黄瓜游离小孢子培养诱导成胚和植株再生

 以10个不同基因型黄瓜为试材进行游离小孢子培养,通过对成胚条件的系统研究,从‘7447’和‘Poinsett97’中获得了子叶形胚和再生植株。研究结果表明：基因型和小孢子发育时期是限制黄瓜游离小孢子成胚的关键因子。不同品种间胚状体产量差异显著,每皿产胚1.5～33.4个。单核靠边期是进行黄瓜游离小孢子培养的最佳时期。低温预处理有利于胚状体的诱导,4 ℃预处理2～4 d为宜,以处理2 d时胚状体产量最高。外源激素在诱导胚状体时并非必需,但低浓度的外源激素（0.5 mg·L^-1 2,4－D、0.2 mg·L^-1 6－BA）更能促进小孢子成胚。NLN和B5两种基础培养基在诱导胚状体上无显著差异。子叶形胚易分化成苗,而其它类型的胚状体不能获得再生植株。     

Transpositional feeding rhythm of loach <Emphasis Type="Italic">Misgurnus anguillicaudatus</Emphasis> from larvae to juveniles and its ontogenesis under artificial rearing conditions

The diel feeding rhythm and ontogenesis during early life stage of loach Misgurnus anguillicaudatus were investigated under experimental conditions (light: L 06:00–18:00, D 18:00–06:00 h). Morphological and behavioral developments of loach from newly hatched to 40 days after hatching were observed. Larvae were able to prey on daphnia 3–4 days after hatching at 23 ± 0.5°C. As the larvae grew, they showed an increasing feeding capacity and a distinct feeding rhythm. Feeding intensity and incidence for day-4 larvae were highest at 10:00 and 16:00 h. The highest levels of feeding intensity for day-12 larvae occurred at 08:00, 12:00, and 18:00 h as did feeding incidence. By day 20, when the larvae metamorphosed, the highest levels of feeding intensity occurred at 06:00, 18:00, and 24:00 h and were concurrent with the highest feeding incidence. After metamorphosis, feeding capacity had again increased considerably and, in contrast to the earlier stages before day 20, feeding intensity for day-30 juveniles peaked at 05:00 and 20:00 h, about 1–2 h after the maximum feeding incidence. The feeding rhythm of loach juveniles at day 40 was almost the same as the day-30 juveniles. The estimated maximum daily feeding rates were 43.1%, 33.4%, 19.0%, 12.8%, and 5.8% of body weight on days 4, 12, 20, 30, and 40, respectively. Thus, loach was found to have different feeding rhythms in the pre- and post-metamorphosis stages, with the highest feeding activity in daytime during the larval planktonic stage before metamorphosis, and intensely nocturnal feeding behavior during the juvenile benthic stage after metamorphosis.     

氮肥后移对玉米冠层内物质分配及氮素利用的影响

研究不同施氮量、不同施肥时期对玉米产量与氮素利用的影响,对玉米干物质生产、氮素积累与分配进行分析。结果表明,施氮量为180 kg/hm2时,产量表现为基施处理最高,较拔节期、大喇叭口期施肥处理分别提高1.17%、7.69%;施氮量为75 kg/hm2时,拔节期施肥处理产量最高,较基施、大喇叭口期施肥处理分别提高10.31%、4.61%。大喇叭口期施肥处理的干物质积累与氮素积累量少,氮肥利用效率低,说明氮肥后移需考虑植株前期对氮素的需要。拔节期施肥处理产量与基施处理产量和氮素积累量相当或提高,植株干物质积累增加,冠层内茎叶干物质比例协调,生育后期穗下层茎叶干物重下降缓慢,氮素利用效率高。施氮量为75 kg/hm2,氮肥适当后移可提高氮肥利用率。     

利用隶属函数值法对玉米成熟期抗旱性的综合评价

以10个不同基因型玉米为试验材料,在宁夏中部干旱带,通过测定不同品种成熟期的K~+/Na~+、光合气体交换参数、农艺性状及产量性状等指标进行抗旱性鉴定,并利用主成分分析和隶属函数法对其抗旱性强弱进行综合评价。结果表明,干旱胁迫地和对照田中,各参试品种的光合特征参数、K~+/Na~+及形态性状等均有明显差异,且干旱胁迫条件下的净光合速率(P_n)、株高、产量均显著低于对照(P0.05)。同一品种不同器官的K~+/Na~+值表现为茎基茎叶根,表明植株体内的离子吸收和运输具有选择性,茎基具有贮存K~+的作用,根具有贮存Na~+的功能。不同基因型玉米品种的抗旱能力根据综合评价值(D值)大小,综合评价其抗旱性强弱顺序为A111-3/H242HoViY1北21/A58A3/A18高WY2宁3/1522H237/A18桂青贮1号H237/1506A3/A18。     

杂交中稻分蘖期干旱对产量的影响及其缓解技术研究

以杂交中籼迟熟组合宜香优300和川谷优642为材料,在海南陵水旱季大田条件下,研究了分蘖期干旱处理对中籼迟熟杂交稻产量的影响及其缓解技术。结果表明,干旱会造成水稻减产,主要原因是有效穗数和穗粒数显著降低;干旱期间平均田间持水量可作为预测产量损失度的依据;分蘖期干旱处理下,稻谷产量随着施氮量和移栽密度的增加而增加,适当提高本田施氮水平和移栽密度,可显著降低分蘖期干旱的产量损失度。     

