用分子生物学方法鉴别检测动物源性饲料中的牛羊源性成分

为了防止海绵状脑病疫区和痒病疫区反刍动物源性饲料进入我国 ,非常有必要对贸易往来中的肉骨粉品种来源进行鉴定检测。我们采用分子生物学方法从动物源性饲料中鉴别检测牛、羊特异性线粒体DNA的片段 ,并用限制性内切酶SspⅠ对PCR产物进行酶切鉴定及DNA序列测定。该方法的灵敏度可达 0 .12 5 % ,具有灵敏度高、快速和特异性强的优点。     

Changes in the glutathione thiol-disulfide status in wheat grain by foliar sulphur fertilization: consequences for the rheological properties of dough

Storage proteins and glutathione in wheat play an important role in gluten network formation and can be modified by supplementation of nitrogen (N) and sulphur (S) in wheat plants. The glutathione thiol-disulfide status and its relationship to the molecular weight distribution wheat polymeric protein and dough rheological properties have been examined after different foliar S fertilizations (S derived from micronized elemental S and NS, a mixture of N urea and elemental S) applied at the post-anthesis stage. Changes in levels of reduced glutathione (GSH), glutathione disulfide (GSSG), polymeric protein-glutathione mixed disulfide (PPSSG) were analysed by reversed phase high performance liquid chromatography, during grain development using the wheat cultivars, Soissons and Trémie. During the grain desiccation phase, S supplementation (i) increased the GSSG/GSH ratio by 23–25% (ii) induced PPSSG accumulation, and (iii) decreased the formation of SDS-unextractable polymeric protein (UPP) and its molecular mass distribution. However, simultaneous N and S supplementation results in: (i) a decrease in PPSSG formation by 20–30% and (ii) an increase of UPP by 7–18% by enhancing both the branching of the aggregated proteins and their molecular weight. The mixograph parameters show that all forms of endogenous glutathione are linked to dough weakening and are negatively correlated with dough mixing tolerance, dough strength and consistency, while UPP is positively correlated with dough strength and consistency. These findings indicate that S nutrition influences dynamics of the glutathione forms in the grain and results in modification the degree of polymerization of storage protein. Thus both the changes in the form of glutathione and protein polymerization influence the rheological properties of dough.     

杨、桦林抚育改造技术研究

通过对黑龙江省东部山区10个林业局、427块标准地、1712株解析木的调查研究，得到了杨、桦林的树高、胸径和材积生长曲线，并编制了杨、桦林的抚育间伐控制图。     

直接利用传递函数对分叉结构进行模态综合

建立了一种直接利用传递函数对分叉结构进行模态综合的方法。传统的方法一般都需要对大矩阵进行求逆运算,而此方法只涉及分叉结构结合部的传递函数矩阵的求逆,因此求逆计算量很小,特别是当直接采用传递函数的测量值进行模态综合时,就更体现出它的优越性。     

用简易诊断法检查判断液压系统各元件的工作性能

故障的排除首先在于判断，目前基层维修网点大多数没有检测仪器和设备，出了故障乱拆乱卸，导致液压元件工作失灵，甚至整车液压系统瘫痪。拖拉机液压系统产生故障的主要原因是液压元件工作性能恶化引起的。为此，介绍了在没有检测仪器和设备的条件下，如何应用简易诊断法检查判断各液压元件的工作性能。     

宿根花卉的特点及其在园林中的配置

根据宿根花卉种类繁多、色彩丰富、生长稳定、抗逆性强的特点,其在园林中的配置,宜于建花坛、花境,也可群植,与背景树相结合,还可作地被植物代替草坪,也可建单类花卉园.     

南、北盘江流域（贵州部分）土地退化类型及机理

在对珠江上游南、北盘江流域（贵州部分）调查的基础上，分析了该流域土地退化成因，原生生态系统的脆弱性只是导致土地退化的一个方面。而人为活动才是土地退化的主要因素。根据土地退化的成因、结果，将珠江上游南、北盘江流域（贵州部分）土地退化划分为：土壤侵蚀、石漠化、土地贫瘠、土壤污染、土壤破坏五种类型，并提出了退化土地的管理及恢复措施。     

Grouped, stacked rods and tapeta lucida in the retina of Japanese anchovy Engraulis japonicus

ABSTRACT: Morphology of the photoreceptor cells and tapetum of the Japanese anchovy Engraulis japonicus was studied by histologically and by chemical analysis. The Japanese anchovy has a duplex retina. The cones form parallel rows consisting of alternately placed long cones and bifid cones. Both types of cones are intimately associated and form triple units that are regularly spaced along the row of cones. The rods are grouped and stacked. This fish has a retinal tapetum lucidum composed of guanine and hypoxanthine. Three structures of the tapetum lucidum were recognized: platelet, diamond and rod types. Photomechanical changes include movements of the photoreceptor cells and the retinal tapetum. The retina of the Japanese anchovy is thought to be highly sensitive and well adapted to a dim light environment.     

我国农村经济组织体系分析

通过分析我国现阶段农村经济组织的组织模式、功能及组织连接机制,指出我国农村经济组织已形成以农户家庭经营为基础层次,以社区合作经济组织、专业合作经济组织及以“公司 农户”为典型的契约型组织分别为第二、三、四层次,效能逐级递增,各层次之间具有互动作用的组织体系。     

论江苏省农机社会化服务体系建设

农机社会化服务体系建设是经济社会发展的产物，是稳定和完善承包责任制，促进农村社会生产力的发展，率先基本实现农业现代化和小康社会的重要措施。实现农机资源的有效配置．科学合理构建农机社会化服务体系框架，以县、乡农机综合服务站为导向，以农机户为主体，以中介服务组织为纽带，是建设具有江苏省特色的农机社会化服务体系的正确途径。