茶树小分子量热激蛋白基因CsHSP17.2的克隆与表达分析

[目的]进一步了解茶树小分子量热激蛋白基因CsHSP17.2在逆境胁迫条件下的分子生物学功能.[方法]利用RT-PCR技术从茶树‘迎霜’中克隆得到CsHSP1 7.2基因,运用生物信息学软件分析其核苷酸和编码蛋白,通过Real-timePCR分析其表达模式.[结果]该基因开放阅读框长度为453 bp,编码150个氨基酸,蛋白质相对分子质量为17.2×10a,理论等电点5.56;无信号肽位点,属于非分泌型蛋白;被定位于细胞质中.系统发育树分析表明,茶树CsHSP1 7.2与水稻(GenBank登录号:P27777)和花生(ABC41131)的进化关系较近,属于小分子量热激蛋白基因家族第Ⅰ亚族.qRT-PCR分析发现,茶树CsHSP17.2属于组成型基因;高温(38℃)处理1h能显著提高CsHSP17.2 mRNA的相对表达量(P＜0.05);干旱(100 g·L-1 PEG 6000)、高盐(200 mmol· L-1 NaCl)和外源脱落酸(200 mg· L-1 ABA)处理条件下,该基因的转录水平均出现不同程度的上调.[结论]克隆得到茶树‘迎霜’小分子量热激蛋白基因CsHSP17.2,其在花中表达量最高,且响应高温、干旱、高盐和外源脱落酸胁迫.     

Impact of urban park’s tree,grass and waterbody on microclimate in hot summer days: A case study of Olympic Park in Beijing,China

Urban heat island (UHI) often cause negative impact and has been linked to heart stroke rate, morality, human comfort, energy consumption and air pollution. Fortunately, urban green spaces have been considered as an effective element to mitigate UHI through their cooling effect. However, further empirical research is necessary in order to efficiently guide the design and planning of urban green space. We observed the impacts of urban park's tree, grass and waterbody on microclimate inside the Olympic park of Beijing during summer days. The results indicated that, on average, the park was 0.48–1.12 °C cooler during the day, as well as increased air humidity 2.39–3.74% and reduced human comfort index 1.02–2.43 to generate more comfortable thermal environment. Urban park's cluster trees with short ground vegetation generated higher cooling effect than single trees, grass and waterbodies; proper irrigation regime enhanced the cooling effect of grasses, even the irrigated grass have similar cooling effect with small waterbody on sunny, windless summer days. Therefore, we advise to increase undergrowth coverage and grass irrigation management in order to take advantage of cooling effect of urban parks.     

宁夏枸杞徒长枝青贮研究

枸杞(Lycium bararum)徒长枝是枸杞种植中的废弃物,但含有丰富的营养成分和药用成分,将其开发为青贮中药饲料添加剂是变废为宝的途径之一。本研究以糖蜜、麸皮、玉米(Zea mays)粉为糖源,以亚芯、君安酵宝、益加益、农富康为菌剂,通过比较不同糖源和菌剂青贮的枸杞徒长枝的感官评价得分、营养成分和药用成分等品质特性,探索适合枸杞徒长枝青贮的商品菌剂和糖源。结果表明:3种糖源青贮中酸性洗涤纤维、中性洗涤纤维和氨态氮含量无显著差异(P>0.05),而添加糖蜜的粗蛋白(1.18%)、粗灰分(12.77%)、干物质(30.5%)、乳酸(123.75μg·g^-1)、多糖(27.89 mg·g^-1)、甜菜碱(6.47 mg·g^-1)和黄酮(1.15 mg·g^-1)含量最高,显著高于其余两个处理(P<0.05);4种菌剂和自然青贮的干物质、氨态氮的含量之间无显著差异(P>0.05),亚芯菌剂与自然青贮的粗蛋白和酸性洗涤纤维的含量之间无显著差异(P>0.05);但粗蛋白(1.96%)、酸性洗涤纤维(75.52%)、中性洗涤纤维(73.86%)、水溶性碳水化合物(259.19 mg·g^-1)、乳酸(145.82μg·g^-1)、多糖(27.27 mg·g^-1)含量以自然青贮为最高,且显著高于其余处理(P<0.05)。糖蜜是枸杞徒长枝青贮最佳添加糖源,自然青贮枸杞徒长枝是最佳青贮方式。     

The role of green roofs in mitigating Urban Heat Island effects in the metropolitan area of Adelaide,South Australia

