黄瓜游离小孢子培养诱导成胚和植株再生

 以10个不同基因型黄瓜为试材进行游离小孢子培养,通过对成胚条件的系统研究,从‘7447’和‘Poinsett97’中获得了子叶形胚和再生植株。研究结果表明：基因型和小孢子发育时期是限制黄瓜游离小孢子成胚的关键因子。不同品种间胚状体产量差异显著,每皿产胚1.5～33.4个。单核靠边期是进行黄瓜游离小孢子培养的最佳时期。低温预处理有利于胚状体的诱导,4 ℃预处理2～4 d为宜,以处理2 d时胚状体产量最高。外源激素在诱导胚状体时并非必需,但低浓度的外源激素（0.5 mg·L^-1 2,4－D、0.2 mg·L^-1 6－BA）更能促进小孢子成胚。NLN和B5两种基础培养基在诱导胚状体上无显著差异。子叶形胚易分化成苗,而其它类型的胚状体不能获得再生植株。     

Transpositional feeding rhythm of loach <Emphasis Type="Italic">Misgurnus anguillicaudatus</Emphasis> from larvae to juveniles and its ontogenesis under artificial rearing conditions

The diel feeding rhythm and ontogenesis during early life stage of loach Misgurnus anguillicaudatus were investigated under experimental conditions (light: L 06:00–18:00, D 18:00–06:00 h). Morphological and behavioral developments of loach from newly hatched to 40 days after hatching were observed. Larvae were able to prey on daphnia 3–4 days after hatching at 23 ± 0.5°C. As the larvae grew, they showed an increasing feeding capacity and a distinct feeding rhythm. Feeding intensity and incidence for day-4 larvae were highest at 10:00 and 16:00 h. The highest levels of feeding intensity for day-12 larvae occurred at 08:00, 12:00, and 18:00 h as did feeding incidence. By day 20, when the larvae metamorphosed, the highest levels of feeding intensity occurred at 06:00, 18:00, and 24:00 h and were concurrent with the highest feeding incidence. After metamorphosis, feeding capacity had again increased considerably and, in contrast to the earlier stages before day 20, feeding intensity for day-30 juveniles peaked at 05:00 and 20:00 h, about 1–2 h after the maximum feeding incidence. The feeding rhythm of loach juveniles at day 40 was almost the same as the day-30 juveniles. The estimated maximum daily feeding rates were 43.1%, 33.4%, 19.0%, 12.8%, and 5.8% of body weight on days 4, 12, 20, 30, and 40, respectively. Thus, loach was found to have different feeding rhythms in the pre- and post-metamorphosis stages, with the highest feeding activity in daytime during the larval planktonic stage before metamorphosis, and intensely nocturnal feeding behavior during the juvenile benthic stage after metamorphosis.     

8种木兰科树种苗期在呈贡的适应性研究

从2004年至2009年在昆明市呈贡县进行了绒叶含笑、马关木莲、状丽含笑、香木莲、小果含笑、大果木莲、金花含笑、单性木兰8种木兰科树种的苗期适应性研究。5年多的育苗试验结果表明:单性木兰、金花含笑、大果木莲为该地区不适宜育苗用的木兰科树种。现存的5个木兰科树种苗木的保存率顺序是:绒叶含笑香木莲状丽含笑马关木莲小果含笑。适应性差或抗寒力弱的单性木兰等3个树种的苗木已在逐年的霜冻中被冻死。现存树种是苗期抗寒力较强的树种,其苗木的抗寒力的排序是:绒叶含笑马关木莲小果含笑状丽含笑香木莲。由于树种间苗木的抗寒力差异和生长差异,现存5个木兰科树种苗木的主要生长指标排序是:绒叶含笑马关木莲状丽含笑香木莲小果含笑。绒叶含笑与各树种间其苗木的苗高生长均具有极显著差异或显著差异,地径生长差异也较大;故绒叶含笑是昆明市园林、庭园绿化的一个首选树种,其次是马关木莲、状丽含笑。     

