Effects of swimming on experimental endometriosis in rats

AIM To evaluate the effect of swimming on experimental endometriosis in rats. METHODS 80 female SD rats were divided into 8 groups, including control group, model group and animals performed light exercise （swimming once a week）, moderate exercise （swimming 3 times a week）, and intense exercise （swimming 5 times a week） before or after endometriosis induction,10 rats in each group. The mRNA and protein expressions of fatty acid synthase （FAS）, matrix metalloproteinase 9 （MMP9） and proliferating cell nuclear antigen （PCNA） in endometrium of rats were detected. RESULTS The swimming before the induction of the edometriosis lesions did not prove to have aprophylactic role against endometriosis, whereas the swimming after induction of the lesions had a beneficial effect regardless of frequency, with a greater reduction in the groups practicing moderate and intense activity （P<0.05）, an increase in FAS levels and a decrease in MMP9 and PCNA levels were also observed （P<0.05）. CONCLUSION Swimming after induction of the edometriosis is beneficial for the treatment of endometriosis, the mechanism may be related to the expression of FAS, MMP9 and PCNA protein.     

粗腿羊肚菌子囊孢子萌发过程及其细胞核行为

用4μg/mL 4′,6-二脒基-2-苯基吲哚(DAPI)对细胞核染色,采用激光共聚焦显微成像技术观察粗腿羊肚菌(Morchella crassipes)子囊孢子的萌发过程。新鲜的粗腿羊肚菌子囊孢子呈椭圆状,大小13.0~15.3μm×21.5~24.0μm,成熟子囊孢子的细胞核数量为18~32个;CYM液体培养基,23℃培养6h后子囊孢子体积逐渐膨大至初始体积的1.7~2.9倍,泡囊化现象明显;6~12h开始形成芽管,泡囊逐渐消失,出芽点在椭圆形细胞的一端形成或两端先后形成,数量不等的细胞核由子囊孢子内迁至芽管中,形成多核丝状;芽管生长至20~30μm后,在出芽点与孢子连接处形成隔膜,12~24h后,随着芽管的持续生长,形成新的隔膜和菌丝细胞,菌丝细胞多核;芽管及初生菌丝直径6~8μm,约为子囊孢子直径的1/3;24h内没有观察到初生菌丝之间发生细胞融合现象。     

磁化处理的全尾砂料浆沉降参数优化模型

为了提高全尾砂料浆的脱水浓缩效果,将磁化处理技术引入到全尾砂料浆脱水浓缩中,并建立GA-SVM模型优选全尾砂料浆的沉降参数。建立支持向量机（SVM）沉降参数优化模型,以磁感应强度、磁化处理时间、料浆流速、料浆浓度、絮凝剂单耗为输入因子,沉降速度为综合输出因子,通过正交试验建立样本数据对SVM模型进行训练与检验,采用遗传算法（GA）对SVM模型参数进行优化,进而得到磁化全尾砂料浆沉降参数的GA-SVM优化模型。将GA-SVM模型运用到某铁矿磁化全尾砂料浆沉降参数优化中,得到的最佳沉降参数为磁感应强度0.192 T、磁化处理时间1.85 min、料浆速度1.92 m/s、PAC单耗28 g/t,沉降速度可达约155 cm/h。研究表明：适宜的磁化处理条件可提高全尾砂料浆的脱水浓缩效果,节约30.0%~42.5% PAC用量,GA-SVM模型对全尾砂料浆沉降参数预测结果相对误差在5%以内、预测精度高,为全尾砂料浆脱水浓缩及其参数优选提供了一种新思路。     

Effect of CHOP expression knock-down on apoptosis of renal tubular epithelial cells

AIM:To study the effect of C/EBP homologous protein (CHOP) on the apoptosis of renal tubular epithelial HK2 cells. METHODS:The serum mRNA levels of CHOP in the patients with acute kidney injury and healthy controls were detected by qPCR. In vitro, renal tubular epithelial HK2 cells were divided into control group, negative group (transfected with negative control siRNA), si-CHOP group (transfected with CHOP siRNA), and induced by transforming growth factor-β1 (TGF-β1). The viability of the cells was measured by MTT assay, and the apoptotic rate was analyzed by flow cytometry. The protein levels of nuclear antigen Ki-67, proliferating cell nuclear antigen (PCNA), caspase-3 and cleaved caspase-3 were determined by Western blot. RESULTS:Compared with the healthy controls, the serum mRNA levels of CHOP in the patients with acute kidney injury were increased significantly (P<0.05). Transfection with CHOP siRNA significantly decreased the expression of CHOP in the renal tubular epithelial HK2 cells (P<0.05). Knock-down of CHOP expression by siRNA significantly increased the viability of renal tubular epithelial HK2 cells (P<0.05), decreased the apoptotic rate (P<0.05), increased the expression of Ki-67 and PCNA (P<0.05), and down-regulated the protein level of cleaved caspase-3 (P<0.05). CONCLUSION:The serum mRNA levels of CHOP were increased in the patients with acute kidney injury. Knock-down of CHOP expression inhibits the apoptosis of renal tubular epithelial cells by regulating the expression of proliferation-and apoptosis-related proteins.     