Changing an urban environment and replacing vegetated surfaces with low albedo materials is one of the reasons for increasing temperatures in an urban environment and consequently also one of the key causes of urban heat island effects. In this study, an experimental investigation at the micro-scale and also a numerical simulation at the macro-scale of a typical urban environment in Adelaide were conducted to estimate the potential for mitigating the UHI effect. The results showed that existing low albedo materials such as asphalt, metal roofs and brick pavements contribute to the heat island potential. Also, urban development and a lack of natural vegetation contribute to increased temperatures in cities. The ability of two types of extensive and intensive green roofs to reduce the surrounding micro-climate temperature were monitored. The results showed that they have significant cooling effects in summer time and could behave as an insulation layer to keep buildings warmer in the winter. Furthermore, different scenarios of adding green roofs to the Adelaide urban environment were investigated using the Envi–MET model. The scenario modelling of adding green roofs in a typical urban area in Adelaide, Australia, supported the hypothesis that this can lead to reductions in energy consumption in the Adelaide urban environment. Also an increased use of other water sensitive urban design technologies such as green walls and street trees together with the adoption of high albedo materials is recommended for achieving the optimum efficiency in terms of reducing urban temperatures and mitigating urban heat island effects.     

Acquired Thermo‐Tolerance and Trans‐Generational Heat Stress Response at Flowering in Rice

In rice, pre‐exposure to sublethal treatment followed by harsh lethal treatment is known to improve tolerance of different abiotic stresses at the vegetative stage within and across generations. Our major aim was to test the phenomenon of thermo‐tolerance at flowering across (trans)‐generations and within generation using rice cultivars contrasting for heat stress tolerance at flowering. To test trans‐generational response, plants were exposed to higher temperature at flowering stage and seeds obtained from previous generations were exposed to heat stress during flowering, which recorded significantly lower fertility when exposed to the same degree of stress in their subsequent generations. A pre‐acclimation to moderately high acclimating temperatures imposed over three different durations during the vegetative and initial reproductive stage showed positive response in the tolerant N22, particularly under severe heat stress (40 °C). This finding indicates the possibility of acquiring ameliorative thermo‐tolerant mechanisms at anthesis, restricted to tolerant genetic backgrounds to combat subsequent harsh conditions within the same generation. However, trans‐generational memory was ineffective in mitigating spikelet sterility losses in both tolerant and susceptible backgrounds. Rice is extremely sensitive to heat stress during flowering; hence, similar exercise across other crops of interest needs to be carried out before generalizing conclusions.     

Dry heat treatment of Andean lupin seed to reduce anthracnose infection

The potential of dry heat treatment of Andean lupin seed to reduce seed–borne infection of the anthracnose pathogen, Colletotrichum acutatum, was investigated. First, the effect of dry heat (65 °C) over duration times of 0–96 h on germination and disease incidence after germination was evaluated for artificially– and naturally–infected seed. Dry heat treatment from 8 to 96 h reduced disease incidence after germination to undetectable levels in four cultivars compared with 7.5% disease incidence after germination in seed maintained at room temperature. Moreover, heat treatments of 4–12 h showed seed germination rates that were equivalent to the non–treated control. Under greenhouse conditions, dry heat treatments for 8 or 12 h reduced transmission of the pathogen from seed by 75 or 85%, respectively and dry heat treatment increased emergence of seedlings in comparison the non-treated control. Dry heat treatment is an environmentally friendly alternative for reducing anthracnose infections in Andean lupin seed.     

相变材料在绿色储粮中的应用探讨

相变材料能够储存或释放显热,在熔化或凝固过程中虽然温度不变但吸收的潜热却相当大。根据相变材料可以吸收环境热能并在需要时向环境发出热能的特征,研究相变材料在粮食仓储中的应用,减小外界环境对粮仓温度的影响,达到恒温绿色生态储粮的目的。     

火灾环境下邻近罐壁热辐射分布实验

油罐火灾蔓延的主要因素是油罐热辐射,在着火罐火焰热辐射作用下,邻近罐极易被引燃,造成整个罐区大面积起火。为了研究邻近罐罐壁热辐射分布规律,建立了油罐燃烧对邻近罐热辐射影响的小尺寸实验装置,开展了火灾环境下邻近罐壁热辐射分布的实验研究。结果表明:在火灾环境下,邻近罐正对着火罐方向的罐壁受辐射作用最大,热辐射从罐顶到罐底逐渐降低、从中心向两边呈轴对称降低;随着L/D(L为相邻两个油罐的距离,D为油罐的直径)增大,邻近罐热辐射下降。实验数据为火灾环境下邻近罐的热辐射研究提供了参考,对防止邻近罐被引燃,做好油罐区火灾爆炸事故的预防具有重大意义。