天然生胶杂质含量测量结果不确定度评定

根据GB/T 8086—2008规定的测定方法,分析了测定过程中的随机效应与系统效应,计算这两种效应对测定过程导致的不确定度和,最终对本方法所产生的不确定度给予综合评定。     

棉铃发育过程中不同组织IAA含量变化特点

为了解IAA与棉纤维品质形成间的关系及棉铃各部位IAA积累转运特点,本研究分析了棉铃各部分在发育过程中IAA含量的变化特点。通过选择陆地棉标准系TM-1、长纤维种质‘母35’和短纤维种质‘208’3个纤维长度差异较大的陆地棉材料,利用酶联免疫法对浙江杭州、海南三亚两地田间试验的各材料不同发育阶段的棉仁、棉籽壳、棉纤维、棉铃壳进行了IAA含量的测定。结果表明,不同参试材料的棉仁IAA含量存在明显差异,棉籽壳、棉纤维与棉铃壳的IAA含量峰值出现时间晚于棉仁,棉铃各个组织IAA含量具有随棉铃组织从内向外逐渐降低的趋势。研究表明,IAA可能是棉纤维发育的正效应因子,且棉铃发育过程中IAA可能呈初期在棉仁富集、随发育进程沿棉铃组织由内向外运输的规律。     

不同胡椒粉中胡椒碱的含量比较

采用高效液相色谱法(HPLC)和紫外分光光度法(UV)测定黑胡椒、胡椒根、市售胡椒粉和用不同浸泡液浸泡脱皮的白胡椒粉中的胡椒碱含量。结果表明：黑胡椒粉、2种市售胡椒粉和白胡椒粉中胡椒碱含量不同；果＞根、白胡椒＞黑胡椒、盐水浸泡＞河水浸泡＞醋浸泡、西安胡椒粉＞海南胡椒粉。说明不同的胡椒粉胡椒碱的含量不同，这可以为胡椒的质量控制提供比较有用的参考。     

Lipid-rich bovine serum albumin improves the viability and hatching ability of porcine blastocysts produced in vitro

The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium (PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3, CPT1, CPT2 and KAT) in Day-7 blastocysts were significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching ability and quality of porcine blastocysts produced in vitro, as determined by ATP content, blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of LR-BSA arise from lipids bound to albumin.     

Effects of Different Substrate Composition on Growth of Gesneriaceae Plants

The cultivation experiment was carried out to investigate the effects of different proportions of peat soil, perlite, vermiculite and yellow mud on growth of Gesneriaceae species(Chirita gueilinensis, Sinningia speciosa, Lysionotus pauciflorus, Hemiboea henryi, Aeschynanthus acuminatus, Saintpaulia ionantha). The growth traits of each plant growing in 7 different matrix materials were investigated.The plant height, crown width and chlorophyll content of each plant were measured. The results showed that the best substrate ratio was peat soil∶vermiculite=2∶1for C. gueilinensis, L. pauciflorus and H. henryi; peat soil∶perlite∶vermiculite = 2∶1∶1for S. ionantha; peat soil∶vermiculite∶yellow mud=2∶1∶1 for S. speciosa; peat soil∶perlite∶vermiculite∶yellow mud=2∶1∶1∶1 for A. acuminatus.     

A calibration method of detecting soil water content based on the information-sharing in wireless sensor network

In agricultural production, there is contradiction between the cost and accuracy of detection during the course of acquiring soil water content (θ) online. This conflict is one of the core issues of automatic water-saving irrigation technology in agriculture. At the same time, capacitive soil moisture sensor (CSMS) has received considerable attention, for it can acquire θ with low cost and high precision, and meet the application requirements of wireless sensor network (WSN). But CSMS is vulnerable to the soil temperature (T_s) and salinity (S_s) in the measurement process. Therefore, this study took EC-5 sensor for example to establish water detection calibration models of soil temperature and salinity for single sensor, using Least Squares Support Vector Machines on MatLAB (LS-SVMlab) as the tool. On this basis, we explored the spatial variability of T_s and S_s, and then a method, which could be used to calibrate the output signals of sensors in multi-point network, was proposed based on the information-sharing (T_s or S_s) technology of WSN. Through laboratory experiment, we effectively reduced the impact of soil temperature and salinity on the single sensor. In example analysis, we investigated the detection precision and costs under different information-sharing radiuses (r). And the results indicated that the method we proposed based on the information-sharing technology of WSN could successfully calibrate the influence of soil temperature and salinity on sensors in multi-point network, and it was an efficacious approach to determine the balance between the calibration accuracy of moisture sensor and the investment of agricultural production. For example, while the calibration precision of soil temperature and salinity is respectively 1%, the costs can be reduced by 30%.     