不同小麦品种(系)抗倒伏性状多样性分析

为了综合评价不同小麦材料的不同生育时期抗倒性,明确影响小麦倒伏的关键因素,以147份我国育成的主推小麦品种及新育成的小麦品种（系）为研究对象,在开花期、灌浆期和乳熟期测定其10个倒伏性相关性状,并利用主成分分析和聚类分析法对这些小麦材料抗倒性进行综合评价。结果表明,10个抗倒伏相关性状在不同基因型间、气候因素间及生育时期间均存在显著的变异;在不同生育时期,性状间的相关性基本一致,均表现为基部茎节壁厚度、实心度、茎秆强度对小麦的抗倒伏性具有一定的积极作用,株高、重心高度、基部茎节长度、髓腔直径具有一定的消极作用。主成分分析显示,茎秆强度是影响植株抗倒性的最关键性状。基于三个生育时期主成分的综合因子得分的聚类分析显示,有70份材料的综合抗倒性优良,其中部分新育成小麦材料抗倒性表现优异,可作为小麦强秆抗倒改良育种资源使用。     

夏大豆籽粒成熟期根叶衰老特性的研究

以在冀中南地区夏播表现相对高产和低产的6个大豆品种为材料,通过测定鼓粒始期至成熟期根系和叶片的生理性状,探讨了大豆籽粒成熟期根、叶衰老之间的关系。结果表明:根、叶生理参数之间高度相关,不同产量类型大豆品种根系可溶蛋白含量、可溶糖含量、SOD活性衰退幅度均小于叶片,表明在生育后期叶片衰老先于根系;不同产量类型间比较,相对高产品种较相对低产品种根、叶各生理指标衰退速率慢,相对高产品种根系较叶片衰老的进程显著落后于相对低产品种。应在栽培上重视养根护叶及育种中加强根系生理性状的选择,延缓衰老进程进而获得高产。     

小麦灌浆中期旗叶黄化速率与产量性状及部分品质性状的关系

为了给高产、优质小麦育种和栽培提供参考依据,以50个小麦基因型为试验材料,研究不同小麦基因型间灌浆中期旗叶黄化速率的差异及其与产量性状和部分品质指标的关系。结果表明：（1）不同小麦基因型间灌浆中期旗叶黄化速率差异极显著;（2）灌浆中期旗叶黄化速率与穗粒数、千粒重和穗粒重呈负相关,相关系数（R）分别为-0.171、-0.405^**和-0.369^**;（3）灌浆中期旗叶黄化速率与蛋白质含量、湿面筋含量和沉降值的相关系数（R）分别为-0.085、-0.111和-0.045,相关不显著。     

大豆品种鼓粒期田间抗旱鉴定

大豆鼓粒期缺水是制约大豆产量形成的重要因素之一。在鼓粒期对大豆品种进行抗旱性鉴定,可为大豆抗旱育种和抗旱节水栽培提供依据。选取不同地区育成的代表性品种（品系）46份,在鼓粒期田间干旱与灌水条件下,分析其产量、产量性状、生育期与形态性状、籽粒品质性状等指标,并且对其进行抗旱性鉴定。结果表明,干旱区46份材料平均产量1 759.6 kg/hm²,灌水对照区平均产量2 223.1 kg/hm²,46个品种平均耗水系数1.079 t/kg。干旱对三粒荚影响显著,对单株荚数、单株粒数、生物产量、单株粒重、百粒重影响极显著。干旱区的平均蛋白质含量极显著高于灌水区,比对照区提高幅度4.47 %;平均脂肪含量极显著低于灌水区,比正常灌水区降低幅度4.19 %。干旱条件下品种百粒重下降,生育期提早均属于品种抗旱性的适应反映,与抗旱性无关。适于呼伦贝尔的品种推荐为北豆14、黑河50、黑河43、华疆3、登科1号、蒙豆15、北豆37、黑河36、北豆38等。     

