苍术和艾叶用于马铃薯组培室熏蒸的研究

通过苍术、艾叶与甲醛的熏蒸效果比较,探索中药在组培室消毒中的可行性。试验结果表明:苍术的平均灭菌率为44.8%(真菌)、77.9%(细菌),艾叶的平均灭菌率为68.4%(真菌)、44.1%(细菌)。甲醛熏蒸平均灭菌率为70.2%(真菌)、68.8%(细菌)。由于苍术对细菌的灭菌效果优于真菌,而艾叶对真菌的灭菌效果优于细菌,可根据组培真、细菌污染的实际情况选择二者之一作为熏蒸剂。两种中药熏蒸剂对人体和试管苗均无伤害,且与甲醛熏蒸效果相近,可用于马铃薯组培室的消毒杀菌。     

土壤熏蒸剂棉隆联合太阳能消毒防治草莓土传病害效果及经济效益分析

太阳能消毒技术在世界范围内广泛使用, 由于其经常受到气候差异的影响导致效果不稳定, 通常与其他措施结合以加强防治效果。种植前采用土壤熏蒸是土传病害的有效预防策略, 本研究通过监测土壤温度、理化性质、土传病原菌、草莓植株长势、产量和分析经济效益, 评价了不同浓度的土壤熏蒸剂棉隆和太阳能消毒联合处理对草莓土传病害的防治效果及经济效益分析。种植前棉隆熏蒸和太阳能消毒处理不仅能很好地控制土传病害, 其对镰刀菌属、疫霉属的抑制率分别为64.41%~84.75%、51.59%~86.94%, 而且显著提高了草莓的产量, 增产率为79.9%~99.4%;联合处理的成本较单独太阳能消毒处理仅增加约3.29%~13.17%, 但净收入增长率高达49.77%~66.28%。因此, 在草莓土传病害管理中, 土壤熏蒸与太阳能消毒处理相结合, 可以降低作物感染土传病害的风险, 保证作物稳定高产。     

仓底混合和仓顶缓释熏蒸筒仓试验

用磷化氢、二氧化碳仓外筒仓底部施药，并结合仓顶缓释投药熏蒸储粮进行试验。结果表明，在未用环流、通风设备等设施的情况下，采用此方法熏蒸高度20m以下的筒仓，可有效地防治储存小麦中的谷蠹RizoperthadominicaFabricius、玉米象SitophiluszeamaisMotschulusky等储粮害虫，具有投资少，操作简便，防治效果好等特点。     

磷化铝间歇熏蒸对锈赤扁谷盗致死效果研究

本文采用磷化铝间歇熏蒸法，使粮堆内部各处PH3浓度在一定的时间内保持在有效浓度之上，无虫期超过12个月，有效地防治了锈赤扁谷盗。     

Urocortin,a novel peptide of the corticotropin releasing hormone family

Urocortin, a novel peptide of the cortiotropin releasiong hormone (CRH) family, was discovered in 1995 in the rat brain and subsequently cloned and localized to human chromosome 2. It can bind to both CRH type 1 receptor and CRH type 2 receptor, as well as CRH binding protein. Especially to CRH type 2 receptor, urocortin binds to it with 40 times more potency than CRH does. Therefore, urocortin is thought to be an en dogenous ligand for CRH type 2 receptor. Immunoreactive urocortin and urocortin mRNA were localized in many areas such as mammalian brain, cardiovascular system, placenta. It is believed that urocortinmay play important physiological roles as a neuropeptide not only in the central nervous system but also in peripheral tissues.     

