全文获取类型
收费全文 | 7551篇 |
免费 | 2806篇 |
国内免费 | 312篇 |
专业分类
林业 | 263篇 |
农学 | 647篇 |
基础科学 | 222篇 |
1843篇 | |
综合类 | 548篇 |
农作物 | 247篇 |
水产渔业 | 2079篇 |
畜牧兽医 | 2963篇 |
园艺 | 17篇 |
植物保护 | 1840篇 |
出版年
2024年 | 15篇 |
2023年 | 38篇 |
2022年 | 63篇 |
2021年 | 191篇 |
2020年 | 524篇 |
2019年 | 979篇 |
2018年 | 855篇 |
2017年 | 919篇 |
2016年 | 741篇 |
2015年 | 697篇 |
2014年 | 677篇 |
2013年 | 923篇 |
2012年 | 1086篇 |
2011年 | 664篇 |
2010年 | 582篇 |
2009年 | 295篇 |
2008年 | 292篇 |
2007年 | 175篇 |
2006年 | 155篇 |
2005年 | 140篇 |
2004年 | 128篇 |
2003年 | 129篇 |
2002年 | 99篇 |
2001年 | 90篇 |
2000年 | 103篇 |
1999年 | 24篇 |
1998年 | 11篇 |
1997年 | 8篇 |
1996年 | 13篇 |
1995年 | 2篇 |
1994年 | 9篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 7篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
121.
122.
BACKGROUND: Greenhouse and nursery producers use entomopathogens (nematodes and fungi) to control soil pests. Although it is known that the physical and chemical properties of mineral soil significantly impact upon soil pathogens, the influence of soilless media used for plant production on entomopathogen performance is poorly understood. RESULTS: Survival and foraging distance were differently affected by sand:peat, bark and sawdust media for entomopathogenic nematodes, but not for the immobile fungus Metarhizium anisopliae. Redwood sawdust medium consistently had a negative impact upon entomopathogenic nematodes. Dividing media into individual components supported the hypothesis that redwood sawdust reduced foraging and infection abilities of S. riobrave and H. bacteriophora. Physically altering the components by adding sand significantly improved foraging and infection success for S. riobrave in media not optimum for foraging. CONCLUSION: This study is the first to highlight the importance of selecting the appropriate soilless media and pathogen species combinations to increase efficacy of biological control. H. bacteriophora was able to find hosts in a wider diversity of medium components than S. riobrave, although both nematode species performed well in peat moss and recycled plant material. These results suggest that peat moss, recycled plant material and hardwood bark are components amenable to EPN biological control programs. Copyright © 2012 Society of Chemical Industry 相似文献
123.
Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia 下载免费PDF全文
E. Abdellatif M. Kałużna J. D. Janse P. Sobiczewski F. Helali J. R. Lamichhane A. Rhouma 《Plant pathology》2017,66(7):1081-1093
In the spring of 2012, symptoms of a disease resembling citrus blast and citrus black pit were observed in some orchards in Tunisia. The epidemic spread rapidly in the following years. Twenty‐four commercial citrus orchards from four Tunisian regions showing characteristic symptoms of bacterial diseases were surveyed during a 3‐year study. Eighty‐eight Pseudomonas‐like bacterial isolates were successfully obtained from the northeast and west of Tunisia. No isolates were recovered from the central region. Overall, 46 isolates were identified as Pseudomonas syringae pv. syringae and most of them showed similar phenotypic and genetic profiles. The virulence of three selected isolates differed from one plant cultivar to another as well as from the type of plant organ used for the inoculation. In a bioassay test, all isolates produced syringomycin, which was confirmed by molecular detection based on the syrB and syrD genes. Only EC122 possessed syrD but not syrB. DNA fingerprints, based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) and PCR melting profile (PCR MP), were used to determine the potential genetic diversity among strains. Clustering of PCR MP fingerprinting data matched with rep‐PCR fingerprinting data. The generated distribution tree showed that Tunisian isolates were closely related to the citrus reference strain LMG5496. In contrast, EC112, isolated from citrus, and the almond isolate EC122 were distantly related to the type strain LMG1247T isolated from lilac. Such studies have not been reported until now for P. syringae from citrus. 相似文献
124.
