Comparison of nuclear morphometric parameters in cytologic smears and histologic sections of spontaneous canine tumors

Background — Nuclear morphometry may provide useful diagnostic and prognostic information for neoplasms in animals. Most available data have been obtained from histologic sections. Nuclear morphometry of cytologic smears may provide important pre-operative information.
Objectives — The goal of this study was to compare nuclear morphometric parameters in cytologic smears and histologic sections from spontaneous canine tumors.
Methods — Mean nuclear area (MNA), mean nuclear perimeter (MNP), mean nuclear form factor (FF; nuclear perimeter²/4π nuclear area) and their respective SDs were assessed by image analysis of both hematoxylin and eosin-stained histologic sections and May-Grünwald-Giemsa-stained cytologic smears from the same case in 20 spontaneous canine tumors of different histogenesis. The above parameters were selected as being the best morphometric tools for measuring variation in shape and size in cells after neoplastic transformation. Data were compared by ANOVA with P<.01 considered significant.
Results — There was a significant difference between histologic and cytologic specimens for MNA, MNP, and their SDs. Only the differences between FF and the SD of FF were not statistically significant.
Conclusions — Only nuclear morphometric data related to nuclear shape and nuclear shape variability are comparable between histologic and cytologic specimens. Nuclear area and perimeter may be affected by the different fixation and smear preparation techniques used in histology and cytology.     

Identification of a periorbital wooden foreign body as the cause of chronic ocular discharge in a horse

The clinical, diagnostic and therapeutic features of a horse with a wooden foreign body embedded in the deep portion of the right masseter muscle adjacent to the right orbit are presented. The purpose of this report is to describe the clinical presentation, magnetic resonance imaging findings and treatment of a penetrating wooden foreign body in a horse that had no history of trauma or evidence of a puncture wound. This report documents the usefulness of magnetic resonance imaging to detect a wooden foreign body embedded in the soft tissues of a horse with a chronic copious ocular discharge. Two surgical procedures were necessary, which is a frequent complication encountered with wooden foreign bodies.     

供体细胞对哺乳动物体细胞核移植的影响

哺乳动物体细胞核移植技术(克隆)在农业、生物技术、医药生产和濒危动物保护等方面具有很大的潜力和应用价值,已成为目前发育生物学研究的重要方法.文章依据近年来国内外在动物克隆技术方面的资料,分析了供体细胞类型、供体细胞动物年龄、供体细胞所处细胞周期、体外传代次数以及供体动物性别等因素对克隆动物效率的影响及可能原因.     

表面等离子共振生物传感器检测培氟沙星的方法研究

研究表面等离子共振生物传感器检测缓冲液中培氟沙星的方法,为样品的检测提供依据。采用抑制法,将培氟沙星与卵清蛋白的偶联物固定在芯片上,将抗体与不同浓度的培氟沙星混合后通入芯片表面,优化反应条件,构建标准曲线。结果显示,选择1∶1000稀释的抗体,流速7 μL/min,反应时间4 min,构建标准曲线,可以达到较低的检测限。利用SPR生物传感器建立快速检测缓冲液中培氟沙星的方法,检测限为0.8 ng/mL,检测时间为4 min,为样品检测奠定基础。     

BRAIN MAGNETIC RESONANCE IMAGING CHARACTERISTICS IN DOGS AND CATS WITH CONGENITAL PORTOSYSTEMIC SHUNTS

Animals with a portosystemic shunt (PSS) often have neurologic abnormalities. Diagnostic imaging, including brain magnetic resonance (MR) imaging, is not performed routinely in these animals. In this study, brain MR images were obtained in 13 dogs and three cats with a PSS, and in 15 dogs and five cats that were neurologically normal and used as controls. All animals with a PSS had widened sulci. In addition, 10 out of 13 dogs with a PSS and one out of three cats with a PSS had hyperintense focal areas in the lentiform nuclei on T1-weighted (T1W) images, which did not enhance after intravenous gadolinium. Following surgical correction of the PSS, MR imaging examinations were repeated in one dog and one cat. The hyperintensity of the lentiform nuclei had decreased. This study indicates that MR imaging findings of widened sulci and hyperintensity of the lentiform nuclei on T1W images may be found in dogs and cats with a PSS.     

主动磁悬浮轴承集中控制器的研究

将传统PID理论和LQR理论相结合,提出了一种易于工程实现的交叉PID集中控制器,解决了磁悬浮轴承系统中集中控制器普遍存在的设计复杂、实时计算量大、过度依赖精确的数学模型等缺陷。针对实验室的磁悬浮轴承试验平台,采用Matlab仿真软件进行了交叉PID集中控制系统的仿真研究。仿真结果表明,交叉PID集中控制器具有较好的控制性能,可以实现电磁轴承转子的稳定悬浮。     

磁流体密封液下泵结构设计与试验

为了解决输送核原料的液下泵工作过程中存在无色剧毒氟化氢气体溢出的问题,在此类液下泵的主轴上选取磁流体为密封材料,应用磁流体密封技术,设计了一套采用螺旋齿的磁流体密封结构,并推导出了密封压差设计公式．通过理论分析和试验研究,讨论了液下泵在液体工作环境下的转速、温度、磁流体体积、密封间隙和磁场强度对磁流体密封承压能力的影响．结果表明,设置在液下泵上的磁流体密封结构的承压能力随着转速、温度和密封间隙的增大而下降;随着磁场强度的升高而增大,且密封承压能力试验值最大可达8×10^5Pa;随着磁流体体积的增大而增大,但是当磁流体体积超过一定的临界值后,磁流体密封的承压能力将不再随着磁流体体积的改变而改变,仅仅保持在某一恒定值．该磁流体密封结构的密封方法将会有效地解决输送核原料的液下泵中害氟化氢气体泄漏的问题．     

基于永磁操动机构的真空开关动力学特性分析

真空开关利用新型的永磁机构作为操动系统,具有较高的操作精度与可靠性。在建立了永磁操动机构动态特性数学模型的基础上,分析了永磁机构的运动特性。在试验过程中实测了真空开关的动态参数,将实测结果与理论计算进行分析比较,结果表明数学模型与计算方法准确可靠。     

Inconel 718合金的磁性复合流体抛光工艺

针对Inconel 718合金材料加工中不断提高的表面质量需求,研究了采用磁性复合流体(Magneticcompound fluid)抛光Inconel718合金材料的加工工艺.根据磁性复合流体抛光原理搭建Inconel718合金抛光实验平台,进行不同抛光方向、抛光时间、抛光转速等工艺参数下的Inconel718合金抛...