散装小麦局部环流熏蒸杀虫实仓试验

针对小麦仓中下层局部生虫，采用粮堆局部处理机环流熏蒸，分两次投药，共计熏蒸处理时间为两周。实验证明：在处理部位PH3浓度100～300ppm可以保持12天左右，粮堆中的害虫全部杀死；经检查埋入粮堆的赤拟谷盗、谷蠹和玉米象，成虫死亡率100％，对虫粮培养42天后，检查F1代为零。     

磷化氢环流熏蒸技术在高大房式仓中的应用

在高大房式仓中进行了磷化氢环流熏蒸试验，同时研究了不同仓型和不同熏蒸方式存在的差异。结果表明，在仓房容量、风道设置、环流设置配置合理的条件下，能在较短时间内使磷化氢气体在仓内各部位分布均匀，最低浓度与最高浓度比值最小为0．5，大部分比值在0．8－0．9之间，均高于国外资料推荐的PH3环流熏蒸浓度均匀性标准，即大于0．25。保持有效杀虫浓度的时间长，能够杀死仓内粮堆中的储粮害虫，取得了良好的杀虫效果。     

In vitro conservation of tropical plant germplasm — a review

Summary In vitro medium term conservation of tropical plant germplasm is used routinely in many laboratories. Growth reduction is achieved by modifying various parameters, such as temperature, culture medium, gaseous environment. For long term conservation, cryopreservation (i.e. storage in liquid nitrogen, –196°C) is the only current method available. Each successive step of the process requires precise conditions which have to be defined for each material. Cryopreservation protocols have been set up for more than 40 tropical species. Results obtained with various culture systems such as cell suspensions, protoplasts, calluses, meristems and embryos are discussed. The first example of the large scale application of cryopreservation (oil palm somatic embryos) is presented.     

Genes conferring low seedling reaction to Mexican pathotypes of Puccinia recondita f. sp. tritici,and adult-plant responses of recent wheat cultivars from the former USSR

Fifty-five spring bread wheat (Triticum aestivum L.) cultivars, mostly released between 1975 and 1991 in eight leaf rust-prone spring wheat growing regions of the former USSR, were tested in the seedling growth stage for reaction to 15 Mexican pathotypes of Puccinia recondita f. sp. tritici. In total, seven known and at least two unknown genes were identified, either singly or in combinations: Lr3 (7 cultivars), Lr10 (14), Lr13 (5), Lr14a (1), Lr16 (1), Lr23 (3); the unknown genes were identified in 14 cultivars. The first unknown gene could be either Lr9, Lr19, or Lr25; however, the second unknown gene in 9 cultivars was different from any named gene. Twelve of the 15 pathotypes are virulent for this gene, hence its use in breeding for resistance will be limited. The cultivars were also evaluated at two field locations in Mexico with two pathotypes in separate experiments. The area under the disease progress curve and the final disease rating of the cultivars indicated genetic diversity for genes conferring adult plant resistance. based on the symptoms of the leaf tip necrosis in adult plants, resistance gene Lr34 could be present in at least 20 cultivars. More than half of the cultivars carry high to moderate levels of adult plant resistance and were distributed in each region.     

Inheritance of slow rusting to stem rust in wheat

Summary The inheritance of the slow rusting character was studied on F₅ progenies from seven spring wheat cultivars (Triticum aestivum) crossed in all possible combinations without reciprocals. The cultivars and their progenies were evaluated for slow rusting in 1974 and 1975 in epidemics of Puccinia graminis f. sp. tritici, races 15 and 151, and traces of other races. Slow rusting varied significantly among the parents and among the F₅ progeny of each cross. Transgressive segregation occurred in each cross, i.e. some progeny rusted more slowly than the parents and some faster. In crosses with both Idaed 59 and Kenya 58 the progeny distributions were skewed towards slow rust development but the distributions in the other crosses were normal. The genetic control of slow rusting was predominantly additive, and narrow sense heritability was approximately 80 percent. The number of segregating genes having an effect on slow rusting was estimated to be 2 to 12 pairs depending on the cross. Correlation between slow rusting and maturity was usually negative but in most crosses the relationship was small.Contribution No. 9624 from the Agricultural Experiment Station, University of Minnesota, St. Paul, Minnesota 55108.