PCV2衣壳蛋白羧基端基因在T4噬菌体表面的展示及ELISA方法的建立

用PCR扩增猪圆环病毒Ⅱ型广东分离株的衣壳蛋白羧基端基因,将PCR产物连接到pR质粒,转化DH5α细胞,筛选阳性克隆进行PCR鉴定并测序后,转化E2菌,将重组E2菌与缺陷型噬菌体T4-Z1同源重组后,得到重组噬菌体,经SDS-PAGE和Western blotting分析,表明衣壳蛋白片段在噬菌体表面正确展示,表达的融...     

Optimum Soil Water Content for Chickpea Emergence in Heavy‐Textured Soils of North‐West Bangladesh

Sowing of chickpea in the heavy‐textured soils of north‐west Bangladesh with minimum tillage technology aims to increase the timely planting of large areas during a relatively short sowing window before soil water deficit limits germination and emergence. However, the seedbed conditions into which chickpea is sown need to be better quantified, so that limiting factors which affect germination and emergence can be identified. Two of the soil physical characteristics of importance are soil water and aeration. Growth cabinet studies have identified the fastest germination and emergence of chickpea on representative soils for this area at gravimetric water contents of 17–18 %, whilst soil water contents above and below this delayed germination and emergence. Emergence was recorded at soil water potentials between field capacity (?10 kPa) and wilting point (?1500 kPa). Emergence was possible at lower soil water potentials in the finer textured soil, whilst in coarser textured soil, emergence was still possible at higher soil water potentials.     

三岔子林区黄檗野生种群资源状况的调查与分析

以吉林省三岔子林业局不同林龄的林分为对象,调查与分析黄檗野生种群的资源状况,结果表明:单位面积活立木平均株数在幼龄林林分中占12.3%,在中龄林中占3.8%,在成过熟林中占2.6%,3个龄组林分的平均株数比例为6.2%;单位面积活立木平均蓄积在幼龄林林分中占7.3%,在中龄林中占2.4%,在成过熟林中占1.3%,3个龄组林分的平均蓄积比例为3.7%。     

山东省家蚕种质资源研究进展

山东省蚕业研究所保存的家蚕品种资源,从刚建所的30多个增加到现在的110多个,利用这些品种资源已育成了10对实用品种。为更好地利用种质资源,近年来进行了家蚕抗高温、抗氟及淀粉酶活性等方面的研究。     

加强图书馆联合参考咨询系统的建设,促进文献资源的开发利用

本文简述了文献传递服务发展的基本过程,概括了联合参考咨询网服务模式与内容,对现阶段UCDRS存在的问题进行分析与探讨,提出了解决问题的方法及优化措施,积极整合各类图书馆信息资源和服务力量,促进公益性联合参考咨询系统的发展。     

华北地区旅游型村镇住居模式探讨——以保定涞源白石山景区为例

村镇型旅游发展中,旅游者在当地村民家中留宿,村镇的个性化接待和朴素环境让人们赞不绝口,但由于其自发性和无组织性,现在存在设计不得当,经营模式不健全等弊端.研究以河北省涞源县白石山景区为例,分析其存在的问题,旨在为旅游型住居模式的设计与经营提供更为合理的方法与策略.     

Phosphorus availability and farm structural factors: examining scarcity and oversupply in north‐east Germany

Assessing factors influencing phosphorus (P) availability in soils is important in preventing its overexploitation and excessive application in agricultural systems. Despite high historical P applications in the federal state of Brandenburg (Germany), county data on soil test P (STP) reveal considerable disparities in soil available P. In addition, negative soil balances as a result of small mineral P and manure inputs have been observed, raising questions about the factors leading to this situation. Our work focused on identifying possible causes operating at the farm management level by conducting a letter survey in two administrative districts of Brandenburg, the counties Barnim and Uckermark, linking farm management factors (ownership type, farm size, land tenure, animal husbandry with or without grassland and its intensity, presence of a biogas plant and organic production) to farmers’ self‐indicated levels of STP. Small‐ to medium‐sized individual farms tended to have (very) high STP, while large partnership farms and companies/cooperatives were sensitive to factors resulting in low STP. Farms with low shares of land ownership, the presence of grassland, extensive cattle farming and stockless organic farming had lower STP. On the other hand, biogas plants, partly in combination with intensive livestock (cattle) farming, were associated with larger STP. It was concluded that more care should be devoted to the design of agricultural policies and that further (inter‐ and transdisciplinary) research on this topic is needed.     

峨眉山野生茶树资源农艺性状多样性分析

为挖掘峨眉山野生茶树和群体种中的优良资源,利用因子分析、聚类分析和感官审评等方法,对峨眉山40份野生茶树种质资源农艺性状进行遗传多样性分析和资源评价。结果表明,峨眉山茶树种质资源丰富,且多以灌木型为主;18个芽叶形态性状的变异系数为7.70%~60.99%,变异系数最大的性状是芽叶色泽(60.99%)。因子分析表明,18个芽叶形态性状综合为6个公因子,前6个公因子的特征值大于1且累计贡献率达80.74%,主要代表性指标为叶片大小、叶片锯齿和叶面特征等。聚类分析将研究材料分为5个大的类群,显示了复杂的亲缘关系和丰富的遗传多样性。适制性研究表明,初步筛选的11份种质资源制绿茶适制性较好,感官审评总分在92分以上,大体表现为香气鲜香带花香、清香高长带花香或栗香浓郁带花香,滋味鲜爽、鲜甜或鲜浓醇。     

杭州余杭闲林村集体经济发展现状与对策

浙江省自2021年推进共同富裕示范区建设以来,一直将农村经济的高质量发展作为重点发力方向之一。租赁型城中村作为城市快速发展过程中形成的一种特殊经济体,如何摆脱低质量发展、破解转型升级瓶颈是一项重要课题。本文在给出农村分类和国内城中村发展概况基础上,以杭州市余杭区闲林街道的闲林村为案例,解读其发展现状、剖析其面临的困境和难点,并结合其发展阶段和资源禀赋,据此提出拓展产业合作、盘活闲置资金、优化村企治理与考核等推进租赁型城中村集体经济高质量发展的对策建议。     

抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选

条纹叶枯病是水稻(Oryza sativa L.)上最严重的病害之一。STV11是从籼稻Kasalath中鉴定的条纹叶枯病抗性基因,根据Kasalath型条纹叶枯病基因STV11 KAS6个碱基缺失的功能性多态性序列差异,设计基于PCR的功能性分子标记qstv11。利用此标记对299份试验材料[5份抗病品种、5份感病品种、41份辽宁省育种材料、17份日本粳型材料、142份秋光/七山占重组自交系(群体A)、85份笹锦/Habataki//笹锦回交重组自交系(群体B)、群体亲本4份]进行基因型分析并进行抗病性鉴定。结果表明,qstv11能够较好区分抗感基因型,而且在测试材料中与条纹叶枯抗性整体呈现共分离。41份辽宁材料中鉴定出含有STV11-R(抗)材料1份;日本粳型材料该位点均为STV11-S(感);群体A和B中分别筛选出粳稻背景STV11-R型材料24份和6份。