花前氮亏缺对水稻叶片氮代谢酶活性的影响

以籼型杂交稻中浙优1号和籼粳型杂交稻甬优12为材料,试验分析了花前不同时期氮亏缺处理对水稻叶片氮代谢酶活性的影响。结果表明,花前氮亏缺导致植株上3叶氮浓度、NR、GS、GOGAT、GOT和GPT酶活性大幅下降,GDH活性显著增长,且各叶位对花前氮亏缺敏感度总体上表现为剑叶倒2叶倒3叶;破口期亏氮对籼型杂交稻中浙优1号上3叶氮同化酶活性的影响远小于减数分裂期处理,与之相比,籼粳杂交稻甬优12对破口期亏氮胁迫仍较敏感,表明中浙优1号植株氮代谢酶的亏氮敏感性由减数分裂期至破口期逐步下降,对土壤速效氮的需求同步降低,甬优12则对土壤供氮存在更高需求。     

Carbohydrate and crude protein fractions in perennial ryegrass as affected by defoliation frequency and nitrogen application rate

The objective of this study was to evaluate the effects of defoliation frequency (either at two‐ or three‐leaf stage) and nitrogen (N) application rate (0, 75, 150, 300, 450 kg N ha^?1 year^?1) on herbage carbohydrate and crude protein (CP) fractions, and the water‐soluble carbohydrate‐to‐protein ratio (WSC:CP) in perennial ryegrass swards. Crude protein fractions were analysed according to the Cornell carbohydrate and protein system. Carbohydrate fractions were analysed by ultra‐high‐performance liquid chromatography. Sward defoliation at two‐leaf stage increased the total CP, reduced the buffer‐soluble CP fractions and decreased carbohydrate fractions of herbage (P < 0·001). The effect of defoliation frequency was less marked during early spring and autumn (P < 0·001) than for the rest of the seasons. An increase in N application rate was negatively associated with WSC, fructans and neutral detergent fibre (P < 0·001), and positively associated with CP and nitrate (N‐NO₃) contents of herbage. Nitrogen application rate did not affect CP fractions of herbage (P > 0·05). The fluctuations in CP and WSC contents of herbage resulted in lower WSC:CP ratios during early spring and autumn (0·45:1 and 0·75:1 respectively) than in late spring (1·11:1). The herbage WSC:CP ratio was greater (P < 0·001) at the three‐leaf than the two‐leaf defoliation stage and declined as the N application increased in all seasons (P < 0·001). The results of this study indicate that CP and carbohydrate fractions of herbage can be manipulated by sward defoliation frequency and N application rate. The magnitude of these effects, however, may vary with the season.     

采收成熟度对烟叶烤后质量的影响

为明确密集烘烤条件下浓香型烤烟各部位烟叶成熟采收标准,通过对‘粤烟98’品种烟叶外观特性、化学成分感官质量评价,探究其在不同采收成熟度对烟叶品质的影响。结果表明:在韶关烟区烤烟烟叶以下、中、上部达到适熟期采收最佳,烤后烟叶橘黄烟比例增加,色泽光亮、色度强及油分好,各部位烟叶化学成分糖碱比和氮碱比协调性相对较好,香气质好量足,在一定程度上彰显了烟叶浓香型特征。     

水、旱条件下小麦苗期部分农艺性状的遗传分析

为研究水分胁迫对小麦幼苗部分农艺性状的影响,分析这些性状与干旱胁迫的关系。以‘青麦6号’和‘烟农24’杂交形成的F2:3群体为材料,采用主基因+多基因混合遗传模型对其进行遗传分析。水、旱2种条件下,根长和根鲜重均为1对加性-显性效应主基因模型;茎鲜重、茎干重、根干重、根茎鲜重比和根茎干重比的最适遗传模型不同。干旱胁迫条件下,茎鲜重主基因遗传率为37%,茎干重、根长、根鲜重、根干重、根茎鲜重比及根茎干重比主基因遗传率分别是51%、81%、74%、56%、50%、68%。茎鲜重、茎干重、根干重等性状,尽管检测到主基因,但在水旱条件下遗传模型不同,可进一步研究其遗传规律。     

细胞穿透肽介导的小黑麦小孢子遗传转化研究

本研究选用小黑麦小孢子作为供试材料,进行细胞穿透肽介导的植物单倍体细胞遗传转化可行性研究。提取单核靠边期的小黑麦小孢子进行悬浮培养,将细胞穿透肽与报告基因GFP的表达框复合物对小孢子进行转化。转化植株进行了PCR分子检测,并在荧光体视显微镜下观察外源GFP基因的表达情况。共获得21株候选转基因植株,其中染色体自然加倍植株为10株。T1代加倍植株的PCR检测结果显示,加倍的转基因株系中有3个株系的PCR检测结果呈阳性。通过荧光体视显微镜可在转基因植株的幼胚部位观测到GFP蛋白的荧光。以上结果表明外源GFP基因已经整合到小黑麦基因组上并且得到表达并稳定遗传。因此,由细胞穿透肽介导的植物单细胞转化是一种有效、可行的植物单细胞转基因方法。