Management Information System of Power Based on C/S and B/S Mode Combined

After analyzing the feature of C/S and B/S modes, a kind of MIS for power industry, which combined C/S with B/S, is put forward. The C/ S platform is developed with the Powerbuilder 7.0 and SQL Server 7.0, and the B/ S platform is developed with ASP and IIS5.0+ SQL Server7.0. Electric power management information system should be constituted by the seventh statures mold piece, which are the electricity measures to collecting, electricity charges settling accounting system, the safety system et al. In order to meet the demand of data safety of enterprises, the method of passing data between C/S and B/S is introduced.     

秋季叶施尿素及乙烯利对枣树贮藏氮素的影响

枣树(Zizyphus jujuba Mill.)秋季叶施尿素、乙烯利及尿素十乙烯利均可促进叶片及落性枝的氮素向树体回流,增加树体贮藏氮素水平,尤以尿素 200ppm乙烯利的效果最佳;尿素及乙烯利处理均增加了次年枣吊长度、平均单果重及枣果还原糖含量;尿素处理还显著增加了叶片及新梢含氮量,座果数,但降低了枣果蔗糖含量。枣树秋冬季地上部与根系具有同等重要的贮藏氮素作用;蛋白态氮是主要贮藏形态,为非蛋白态氮的2—3倍。     

微机多功能汉字人事管理系统的研制

本系统是一个包括数据维护、报表形成、信息检索、数值统计和系统维护等子系统在内的集成软件,是一套可同时处理人事、组织、劳资、财务、教务等综合信息多功能系统,适用于 IBM—PC 系列和长城系列微机及全兼容机,处理对象为 dBASEⅢ—PLUS 数据库,使用对象为各企事业人事、劳资等部门管理人员。系统功能完备,操作简单,菜单提示,汉字对话,采用树形结构化模块制,每底层节点均于屏幕一角开提示窗口,使用方便,易于推广。配有动态汉字显示方式,多色彩,多方向,夹带音乐提示,使用户有良好心理反应,减少视觉疲劳。     

Prolonged elevated atmospheric CO2 does not affect decomposition of plant material

Prolonged elevated atmospheric CO₂ might alter decomposition. In a 90-day incubation study, we determined the long-term (9 years) impact of elevated CO₂ on N mineralization of Lolium perenne and Trifolium repens plant material grown at ambient and elevated CO₂ and low- and high-¹⁵N fertilizer additions. No significant differences were observed in recovery rates between any of the treatments, except an N addition effect was observed for L. perenne (0.4 versus 0.5% day⁻¹ in high versus low N). The results suggest that elevated CO₂ did not change plant N mineralization in any of the soils, because of a surplus of available N in the fertilized and leguminous systems, and because of insignificant plant responses to elevated CO₂ in the low soil N availability systems.     

褐飞虱近似种伪褐飞虱和拟褐飞虱的寄主植物

 通过室内笼罩饲养法观察了褐飞虱\[Nilaparvata lugens (Stl)\]的两种同属近似种伪褐飞虱(N. muiri China)和拟褐飞虱\[N. bakeri (Muir)\]在水稻、游草\[Leersia hexandra (李氏禾)\]、秕谷草(L. sayanuka)等禾本科常见作物或杂草上的成虫繁殖力、卵和若虫发育情况。结果表明,伪褐飞虱、拟褐飞虱的适宜寄主不同于褐飞虱,前两者为游草和秕谷草,褐飞虱则为水稻。水稻上的伪褐飞虱和拟褐飞虱均能完成世代发育,但种群趋势指数分别仅0.2和0.02,不能持续繁衍。3种褐飞虱的繁衍能力有明显差别,褐飞虱明显高于伪褐飞虱和拟褐飞虱,三者的最高种群趋势指数分别为水稻上褐飞虱的333.82、秕谷草上伪褐飞虱的82.06和游草上拟褐飞虱的39.28。     