隔板长度对紧凑型高速磁力泵水力特性及叶轮径向力的影响

为探究蜗壳内隔板长度对紧凑型高速磁力泵外特性与叶轮径向力的影响,根据蜗壳型式及隔板长度的不同提出6种蜗壳方案.设单蜗壳为方案一,其余双蜗壳方案根据隔板长度从小到大依次设为方案二至方案六.采用ANSYS-CFX软件对不同工况下(0.8Q_d,1.0Q_d,1.2Q_d)各蜗壳方案泵内流场进行数值模拟,得到不同蜗壳方案的泵中心面静压分布云图,并进行径向力分析.采用方案四蜗壳作为泵实型样机进行试验,将试验值与计算结果进行对比.研究结果表明:相较于无隔板的单蜗壳泵,采用有隔板的双蜗壳泵有利于平衡叶轮径向力,在额定流量下单蜗壳在x,y方向的径向力最大分量分别为151.2,149.7 N,是双蜗壳方案四的1.5倍;随着隔板长度的增大,泵的扬程与效率均逐渐提高,叶轮径向力不断减小,3种工况下扬程的模拟值与试验值偏差均小于3.0%;试验表明数值计算结果具有可信性,研究结果可为紧凑型高速磁力泵在提高水力性能以及平衡叶轮径向力方面提供一定参考.     

低模数水玻璃对橡胶轻骨料混凝土力学性能的影响

为了了解不同掺量水玻璃对橡胶轻骨料混凝土(RLC)力学性能的影响,在RLC中加入水玻璃,并用20％粉煤灰代替水泥,制作水玻璃掺量分别为0,2％,4％,6％,8％的混凝土试块.通过核磁共振试验研究其微观孔隙变化及强度形成机理;进行对数函数曲线预测和BP神经网络强度预测,判断其可靠性并对比优劣.结果表明:水玻璃的掺入使RL...     

供体细胞对哺乳动物体细胞核移植的影响

哺乳动物体细胞核移植技术(克隆)在农业、生物技术、医药生产和濒危动物保护等方面具有很大的潜力和应用价值,已成为目前发育生物学研究的重要方法.文章依据近年来国内外在动物克隆技术方面的资料,分析了供体细胞类型、供体细胞动物年龄、供体细胞所处细胞周期、体外传代次数以及供体动物性别等因素对克隆动物效率的影响及可能原因.     

基于ANSYS的间隙式永磁吸附爬壁机器人的磁场分析

针对永磁吸附爬壁机器人,提出了一种新型的采用了矩形永磁体的间隙式吸附方式,并在Ansys的Magnetic-nodal环境下进行了永磁体的磁场分析,得出不同间隙条件下的磁场变化规律和吸附力。永磁体的磁场强度采用数值计算得出解析表达式的方法通常比较复杂。而有限元分析的方法则为磁场分析提供了一条快速、有效的路径。     

基于非下采样Shearlet变换的磁瓦表面裂纹检测

针对磁瓦表面裂纹缺陷图像背景不均匀、对比度低和存在纹理干扰等特点,提出了一种基于非下采样Shearlet变换(Nonsubsampled Shearlet transform,NSST)的裂纹检测方法。首先对原始图像进行多尺度、多方向NSST分解,得到一个低频子带和多个高频子带,然后利用各向异性扩散和改进的γ增强方法对高频子带进行滤波和增强;同时利用二维高斯函数对低频子带进行卷积操作来构造高斯多尺度空间,估计出图像的主要背景,并通过背景差法得到均匀的低频目标图像。最后通过重构NSST系数得到去噪和增强后的均匀目标图像,利用自适应阈值分割和区域连通法提取裂纹缺陷。实验结果表明,所提方法检测准确率达92.5%,优于基于形态学滤波方法、基于Curvelet变换方法和基于Shearlet变换方法等现有磁瓦表面裂纹检测方法。     

永磁变刚度机构柔性机器人力学特性研究

提出了一种柔性机器人用永磁变刚度机构。由于电动机转矩有限,在不用增加绳索拉力的情况下,通过该机构实现了柔性机器人关节更强的变刚度能力。该永磁变刚度机构主要由磁弹簧单元和滑轮绳索单元构成。通过虚位移法建立了弹簧磁力和绳索拉力解析模型,通过实验对永磁体间磁力和绳索上的拉力进行测量,测量结果和模型计算结果基本吻合。其结果表明,永磁体间磁力、绳索拉力和刚度随永磁体间气隙减小呈非线性增加,随永磁体长度和平均半径的增加而增加,保持三角形结构高不变,绳索拉力和刚度随着三角形结构底长的增加而增加,减小滑轮半径,可以进一步增加绳索刚度变化范围。