Breeding for resistance to rhizomania in sugar-beet (Beta vulgaris L.)

In breeding for resistance to rhizomania, breeding material was routinely examined for resistance to the rhizomania virus over several years. This study was performed in order to investigate the value of virological examination of sugarbeet breeding material in breeding for resistance to the rhizomania virus. Tests were conducted with varieties susceptible or partially resistant to rhizomania, as well as with several new and more-resistant hybrids. A test on young plants showed that all genotypes contained beet necrotic yellow-vein virus (BNYVV), but that the virus content differed widely. Plants of two commercially used partially resistant varieties had, on average, only half the virus content of susceptible varieties in their roots, and plants of eight new hybrids still in the process of registration contained less than one third of that amount. There was a significant negative correlation between the quantity of BNYVV in young plant roots and final yield under disease conditions in the field. Opportunities for further improvement in the level of the resistance to BNYVV in sugarbeet by means of conventional breeding programmes are discussed.     

The inheritance of resistance to beet necrotic yellow vein virus (BNYVV) in B. vulgaris subsp. maritima, accession WB42: Statistical comparisons with Holly-1-4

In this study, the inheritance of resistance to Beet necrotic yellow vein virus (BNYVV) in accessions Holly-1-4and WB42 was investigated. Crosses between both resistant sources and susceptible parents were carried out and F₁F₂ and BC₁ populations were obtained. Virus concentrations in WB42and its F₁ populations were lower than in Holly-1-4. Observed ratios of susceptible and resistant plants in segregating populations of Holly-1-4 as well as WB42 were in agreement with hypothesis of one dominant major gene. Segregation of plants in F₂ populations obtained from crosses betweenHolly-1-4 and WB42 revealed that the resistance genes in Holly-1-4 and WB42 were nonallelic and linked loci. This revised version was published online in July 2006 with corrections to the Cover Date.     

甜菜丛根病的双抗体夹心酶联免疫吸附测定

对甜菜丛根病毒采用双抗体夹心酶联免疫吸附测（Double antibody sandwich assay,DAS—ELISA）法进行了检测,并对测定方法及检测结果进行了分析讨论。     

甜菜抗丛根病育种研究进展

为了研究甜菜抗丛根病育种的抗性基因来源以及作用特点,笔者归纳了甜菜丛根病抗性的来源,包括Alba抗性源、Rizor和Holly抗性源、WB42抗性源、WB41和WB258抗性源以及R36抗性源,并介绍了5种甜菜丛根病的抗性基因特点.另外,对甜菜抗丛根病资源的育成方法进行了总结,包括回交、杂交和轮回选择.通过这些方法将携...     

甜菜抗（耐）从根病生理基础的研究（Ⅳ）——氨基酸与甜菜抗丛根病的关系

通过比较生理学研究表明，丛根病病毒侵染甜菜后引起了体内氨基酸总量及各种氨基酸代谢的改变。以无病地为对照，病地上各品种叶丛和根中总氨基酸量及各氨基酸相对含量较高；抗病品种较感病品种块根和叶丛中均有相对较高的ＡＳＰ、ＳＥＲ、ＧＬＹ、ＶＡＬ、ＬＹＳ、ＰＲＯ，其中ＰＲＯ增加明显，故这些氨基酸特别是ＰＲＯ累积量可以作为病地选育的生理指标。病地较无病地上的各品种具有较强的氨基酸代谢，以及相对较多的氨基酸参与到植株抗病中去。     

Epidemic progress of beet necrotic yellow vein virus: Evidence from an investigation in Japan spanning half a century

Beet necrotic yellow vein virus (BNYVV) is the causal agent of rhizomania, the most serious sugar beet disease worldwide. Since the first finding in Japan in 1969, BNYVV became widespread throughout Hokkaido in a few decades and led to the introduction of Rz1-resistant sugar beet cultivars in the 1990s. Here, we report the historical progress of the BNYVV epidemic in Hokkaido from 1969 to 2019. Previous analysis on samples from 1991 showed that BNYVV isolates were classified into three strains (named O, D, and T) based on the RNA3-encoded p25 gene. The O-type viruses were widely detected in Hokkaido, while the D- and T-type viruses were detected in limited areas. The RNA5, encoding the p26 gene, was initially contained in some D- and O-type isolates but not in any T-type isolates. Interestingly, recent sample analysis revealed that RNA5-containing T-type viruses, seemingly more virulent than the other two strains, were widely detected in Hokkaido. Additionally, a small group of virus isolates harbouring a new p25 gene (named C) was found in limited areas. These results suggest that the T-type viruses, which accompanied RNA5, have been preferentially spread from a limited area to other districts over the last few decades and that this spread might be strongly associated with the recent introduction of Rz1-resistant sugar beet cultivars. BNYVV-positive samples also contained mainly beet soil-borne virus and traces of beet virus Q, both of which are the first to be recorded in Japan.     

ELISA法早期检测甜菜丛根病毒的初步应用

选用甜菜的根和叶片为试验材料,利用ELISA对新疆甜菜品种丛根病病毒（BNYVV）进行检测。结果表明：（1）在试验所检验的5个品种中,X14号,902号,913号,923号为抗病品种,X15号为感病品种,与田间结果相符。（2）从试验中可以看出用叶片样品的检验结果与用根样的一致,但根样的显色反应更为敏感准确。