不同质量浓度2,4-D对硬粒小麦成熟胚愈伤组织诱导及植株再生的影响

以5种硬粒小麦成熟胚为外植体,研究了其在9种不同诱导培养基、2种不同分化培养基上的出愈率、诱导愈伤组织质量及分化率的差异.结果表明:在小麦愈伤组织的诱导阶段,当2,4 D的质量浓度在2.5～4.0mg·L-1时,5种小麦的愈伤组织均较好;在小麦愈伤组织的分化阶段,2,4 D质量浓度的变化对不同小麦愈伤组织分化的影响效果不同,但缺乏规律性;小麦的出愈率与愈伤组织质量无明显关系.     

Seasonal changes in the crude protein concentration of mixed swards of white clover/perennial ryegrass grown without fertilizer N in an organic farming system in the United Kingdom

Changes in the crude protein (CP) concentration of white clover and perennial ryegrass herbage from a mixed sward were determined on six sampling dates from May to October in each of 2 years. The swards were grown without fertilizer N in an organic farming system and continuously grazed by dairy cows during the grazing season. The annual mean contents of white clover in the dry matter (DM) of the sward were 272·3 and 307·0 g kg⁻¹ in Years 1 and 2. The mean CP concentrations of the white clover and perennial ryegrass herbage were 251·6 and 151·9 g kg⁻¹ DM in Year 1 and 271·9 and 174·0 g kg⁻¹ DM in Year 2 respectively. The CP concentration of the white clover increased significantly during the grazing season from 220·0 to 284·1 g kg⁻¹ DM in Year 1 and from 269·0 to 315·5 g kg⁻¹ DM in Year 2. In the perennial ryegrass herbage the CP concentration increased from 112·2 to 172·6 g kg⁻¹ DM in Year 1 and from 142·7 to 239·5 g kg⁻¹ DM in Year 2. The rate of increase during the season in the CP concentration of the perennial ryegrass herbage was similar to the rate of increase recorded in the white clover herbage.     

香软米滇屯502的选育

 主要介绍了利用云南高杆大穗米质特优的毫皮作母本与省协作组选育的株型紧凑、抗性强的香糯米滇侨20杂交,选育出高产优质抗病香软米新品种——滇屯502,同时系统地介绍了软米、香米、香软米的选育,供育种单位参考.     

ULTRASONOGRAPHIC DETECTION OF FOREIGN BODIES IN SOFT TISSUES USING TURKEY MUSCLE AS A MODEL

Foreign bodies in the soft tissues are frequent problems in both veterinary and human medical practice. Radiography has been the principle tool for diagnosis, but is unable to define many foreign bodies. This study was undertaken to compare the sonographic and radiographic visualization for a variety of foreign bodies. Fresh turkey muscle (pectoral and thigh) with skin was used as the soft tissue model. Assorted foreign bodies were introduced 1 cm into the muscle. Materials examined were small wood fragments, steel wire, glass, nails, graphite, rock, BB pellets, and polystyrene plastic. Following introduction of the foreign bodies radiographs were obtained and the specimens were studied sonographically at 5.0 mHz and 7.5 mHz. All foreign bodies were visible with sonography. A bright echo with either acoustic shadowing or reverberations was present for all specimens. Radiographically metallic foreign bodies were visible but wood, some graphite, and plastic were not seen. We conclude that radiography should remain the primary imaging modality for foreign body detection because of its general availability and low cost. If radiography is negative ultrasound can detect and guide the removal of radiolucent foreign bodies.     

