单季稻褐飞虱空间分布格局及其抽样技术

应用聚集度指标法、Iwao 法和Taylor法等对单季稻褐飞虱的空间分布型进行测定检验,结果表明单季稻褐飞虱呈聚集分布,其聚集强度是随着种群密度升高而增加。在此基础上提出了最佳抽样方式、最佳理论抽样数和序贯抽样模型。     

食品中矮壮素与缩节胺的形成及其检测方法研究进展

植物生长调节剂是一种特殊的含有植物激素活性的农药,能够起到改善农作物质量和控制植物生长的作用,近年来植物生长调节剂的毒性及其残留危害吸引了众多研究人员的关注。其中,矮壮素(CHLM)和缩节胺(MEP)作为常用的2种植物生长调节剂,其使用不当和残留危害所引起的食品安全问题并没有引起足够的重视。最近的研究工作将CHLM和MEP确立为大麦麦芽烘烤过程的副产品,而用于研究的大麦属于生态类型,经验证不含任何植物生长调节剂。由此可知,CHLM和MEP是由食品中的天然成分经过热加工处理后形成的化合物。CHLM和MEP作为新的食品加工污染物已成为食品安全问题的研究热点。对CHLM、MEP在食品中的形成进行了综述,并对其检测技术进行总结,以期为CHLM和MEP的研究提供参考。     

云南蓝莓根癌病病原鉴定及其防控药剂的筛选

研究旨在确定云南省蓝莓主产区发生的蓝莓根癌病病原菌种类,并进行田间防治药剂筛选.采集云南省蓝莓主产区蓝莓根癌病样品,通过发病症状、菌落形态观察、致病性测定、Biolog分析、16S rDNA序列分析比较进行系统鉴定.在发病严重的蓝莓基地采用噻霉酮、申嗪霉素等8种药剂进行田间防治试验.从蓝莓根癌病样品中分离、纯化获得的菌...     

模糊数学感官评价法优化紫薯酸奶加工工艺

以新鲜紫薯和全脂奶粉为主要原料,通过正交试验和模糊综合评价法优化凝固型紫薯汁酸奶的生产工艺.结果表明,在紫薯汁添加量为87.5％,嗜热链球菌和保加利亚乳杆菌含量为1％,白砂糖4％,发酵温度42℃,发酵时间6.4 h的条件下制得的凝固型紫薯酸奶感官评分为92.4分,酸度为88.57°T,花青素含量为2.104 mg/g,其质地均匀、口感黏稠、气味兼具牛奶的醇香和紫薯的香甜,色泽鲜亮呈淡紫粉色,感官品质最好.     

综述式和Seminar教学法在食品专业“分子生物学与生物技术”研究生课程中的应用

从教学内容设计、授课形式、考核模式及结合研究生具体研究课题对食品专业硕士研究生“分子生物学与生物技术”综述式和Seminar教学法进行了阐述。这2种教学方法不仅能够帮助学生全面深入地掌握课程的基本理论知识、熟悉分子生物学研究思路和方法、了解相关研究领域的新进展,并且能够增强硕士研究生对科学研究的兴趣,达到促进研究生科研创新能力培养的目的。     

Multi-electrode 3D resistivity imaging of alfalfa root zone

Information on the amount and spatial distribution of plant roots is increasingly needed for understanding and managing crop behaviour. Soil electrical resistivity (ρ) tomography has been proposed as a non-destructive method for root biomass quantification and mapping in trees but evidence is needed on the applicability of the technique at low root density and in herbaceous plants.We produced high-resolution 3D DC soil resistivity tomograms in containers with bare soil (B), and alfalfa (Medicago sativa L.) (A1) on a silt loam soil, and alfalfa on a loam (A2). Root biomass (RMD), root length density (RLD), soil electrical conductivity (EC) and water content (θ) were measured destructively.The pattern of soil resistivity matched the spatial distribution of θ in bare soil and of RMD in rooted soil. Univariate linear relations were found between ρ and θ in bare soil and between ρ, RLD and RMD in rooted soil. Across all data RMD and soil texture (P < 0.01) explained a high proportion of variability in soil resistivity.This allows to conclude that soil resistivity is quantitatively related to root biomass in herbaceous plants even at low root density (biomass < 0.001 Mg m⁻³), providing a basis for the development of resistivity-founded methods for the non-destructive spatial detection of root mass in situ, but the response in ρ is of the same order of magnitude as the effects of grain size and water content. Therefore in field studies reciprocal masking of low-density roots and other soil features is possible, and the effect of variation in other soil properties should be explicitly addressed.     

肉制品出水原因与控制方法的研究

通过对肉制品加工的各种原辅材料及加工的各个环节入手,分析了可能引起肉制品出水的原因,并从原辅材料的选用及加工工艺的改进和优化方面,提出了对肉制品出水问题控制的方法与措施。     

钼酸铵法测定水稻过氧化氢酶活性

钼酸铵法测定植物CAT活性,具有准确、方便、高效的特点.以内两优6号、秀水09的剑叶为材料,通过与紫外吸收法准确度和精确度的比较,来验证该方法的可靠性.结果表明,两种方法结果均值接近,没有显著性差异.紫外吸收法的变异系数分别是0.78%和0.58%,钼酸铵法的变异系数分别是0.35%和0.42%.     

农杆菌敏感小麦基因型的筛选及其转化

利用C58c1农杆菌菌株（其携带的pPTN290质粒载体上含有nptⅡ 基因和GUS基因）为供体材料和小麦幼胚为受体材料,对我国35个春小麦基因型进行了农杆菌敏感性研究和转化研究。组织化学染色结果表明,PM97034、P187、巴140105、扬麦158、新春9号和克丰6号等基因型农杆菌感染后GUS基因瞬时表达率达到了15.0% ~ 30.0%,显著高于模式基因型Bobwhite。其余幼胚分别在含10-25mg/L G418培养基上诱导愈伤组织和分化植株,抗性再生植株经ELISA检测、PCR检测、叶片离体退绿检测、X-Gluc染色检测和Southerm blot检测,最终从新春9号和PM97034的农秆菌转化效率高于Bobwhite. Southerm blot检测结果同时表明,外源基因在多数转基因植株中表现为单拷贝整合。T₁代植株ELISA检测结果和PCR检测结果表明,外源基因在个别株系中的分离符合孟德尔遗传规律,而在多数株系中的分离偏离了孟德尔遗传规律。阳性植株与阴性植株分离比经X²。适合性检测,表明外源基因在多数株系中的遗传符合3：1分离规律。本文首次对不同小麦基因型进行了农秆菌敏感和转化效率研究,筛选到新春9号和PM97034两个对农杆菌比较敏感且转化效率比较高的基因型,可望广泛用于小麦转基因研究。     

Parametric Study and Design of the Ultimate Strength of Tube gusset Plate Connections

The tube gusset plate connections are widely used in electric transmission towers. However, there is little technical information regarding the behavior of tube gusset plate connections. Thus, a analytical parametric study was conducted to investigate the behavior of tube gusset plate connections. The analytical results were compared with the existing experimental results. It was found that the finite element analysis results were in good agreement with the experimental results. In the parametric study, the effects of geometry, chord load and stiffener on the ultimate strength were considered. Based on the analytical results, a design method for predicting the ultimate strength of tube gusset connections was proposed, and its applicability was verified.