春季氮肥和多效唑调控对小麦生育和产量的影响

为明确春季调控措施对冬小麦生长发育和籽粒产量的影响,以冬小麦品种石新828为材料进行田间试验,4个处理分别为:起身期追全部氮肥(除基肥外,下同)并叶面喷多效唑(N1);起身期追2/3氮肥并喷多效唑+拔节期追1/3氮肥(N2);起身期追1/3氮肥+拔节期追2/3氮肥(N3);拔节期追全部氮肥(N4)。生育期间测定群体和个体生育特性,成熟期调查产量性状。结果表明,N1和N2处理小麦拔节期的总茎数、叶面积指数(LAI)和干物质积累量均显著高于N4和N3处理。孕穗期N4和N3处理小麦的总茎数、LAI和干物质积累量显著高于N1和N2处理。开花到成熟期各处理的总茎数、LAI和干物质积累量差异均不显著。孕穗期前,不同处理的株高差异不显著,孕穗期后,N4处理的株高最高,且显著高于N1处理。各处理基部节间直径和中上部节间长的差异不显著,N4处理基部第一节间长度显著大于其他处理。随追氮时期前移或前期施氮量增多,不孕小穗数减少,结实小穗和穗粒数增加,N1比N4处理不孕小穗数显著减少,结实小穗和穗粒数显著增多。N1处理小麦成熟期的千粒重最高,且显著高于N4处理。N1处理的籽粒产量最高,且显著高于N3和N4处理。起身期追氮配合多效唑调控,可以获得比拔节期追氮更高的穗粒数和千粒重,从而获得更高的产量。     

Effects of Exogenous Materials on Nitrogen Photosynthetic Efficiency and Yield Formation of japonica Rice in Cold Region Under Cold Water Stress at Booting Stage

【Objective】Our goal is to develop disaster-reducing, production-preserving and chemical-controlling cultivation techniques to effectively resist cold damage at the booting stage of japonica rice in cold region.【Method】The split plot design and the central combination test design were used to screen out the optimal application concentrations of CaCl2, glutamic acid(Glu), and γ-aminobutyric acid(GABA) through the membership function values of yield-related indicators under cold-water stress at booting stage.【Result】1) Under cold water stress at the booting stage, the optimal concentrations of exogenous CaCl2, Glu and GABA (CGG) were 1.87 mmol/L, 2.76 mg/L, 4.40 mmol/L, respectively and the average membership function value is the largest (0.932). 2) Compared with normal irrigation, the nitrogen photosynthetic efficiency of cold-region japonica rice decreased under cold stress at the booting stage. Compared with cold water stress at the booting stage, CGG application at the optimal concentration significantly increased the leaf area index (5.37%) and nitrogen photosynthetic efficiency (4.53%) of Dongnong 428 at the full heading stage; significantly increased leaf biomass per leaf area (2.56%) and leaf area index (3.39%) of Songjing 10 at the full heading stage. 3) Compared with cold water stress at the booting stage, the application of the optimal concentration of CGG increased the dry matter accumulation from heading stage to maturity in the cold-region japonica rice, but it was still lower than that of the normal irrigation, and there were differences between varieties and treatments. 4) Compared with cold water stress at booting stage, the yield and yield components of cold-region japonica rice increased with the application of the optimal concentration CGG, and the impact on cold-sensitive varieties was more significant. 5) Compared with the cold water stress at the booting stage, the average increase in temperature production efficiency of Dongnong 428 and Songjing 10 was 5.55% and 23.72% under the optimal concentrations of CGG, respectively. The contribution rate of exogenous CGG to the gap of temperature production efficiency is 8.99%.【Conclusion】The application of the optimal concentration of CGG under cold water stress at the booting stage promoted leaf growth of cold-region japonica rice at the full heading stage and increased the population nitrogen photosynthetic efficiency, which was beneficial to dry matter accumulation and yield formation after heading stage, thereby reducing the loss of cold-region japonica rice under cold water stress at booting stage and improving the temperature production efficiency of cold-region japonica rice.     

灌浆成熟期温度对水稻籽粒淀粉合成关键酶活性及品质的影响

 选用蒸煮食味品质不同的3个粳稻品种，利用温室高温和自然常温，研究了灌浆成熟期温度对水稻籽粒淀粉合成关键酶活性及蒸煮食味品质的影响。结果表明，灌浆成熟期高温条件下，水稻籽粒蛋白质含量提高，直链淀粉含量和味度值降低，劣质品种提高或降低的程度大于优质品种；RVA谱特性对灌浆成熟期温度的反应因品种和特性而异；灌浆过程中，籽粒ADPG焦磷酸化酶、可溶性淀粉合成酶和淀粉分支酶活性随灌浆进程，酶活性逐渐增加，达到峰值以后，酶活性又逐渐下降，呈单峰曲线，但不同品种酶活性达到峰值的时间和同一时期的酶活性大小有差异；ADPG焦磷酸化酶和可溶性淀粉合成酶的活性表现对温度的影响反应较为迟钝，而淀粉分支酶活性表现对温度变化的反应较为敏感，温度过高过低都降低该酶活性。