水产养殖专业英语课程的教学改革与实践

水产养殖专业英语是为了适应社会经济发展而为学生开设的一门考查课,其目的是让学生掌握一种获取更多资料和信息的语言工具.然而,我国至今没有一本适合普通高职院校学生的专业英语教材.根据自身教学实践,本文从教学内容选择及教学手段改革两方面进行了细致深入的阐述,希望对我国水产养殖专业英语或其他学科专业英语的课程教学改革有所启发.     

Homeopathic complex increases survival without affecting the performance of Nile tilapia during masculinization

During the masculinization of Nile tilapia, Oreochromis niloticus, the effect of a homeopathic complex (containing Chamomilla, Quina, and sulphur) was evaluated based on survival, performance, gills and liver integrity, and parasitism. Group fed with basal diet + 17 alpha-methyltestosterone (control) showed low survival (40.02%), while the group that additionally received homeopathy and the group that received sucrose (vehicle) showed 73.7% and 87.7% survival, respectively. The growth performance of the group that received homeopathic and the control group was significantly higher than that of the group that received only sucrose. Still, fish that received the homeopathic complex showed higher branchial and hepatic integrity as compared to the other groups. The homeopathic complex contributes to a greater survival of fish in addition to maintaining satisfactory growth. Furthermore, homeopathy contributes to the improvement in gill and liver integrity of the Nile tilapia larvae.     

三种因素对海水生物流化床启动期间营养盐去除及amoA基因表达的影响

在实验室规模下,以旋转式生物流化床(CB-FSB)为研究对象,研究了初始总氨氮(TAN)、水温及滤料膨胀率3种条件下,海水生物流化床生物过滤功能启动期间TAN和亚硝酸盐氮(NO-2-N)去除及amoA基因数量的变化。结果显示:生物流化床生物过滤功能启动所需时间随着水温的升高而缩短,在水温为15℃、20℃和25℃时,启动所需时间分别为27 d、25 d和23 d;初始TAN质量浓度的升高也会缩短生物流化床生物过滤功能启动所需要的时间,在初始TAN质量浓度为1 mg/L、2 mg/L、4 mg/L时,启动所需时间分别为24 d、22 d和21 d;在膨胀率为100%和150%时,启动所需时间无明显差别,分别为21 d和20 d,明显好于膨胀率为50%时启动所需时间27 d;amoA基因的数量变化与TAN去除率的变化有一定的相关性,并随着初始TAN浓度的升高而增多,在4 mg/L时数量最多,达到2.76×10~7copies/g。     

循环水抽屉式立体养殖系统养殖马粪海胆研究

研究利用循环水养殖设备和多层抽屉式养殖箱构建一种新型的立体循环水养殖系统,对马粪海胆进行为期90 d的养殖试验.试验结果表明,养殖用水在循环使用的情况下,水质指标处于良好状态:NH+4-N为0.02～0.15 mg/L,NO-2-N为0.01～0.35 mg/L,pH值为7.7～8.2;马粪海胆养殖密度可达1 444.23 g/屉(约20 L),特定生长率(SGR)为0.33,成活率为89.15%.     

Scientific uncertainty and the assessment of risks posed by non-native freshwater fishes

The introduction of non‐indigenous plants, animals and pathogens is a pressing global environmental challenge. Although not all introduced species become established and the fraction of those that do often have little appreciable effect on their new ecosystems, many others exert significant ecological, evolutionary and economic impacts. Stimulating further debate, Gozlan [Fish and Fisheries (2008) Vol. 9, pp. 106–115] argued that the majority of intentional freshwater fish introductions associated with aquaculture (fish species providing societal benefits) have not been reported as having an ecological impact. We find little to argue with his suggestion that low risk of ecological impact coupled with high market value encourages further introductions. But do we have an adequate understanding of the ecological risks associated with fish introductions to support such decisions? Indeed, resource managers and decision makers require some scientific knowledge to support their management actions; without this information, a precautionary approach is the only sensible course of action. The precautionary approach implies that the lack of scientific certainty is reason enough for postponing intentional introduction of non‐native species to avoid potentially serious or irreversible harm to the environment. Here, we suggest that we actually know very little about ecological impacts associated with fish introductions and that it would be therefore wholly inappropriate to equate a lack of data with a conclusion of ‘no impact’. We discuss four major challenges for enhancing the assessment of risks posed by non‐native freshwater fishes in the face of scientific uncertainty and highlight research opportunities and some alternative approaches for confronting these challenges in the future.     

Fish stress and mortality can be predicted using reflex impairment

Fish stress and mortality can be significant problems in both capture and culture operations. In addition to inexpensive and real-time metrics, ones that are simple to use are also desirable for measuring fish stress and predicting mortality. Current methods to define stress rely on expensive, laboratory-based measurements of changes in fish pathology such as disease, necropsy and histology, in physiology such as plasma cortisol, lactate, glucose and ions and in complex behaviour determined from swimming, feeding and predator evasion. All of these methods are often not rigorously linked to fitness outcomes. An alternative is to observe reflex impairment as a direct sign of stress which can be easily and rapidly measured in free swimming or restrained fish responding to peripheral stimuli such as gravity, light, sound and touch. Reflex impairment is correlated with stress and mortality outcomes, eliminating the need for prolonged holding or monitoring of fish. A few examples of reflexes that may be impaired include orientation, startle responses, fin erection, body flex upon restraint, operculum and mouth clamping or gaping, gag response and vestibular–ocular response. Reflex impairment combines the effects of stressors and their interactions and is not dependent on fish size, motivation states and acclimation which make it a consistent sign of stress across a wide range of stressor types and fish ages. Use of reflex impairment to measure stress and predict mortality would significantly improve monitoring of fish health and welfare in many types of field operations such as commercial and recreational fishing, aquaculture, live transport, stock enhancement and tagging.     