125.
Romi Novriadi Elizabeth Spangler D. Allen Davis 《Journal of the World Aquaculture Society》2019,50(2):433-447
The present study was designed to investigate the effects of diets containing advanced soy products (enzyme‐treated soy and fermented soy) or corn protein concentrate (CPC) in combination with porcine meal (PM) to completely replace poultry byproduct meal (PBM) on growth performance, body composition, and distal intestine histology of Florida pompano, Trachinotus carolinus. Four experimental diets were formulated to be isonitrogenous and isolipidic, to contain 400 g/kg crude protein and 80 g/kg lipid. A reference diet (PBM diet [PBMD]) contained 150 g/kg PBM and 495 g/kg soybean meal (SBM), and three test diets were formulated replacing PBM with 15 g/kg of CPC (CPC diet [CPCD]) or replacing all SBM and PBM with 535 g/kg fermented soy (fermented soybean meal diet [FSBMD]) or 451.3 g/kg enzyme‐treated soy (enzyme‐treated soybean meal diet [ESBMD]). All three test diets were supplemented with 38 g/kg of PM. Diets were fed based on a percentage of bodyweight adjusted after sampling the fish every 2 weeks to triplicate groups of Florida pompano juveniles (mean weight 8.06 ± 0.22 g). After 8 weeks of feeding, fish fed CPCD and ESBMD performed equally well in terms of final body weight, thermal growth coefficient, and percentage weight gain in comparison to fish fed PBMD. In all cases, feeding FSBMD resulted in poor feed conversion and lower feed intake compared to other treatments. Protein retention efficiency, whole‐body proximate composition, phosphorus, sulfur, potassium, magnesium, calcium, sodium, and zinc contents were not significantly influenced by the dietary treatments. The results obtained in the present histological study showed no significant differences in the thickness of serous layer, muscular layer, and submucosal layer of the intestine among treatments. Fish fed CPCD showed a significant widening of the lamina propria with an increase of cellular infiltration and higher presence of goblet cells compared to other dietary treatment. Based on these results, 451 g/kg ESBM or combination of 150 g/kg of CPC and 495 g/kg SBM supplemented with 38 g/kg PM can be utilized to develop a practical diet for juvenile Florida pompano without impacting growth, nutritive parameters, and several distal intestine health parameters. 相似文献
126.
还原糖含量是评价马铃薯全粉品质的重要指标之一,该文研究基于近红外光谱技术结合最小二乘支持向量机(least squares support vector machine,LSSVM)算法的马铃薯全粉还原糖含量非线性数学模型。采用移动窗口偏最小二乘法(moving windows partial least square,MWPLS)和连续投影算法(successive projections algorithm,SPA)组合方法筛选出20个特征变量,作为LSSVM的输入向量。优化径向基函数(radial basis function,RBF)的惩罚因子和核参数,训练LSSVM校正模型。经比较,LSSVM校正模型预测结果最优,预测相关系数为0.984,预测标准差为0.223%,相对分析误差(standard deviation ratio,SDR)为5.62。结果表明:近红外光谱结合LSSVM算法提高了马铃薯全粉还原糖含量的预测精度。 相似文献
127.