Physiological processes associated with salinity tolerance in an alfalfa half‐sib family

We previously reported an alfalfa half‐sib family, HS‐B, with improved salt tolerance, compared to parental plants, P‐B. In this study, we conducted additional experiments to address potential physiological mechanisms that may contribute to salt tolerance in HS‐B. Vegetatively propagated HS‐B and P‐B plants were treated with a nutrient solution (control) or a nutrient solution containing NaCl (EC = 12 dS/m). Shoots and roots were harvested at various time points after treatment for quantification of proline, soluble sugar, and H₂O₂ using spectrophotometers. Subcellular localization and quantification of Na in roots were conducted using a Na⁺‐specific dye under a confocal microscope. HS‐B produced 86 and 89% greater shoot and root dry biomass, respectively, compared to parental plants, P‐B, under salinity in the greenhouse. Under saline conditions the HS‐B shoots accumulated 115% and roots 55% more soluble sugars than P‐B counterparts. The non‐saline HS‐B shoots, however, showed 72% less soluble sugars than the non‐saline P‐B plants. Under saline conditions HS‐B accumulated 39% less proline in shoots, while roots accumulated 56% more proline, compared to their P‐B parents. HS‐B plants also showed a greater reduction of stomatal conductance under mild saline stress. HS‐B shoots and roots contained 3–4 times less reactive oxygen species (H₂O₂) after salt treatment compared to P‐B plants. Sodium localization and distribution analysis using Na⁺‐specific dye revealed HS‐B plants accumulated 88% and 48% less Na⁺ in stele and xylem vessels compared to P‐B. The study provides insights into the potential mechanisms that may contribute to salt tolerance in HS‐B: maintaining RWC by accumulating soluble sugars while reducing transpiration, maintaining healthy status by reducing oxidative stresses, and preventing salt toxicity by reducing accumulation of Na⁺ inside root cells and xylem.     

新疆甜菜丸粒化单粒种高密高产高糖栽培模式

在新疆适宜种植甜菜的生态区,通过选择甜菜丸粒化单粒种,在高密度种植条件下,依据不同栽培管理阶段特点采用合理的管理措施,以实现甜菜单产97.5～105.0t/hm2、含糖率15%以上的目标,从而构建甜菜丸粒化单粒种高密高产高糖栽培模式。     

松茸多糖对缓解运动员有氧运动疲劳恢复应用研究

通过分析松茸多糖对有氧运动所产生的一系列良好效果,进行松茸多糖对运动员疲劳恢复的相关研究,松茸多糖主要涉及到运动员运动过程当中,其机体所产生的血清尿素(BU)含量、血清丙二醛(MDA)含量、血清肌酸激酶(CK)、血清超氧化物歧化酶(SOD)以及谷胱甘肽过氧化物酶(GSH-PX)活性的变化。相关研究结果表明,松茸多糖能够对此起到良好的修复作用,有效抑制运动过后所产生的骨骼肌损伤,增强运动员机体抗氧化能力,从而达到恢复疲劳的作用。     

安络小皮伞提取物对运动外伤的镇痛作用

为研究安络小皮伞提取物对运动外伤的镇痛作用,对其所含生物碱、多糖和麦角甾醇类等物质进行了定性检测并进行了小鼠醋酸扭体和小鼠热板镇痛试验。结果表明,安络小皮伞提取物中含有的成分能够消除并缓解小鼠各种神经疼痛,具有抗炎镇痛的功效。