面阐述了图书馆的文化力。     

不同紫花苜蓿几个抗旱指标的灰色关联分析

运用灰色关联分析法,对5项抗旱指标与5个紫花苜蓿(Medicago Sativa)品种抗旱性的相关性进行了分析。关联度分析结果表明:各指标与苜蓿品种抗旱性的关联序为:游离脯氨酸含量>SOD>POD>相对含水量>叶片水势。各指标对苜蓿抗旱性的影响,以游离脯氨酸含量最大,其次为SOD及POD;再次相对含水量;而叶片水势的影响最小。     

金针菇单核体DAN3和M与其杂交双核体G1菌丝阶段基因表达差异的转录组初步分析

以金针菇(Flammulinavelutipes)单核菌株DAN3的基因组为参考,完成单核体DAN3和M及其杂交双核体G1在菌丝阶段的转录组测序与数据分析,比较两个样本间的差异基因,并对差异基因进行GO功能分析和KEGG pathway分析.结果表明:两个样本中共有显著性差异表达的基因86个,其中,在G1中呈上调、下调表达的基因数分别为41、45个,有2个基因在G1中特异表达.GO功能分析结果表明,86个差异基因中有40个基因比对上了GO功能注释,其中18个基因在G1中呈上调表达;单一生物过程和催化活性为显著性富集的功能,相关基因在G1中呈上调表达.KEGG pathway分析结果表明,22个差异基因被定位到17条Pathway,其中10个基因在G1中呈上调表达,包括赖氨酸代谢途径对应基因,3_M和G1菌丝样品中赖氨酸含量分别为1.70×103 ng/mg和1.06×103 ng/mg,说明G1中上调的基因可能与之降解相关;DNA复制是显著性富集的代谢途径,相关基因在G1中呈下调表达.