Pengfei Shi Jie Xu Xin Zhao Penglei Shen Dongmei Wen Qing Yu Yanfei Deng Deshun Shi Fenghua Lu 《Reproduction in domestic animals》2019,54(8):1104-1112
The objectives of present study were to evaluate the effect of casein kinase 1 (CK1) inhibition D4476 on in vitro maturation (IVM) and developmental competence of bovine oocytes. The cumulus oocyte complexes (COCs) were cultured in maturation medium with D4476 (0, 2, 5, 10, 20 μM) for 24 hr. After IVM and in vitro fertilization, through expansion average scores of cumulus cells (CCs), oocyte maturation efficiency, cleavage rate and blastocyst rate of zygote, we found 5 μM D4476 could increase the development potential of oocytes. After the COCs were treated with 5 μM D4476, the results of quantitative real‐time PCR analysis, Lichen red staining and PI staining showed that under without affecting germinal vesicle breakdown and nuclear morphology, D4476 could significantly decrease CK1 and upregulate TCF‐4 in oocytes. Furthermore, without influencing the level of Bad and CTSB, D4476 could significantly increase the expression of β‐catenin, TCF‐4, Cx43, MAPK, PTGS‐2, PTX‐3, TGS‐6, Bax and Bcl‐2 in CCs. Western blot analysis revealed that the addition of 5 μM D4476 during the maturation of COCs resulted in a lower level of Cx43 protein at 12 hr and a higher expression of Cx43 protein at 24 hr compared to the group without D4476. These results indicate that adding optimum D4476 (5 μM) to maturation medium is beneficial to maturity efficiency and development competence of bovine oocytes. 相似文献
128.
Abstract The objective of this study was to investigate the influence of different nitrogen fertiliser application levels in combination with different defoliation intervals on the dry matter (DM) production and water‐use efficiency of ryegrass (Lolium multiflorum,) cv. Midmar. In a field trial, four nitrogen levels (0, 150, 300 and 450 kg N ha?1) were combined with five defoliation intervals (every 2, 4, 6 and 8 weeks and twice a season). Soil moisture levels were measured with a neutron hydroprobe and were used to schedule irrigation. The dry matter production, nitrogen‐use efficiency (NUE), plant DM content and water‐use efficiency were influenced (P<0.01) by nitrogen level, defoliation interval, and the interaction between these factors. Total DM production varied from 64 to 20 922 kg DM ha?1. Dry matter production per unit of applied N decreased with an increase in N level within a defoliation interval. The plant DM content varied from 14.0% to 35.6%. The plant DM content decreased (P<0.05) with increasing N applications, while a lengthening of the defoliation interval resulted in an increase (P<0.05) in the DM content. No difference (P>0.05) was found in total water used when plants received N. Water‐use efficiency varied from 1.2 to 25.8 kg DM ha?1 mm?1 water received. 相似文献
129.
Detection of additive and additive×environment interaction effects of QTLs for yield‐component traits of rice using single‐segment substitution lines (SSSLs) 下载免费PDF全文
Additive effects (A) and additive‐by‐environment interactions (A×E) for five rice yield components were analysed using 20 SSSLs under mixed linear model methodology. Thirty‐one QTLs were detected. Different yield components have different QTL‐by‐environment (Q×E) interaction patterns. No A×E interaction effects were detected for the four QTLs for panicle number (PN). Four QTLs detected for spikelets per panicle (SPP) had A×E interactions. Five of seven QTLs detected for grains per panicle (GPP), two of 10 QTLs detected for 1000‐grains weight (GWT) and three of six QTLs detected for seed set ratio (SSR) showed significant A×E interaction. Most of these QTLs were distributed in clusters across the genome. The complexity of linkage and pleiotropy of these QTLs plus environmental effect may result in the diversity of the yield phenotype in the SSSLs. Only S19 exhibited a significant increase in yield with a predicted gain by 281.58 kg ha?1. The results may be useful to design a better breeding strategy that takes advantage of QTL‐by‐environment interaction effects in each of the SSSLs. 相似文献
130.
介绍了一种新的测量木材微纤丝角的无损检测技术--近红外光谱分析,并详细阐述了测量时木材样品的选择及制备,测量工作包括X射线衍射和近红外光谱采集、多变量数据分析与模型建立的方法及步骤.证明了近红外光谱分析技术可以用于快速准确地预测木材的微纤丝角. 相似文献