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71.
A pedigree analysis of a family of 15 related Chinese Shar Peis was conducted. This pedigree analysis, including affected and nonaffected dams, sires and offspring, was compiled to document and characterize the occurrence, common clinical signs, and age of onset of primary lens luxation while suggesting a possible mode of inheritance in this breed. Of the five offspring from the mating of an affected dam to two unrelated affected males, 100% of offspring were affected with bilateral primary lens luxations. Of the four viable offspring from the mating of the same affected dam to an unrelated, unaffected male, two dogs (50%) were affected. The average age of onset of affected animals (seven) in this first generation was 4.9 years (range 3–6 years). The six dogs in the second generation of the same pedigree line were 2-years-old at examination with none of these animals affected at the time of this study. The most common presenting complaints were a unilateral change in ocular appearance (5 of 7 dogs) and subjective vision impairment (4 of 7 dogs). The most common clinical sign upon ophthalmic examination was iridodonesis (unilateral 4 of 7 dogs; bilateral 3 of 7 dogs) and the presence of an aphakic crescent (3 of 7 dogs). Gonioscopy and tonometry of severely affected eyes revealed a narrow or closed iridocorneal angle and ocular hypertension. This study suggests that primary lens luxation does occur in the Chinese Shar Pei, resembling the clinical condition (age of onset, clinical signs) previously described in the terrier breeds, the Border Collie, and the Tibetan Terrier. Application of the phenotypic findings in this study to a Mendelian genetic model of inheritance suggests that primary lens luxation in the Chinese Shar Pei is inherited as a simple autosomal recessive trait. 相似文献
72.
笔者从石灰土在道路施工中的重要性出发,分析市政道路石灰土基层施工的前期准备工作,并介绍了市政道路石灰土基层施工工艺流程,最后分析了特殊季节道路石灰土基层施工的注意事项。 相似文献
73.
74.
柔嫩艾美球虫(Eimeria tenella)体外细胞培养 总被引:3,自引:0,他引:3
试验比较了玻璃珠层析法,DEAE纤维素层析法,G3漏斗过滤法3种提纯柔嫩艾美球虫(Eimeriatenella)子孢子的方法,柱高8cm,直径200μm的玻璃株柱用pH8.0的Ringer’s缓冲液作洗脱提纯子孢子,回收率为64.1%,子孢子洗脱高峰期集中,且易于无菌操作,柱高5cm的DEAE纤维素柱用pH8.0的甘氨酸缓冲液作洗脱液,提纯子孢子回收率为63.2%,但严格无菌操作困难,G3漏斗法易 相似文献
75.
无芒雀麦人工草地初级生产力动态的研究 总被引:3,自引:0,他引:3
以无芒雀麦人工草地为研究对象,对其生物量的季节及垂动态进行连续4a的研究。为促进无芒雀麦草地在河南省的高产丰收提供基础资料及其理论依据。 相似文献
76.
旨在对西门塔尔牛胰腺间充质干细胞(pancreatic mesenchymal stem cells,PMSCs)进行原代培养并研究其体外分化潜能,为细胞疗法和组织工程学方面提供新的种子细胞。本研究从3月龄的西门塔尔牛胚胎中无菌分离胰腺组织,分别采用胶原酶消化法和组织块贴壁法分离PMSCs,进行原代培养;绘制第3代、第9代、第15代细胞生长曲线并测定群体倍增时间及克隆形成能力,采用免疫荧光检测干细胞表面标志物(CD29、CD44、CD73、CD90、CD34和CD45),RT-PCR检测干细胞细胞表面标志物(CD29、CD44、CD73、CD90、CD106、CD166、CD34和CD45);染色体核型分析检测其基因稳定性,通过向成脂、成骨、成软骨和成肝样细胞诱导分化,检测其多向分化潜能。结果表明,两种方法都可成功分离出PMSCs,细胞贴壁后形态均为长梭形,漩涡状生长,生长趋势呈典型的S形;第9代细胞群体倍增时间显著低于第15代而高于第3代(P<0.01);第9代PMSCs克隆形成率显著低于第3代而显著高于第15代(P<0.05);免疫荧光结果表明,PMSCs特异性表达CD29、CD44、CD73和CD90,RT-PCR结果显示PMSCs特异性表达CD29、CD44、CD73、CD90、CD106和CD166,未表达造血细胞表面标志物CD34和CD45,与国际细胞治疗学会组织干细胞委员会指定的MSCs表面标记物相对应;核型分析表明,PMSCs为正常二倍体(2n=60,XY),染色体基因组未发生变异;特异性染色和RT-PCR结果表明,从西门塔尔牛体内获得的PMSCs可分化为脂肪细胞、骨细胞、软骨细胞和肝样细胞。本试验证实两种方法均可成功建立西门塔尔牛PMSCs体外分离培养体系,PMSCs具有活性好、增殖速度快的特点,与MSCs有相似的生物学特性和多项分化的潜能。可为组织工程学提供新的种子细胞。 相似文献
77.