Using Chlorella vulgaris and iron oxide nanoparticles in a designed bioreactor for aquaculture effluents purification

Aquaculture operations produce high volumes of wastewater containing suspended solids and nutrients such as phosphorus and nitrogen. Treatment of water effluent from fish production ponds is essential for sustain environment. So, the present study was conducted to evaluate simultaneous use of microalgae and iron oxide nanoparticles (NPs) to purify aquaculture effluents within a designed bioreactor. For designing experiment, effluent samples were collected from fish farms in Sari, Iran. Iron oxide nanoparticles were prepared from Iranian Nano Pishgaman Company. Chlorella vulgaris was captured from the environment, then purified and cultured in the laboratory. After that, NP and microalgae were transferred to the reactor space. TSS (total suspended solid), TDS (total dissolved solid), BOD (biological oxygen demand), pH (power of hydrogen), EC (electrical conductivity), NO₃ (nitrates), NO₂ (nitrite), NH₄, (ammonium) and PO₄ (phosphates) were measured during the experiment period which NH₄ (93.67 %), NO₃ (92.23 %), NO₂ (89.3 %), and PO₄ (89.25 %) showed the highest reduction percentage, respectively. Also, significant differences among the calculated parameters (except for pH) were observed during the experiment (P < 0.05). Based on the obtained results, it is concluded that the simultaneous use of microalgae and nanoparticles is desirable for purification of aquaculture wastewaters.     

Microsatellite pedigree analysis reveals high variance in reproductive success and reduced genetic diversity in hatchery-spawned northern abalone

The northern (or pinto) abalone, Haliotis kamtschatkana, is a broadcast-spawning marine gastropod that was recently listed as endangered in Canada. To aid in species recovery, a captive-breeding and supplementation program is underway in Barkley Sound, British Columbia. We genotyped first generation progeny for five microsatellite loci and used a pedigree reconstruction program (Pedigree 2.2) to identify their genealogical relationships in the absence of information on parental genotypes. We analyzed progeny from three separate group-spawning events and inferred considerable variation in the number of offspring produced by each parent; in the most severe case a single male sired all the progeny produced during one spawning event. After only one generation of captive-breeding we found a 55–60% reduction in allelic richness and a 17–18% reduction in heterozygosity relative to the diverse wild source population. This study illustrates the difficulty of managing genetic diversity in hatchery populations of a broadcast-spawning species, even when gametes are collected separately from each individual broodstock.     

大型海藻南方浒苔(Ulva meridionalis)对水产养殖废水净化作用的研究

[目的]为了确定南方浒苔（Ulva meridionalis）对水产养殖废水的净化效率、最适藻体密度和最佳处理时长。[方法]实验以模拟水产养殖废水为藻体培养液,设置0.5、1.0、1.5、2.0、2.5 g/L（实验组）和0 g/L（对照组） 共6个藻体密度梯度,培养于bluepard生化培养箱内,在第0, 12, 24,36,48,60,72 h测定藻体培养液中NH4+-N、NO2--N、NO3--N、PO4--P的浓度。[结果]结果显示,南方浒苔对水产养殖废水具有显著的净化效果（P<0.05）,其中净化养殖废水最适藻体密度为2.5 g/L。在南方浒苔最适藻体密度下有效净化水产养殖废水中NH4+-N、NO2--N、NO3--N、PO4--P时最佳处理时长分别为24、60、48、72 h,其去除率分别可达96.02%、51.83%、80.85%、97.67%。同时,南方浒苔对不同形式无机氮的吸收效率会受到水产养殖废水中不同氮源组成的影响,具体表现为先吸收 NH4+-N再吸收NO3--N 最后吸收NO2--N。[结论]研究表明,藻体密度为2.5 g/L的南方浒苔在水产养殖废水中处理72 h对废水中氮磷净化效果最佳。[意义]本研究可为生物净化水产养殖废水提供数据支持。     

营养液膜技术栽培牧草净化循环流水水产养殖废水的试验

为研究植物生态净化技术集成至循环流水水产养殖系统的可行性,构建了4个(4重复)3.0m×0.5m×0.05m(长×宽×高)的植物滤器处理淡水白鲳(Colossoma brachypomum)高密度养殖废水。每个植物滤器生长0.8m2NFT(营养液膜技术)培多年生黑麦草(Lolium perenneL.)。废水每周更换一次,每次25.4L,连续运行40d。7d循环灌溉处理后,NO3--N、TN、TP、COD的去除率分别达96.1%、86.2%、90.5%、88.7%,pH从6.4升高至8.4,而水体积只消耗29.7%。除UIA由于pH升高而超标外,所有水质指标均符合渔业水质标准。牧草日增长仅2.3mm,显著低于用商品营养液培育的牧草生长速度(日增长13.2mm)。鲜草TAN和NO3--N含量分别为107.9和42.5μg.g-1,试验结束时收获鲜草582.9g.m-2,烘干后得到干草102.5g.m-2,干湿比0.176。结果表明,植物滤器能解决生物滤器引起的N、P、COD累积以及pH下降等问题,是一种环境友好的水处理方法。为使牧草良好生长,建议定期将牧草返回培育系统用商品营养液培养一段时间。