云南软米品质特性比较研究

软米是云南特有的优质米资源,但目前对软米的品质特征仅限于定性的描述。因此,为更有效地利用云南省这一独特的优质米资源,本研究将云南软米与粘米、糯米进行外观品质、碾磨品质、蒸煮食味品质以及营养品质的比较研究。经方差分析表明,3种类型的稻米在粒长、粒宽、长/宽、直链淀粉含量、胶稠度以及蛋白质含量上存在显著差异,尤其在直链淀粉含量上差异极显著(F=119.485**)。指出可以把粒型>3.0,直链淀粉含量7.1%~15.0%,胶稠度45~80 mm,蛋白质含量<9%,并且亲本的系谱中有云南软米地方品种的材料初步鉴定为软米材料。并提出一些加快云南软米资源研究及育种方向的建议,旨在为加快软米育种与生产提供参考。     

海南红肉与白肉火龙果营养成分含量分析

测定海南地区栽培的红肉火龙果和白肉火龙果中营养成分含量,比较两个品种间的差异。结果表明,红肉火龙果的糖酸比、碳水化合物、磷、钠、钙、锌、铜含量高于白肉火龙果,蛋白质、膳食纤维、灰分、脂肪、铁、镁、硒含量低于白肉火龙果;钙含量在两种火龙果中差异显著(P0.05),脂肪、镁含量在两种火龙果中差异极显著(P0.01)。     

境外网络游戏的软权力作用分析

境外网络游戏是软权力在文化领域的重要载体之一,正日益凸显其重要地位,大多是通过网络游戏的内容和背景展现出来,意在增加本国经济效益的同时,以达到能够吸引不同国家和地区的更多玩家,灌输和宣传本国的思想和文化,使之成为配合其各国软权力战略实施的双重作用。面对这种新的文化渗透模式,研究如何应对境外网络游戏的隐性影响,是目前研究的主要课题。     

湖北省潜江地区克氏原螯虾(procambarus clarkii)白斑综合征(White Spot Syndrome)PCR诊断及组织病理学观察

近年来在湖北省范围内人工养殖的克氏原螯虾暴发了严重的疾病,其中白斑综合征病毒（WSSV）已成为危害克氏原螯虾健康养殖的重要病原。2016年5月湖北省潜江市养殖区暴发了一种传染性疾病,为探究此次疾病病因和流行规律,将染病虾进行临床症状观察、对病料进行PCR检测、系统发育树分析、人工感染和组织病理学观察。结果显示,发病克氏原螯虾临床症状主要表现为摄食减少,活力下降,反应迟钝;组织病理学观察结果显示,克氏原螯虾的肝胰腺、肠、肌肉、鳃组织均出现不同程度变性和坏死以及炎性细胞浸润等典型病理学变化,与WSSV感染克氏原螯虾出现的病变相似;PCR检测患病克氏原螯虾样品,结果显示WSSV呈阳性,阳性检出率为55.56%（15/27）,未检测到斑节对虾杆状病毒（MBV）和传染性皮下及造血组织坏死病毒（IHHNV）;检测产物测序并进行系统发育树分析,结果显示,该基因序列与WSSV的EG3株（KR083866.1）核苷酸序列同源性为100%。将病虾的肝胰腺、肠和肌肉组织投喂健康克氏原螯虾,投喂组均表现为急性死亡（累积死亡率为100%）,并出现与自然发病虾相同的症状。WSSV的巢式PCR检测结果显示,人工感染病虾为WSSV阳性。根据以上显示,本次养殖克氏原螯虾大规模死亡的病原是WSSV。     

Subcutaneous Soft Tissue Sarcoma with Rhabdoid Features in a Dog

A nine-year-old male beagle dog had a white spherical mass in the subcutis of the left lumbar region. Microscopically, spindle to oval cells diffusely proliferated in the fibrous and myxoid stroma. Many neoplastic cells showed rhabdoid features or vacuolated cytoplasm. Immunohistochemically, the neoplastic cells were positive for vimentin and S100 and partly positive for neuron-specific enolase and glial fibrillary acidic protein but were negative for von Willebrand factor, desmin and α-smooth muscle actin. Ultrastructurally, the neoplastic cells had abundant cytoplasmic processes and desmosome-like structures. Cytoplasmic inclusions of rhabdoid-featured cells in HE sections were composed of aggregates of intermediate filaments, and cytoplasmic vacuoles were identified as an invagination of cytoplasm. Although malignant peripheral nerve sheath tumor was suggested according to these results, the present case was diagnosed as a soft tissue sarcoma with rhabdoid features due to a lack of identification of the basal lamina under electron microscopy.