奶牛乳腺上皮细胞的原代培养及其生物学特性分析 总被引:1,自引:0,他引:1
旨在从奶牛乳腺组织中分离原代乳腺上皮细胞(bovine mammary epithelial cells,BMECs)并传代培养后探究其生物学特性。本研究从屠宰场采集健康泌乳奶牛乳腺并采用改进的酶消化法从乳腺中分离得到原代奶牛乳腺上皮细胞,通过形态学观察、免疫荧光以及染色体核型分析的方法对其进行鉴定。同时,研究第3、第6和第9代乳腺上皮细胞的生长曲线、群体倍增时间和冻存复苏活力,检测不同代次细胞分泌乳蛋白、乳脂、乳糖的功能及泌乳相关基因的表达。结果表明,所分离的奶牛乳腺上皮细胞纯度较好,细胞生长呈现S型,3个代次细胞的群体倍增时间依次为34.87、41.45和65.04 h,冻存复苏活力为88%~93%;在细胞分泌功能方面,诱导培养2 d后均能检测到酪蛋白、甘油三酯和乳糖,且各代次间无显著差异;此外,3个代次的细胞诱导后均能表达乳成分合成相关基因。本研究成功培养了原代奶牛乳腺上皮细胞,并证明直到第9代细胞仍然具有正常的生物学功能,为体外探究乳腺细胞增殖与分化机制提供了良好的试验材料和技术支撑。 相似文献
78.
T. Dittmar 《Mangroves and Salt Marshes》1999,3(3):185-195
During the dry season, dissolved inorganic nutrients and organic carbon (DOC) in a mangrove tidal creek (Bragança, North Brazil) presented a clear tidal signature: silicate, phosphate, ammonium and DOC covaried with salinity, with minima at high tide and maxima at low tide. Hydrodynamical calculations explained most diel variability as dilution of nutrientrich groundwater input by estuarine water. Creek nutrients tended to lower concentrations towards the end of the dry season, probably due to changes in groundwater dynamics. Ammonium was about 44% higher in the night than during the day, while silicate, phosphate nitrate + nitrite showed differences < 5%, indicating preferential autotrophic uptake of ammonium as nitrogen source. Nitrification and/or nitrogen fixation are probably sources of nitrate + nitrite, which contributed only 12% to the dissolved inorganic nitrogen pool. Dissolved oxygen (7%) and DOC (11%) were lower during the night, suggesting the existence of a labile, algalderived DOC pool. There is a loose coupling between aquatic primary production, groundwater dynamics and tidal regime. 相似文献
79.
80.
A study on dry matter production and nutrient cycling in agroforestry systems of mandarin grown in association with N2-fixing Albizia and mixed tree species (non-N2-fixing) was carried out in the Sikkim Himalaya. A site with Albizia was referred asAlbizia-mandarin stand and the other site with mixed tree species as mandarin stand. The stand total biomass, net primary productivity and mandarin fruit production was higher under the influence of Albizia. Agronomic yield of crops remained nearly the same in both the stands. Nitrogen and phosphorus concentrations of different components of Albizia were higher than those of mixed tree species, whereas their back translocation from leaf to branch before abscission was lower inAlbizia. The mandarin-based agroforestry is a highly nutrient-exhaustive system evaluated in terms of nutrient exit through the removal of agronomic yield. This system, under the influence of Albizia, was more productive with faster rates of nutrient cycling. Nutrient use efficiency increased under the influence of Albizia, in contrary to the hypothesis that efficiency should decrease with increasing rate of uptake. The poor nutrient conservation of Albizia, and malleability of nutrient cycling under its influence make it an excellent association which promotes higher availability and faster cycling of nutrients.Albizia should be utilised more extensively in the management of mandarin-based agroforestry systems. 相似文献