SREBP-1c基因重组腺病毒的构建及其在犊牛原代肝细胞中的表达

将携带有化学合成的SREBP-1c基因的质粒先用Xba Ⅰ酶切,Klenow补平突出末端,纯化回收后再用Hind Ⅲ酶切,切胶回收目的基因片段;并将其克隆至用EcoR Ⅴ/HindⅢ酶切回收的pShuttle-EGFP-CMV(-)TEMP穿梭载体上.经酶切鉴定后,重组穿梭质粒和腺病毒pAdxsi质粒分别用I-Ceu Ⅰ+I-Sce Ⅰ双酶切处理,T4DNA连接酶连接,获得重组质粒pAdxsi-GFP-SREBP1c,酶切鉴定后经Pac Ⅰ酶切线性化转染HEK293细胞,经包装扩增后用TCID50测定病毒滴度.以重组腺病毒感染犊牛原代肝细胞,采用qRT-PCR和Western blot检测细胞中SREBP1c mRNA和蛋白的表达量.结果显示,重组腺病毒酶切鉴定正确,滴度可达1.2×1010,腺病毒感染犊牛原代肝细胞中SREBP-1c mRNA和蛋白的表达显著升高.结果表明,本试验成功构建了SREBP-1c基因过表达重组腺病毒,且SREBP-1c在犊牛原代肝细胞中高度表达.     

西洋参种植土壤重金属含量变化

为研究西洋参种植过程中土壤重金属含量的变化,选取了文登地区西洋参种植1~4 年 的土壤作为样品,对样品中的重金属含量进行测定。结果表明:土壤重金属含量与种植年份之 间没有相关性,符合《土壤环境质量标准农用地土壤污染风险管控标准（试行）》。     

原花青素A-1体内外对猪与小鼠T淋巴细胞亚群的影响

为研究从碎米花杜鹃叶中分离得到的化合物原花青素A-1（Proanthocyanidin A-1，简称PAA-1）的免疫增强活性，初步从细胞水平上探讨其免疫增强机制。体外试验采用流式细胞术，检测PAA-1体外对小鼠脾淋巴细胞T细胞亚群CD4和CD8单阳性和双阳性T淋巴细胞亚群百分率及CD4+/CD8+比值；体内试验通过饲喂不同剂量的PAA-1后，检测试验猪外周血淋巴细胞CD4和CD8单阳性以及CD4+/CD8+比值。结果证明，体外试验中同阴性对照组相比，PAA-1能单独或协同Con A提升具CD4+、CD8+及CD4+CD8+表型的T淋巴细胞亚群百分率，提高CD4+/CD8+的比值；体内试验中饲喂中、高剂量的PAA-1的试验猪外周血中具有CD4+、CD8+表型的T淋巴细胞的百分率及CD4+/CD8+的比值均有显著提高。说明PAA-1可通过增加辅助性T淋巴细胞和细胞毒性T淋巴细胞数量、促进T淋巴细胞的成熟以及增加CD4+/CD8+的比值发挥增强细胞免疫的作用，为其进一步开发为新型免疫增强剂提供试验依据。     

The inhibitory effect of quercitrin gallate on iNOS expression induced by lipopolysaccharide in Balb/c mice

Quercetin 3-O-β-(2"-galloyl)-rhamnopyranoside (QGR) is a naturally occurring quercitrin gallate, which is a polyphenolic compound that was originally isolated from Persicaria lapathifolia (Polygonaceae). QGR has been shown to have an inhibitory effect on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Therefore, this study was conducted to investigate the inhibitory effect of QGR on nitric oxide production and inducible nitric oxide synthases (iNOS) expression in LPS-stimulated Balb/c mice. To accomplish this, 10 mg/kg of QGR was administered via gavage once a day for 3 days. iNOS was then induced by intraperitoneal injection of LPS. Six hours after the LPS treatment the animals were sacrificed under ether anethesia. The serum levels of NO were then measured to determine if QGR exerted an inhibitory effect on NO production in vivo. LPS induced an approximately 6 fold increase in the expression of NO. However, oral administration of QGR reduced the LPS induced increase in NO by half. Furthermore, RT-PCR and western blot analysis revealed that the increased levels of iNOS expression that occurred in response to treatment with LPS were significantly attenuated in response to QGR pretreatment. Histologically, LPS induced the infiltration of polymorphonuclear neutrophils in portal veins and sinusoids and caused the formation of a large number of necrotic cells; however, pretreatment with QGR attenuated these LPS induced effects. Taken together, these results indicate that QGR inhibits iNOS expression in vivo as well as in vitro and has antiinflammatory potentials.     

Cytokine response in Balb/c mice infected with Francisella tularensis LVS and the Pohang isolate

We investigated the immune response induced by the Francisella (F.) tularensis live vaccine strain (LVS) and the Pohang isolate. After the Balb/c mice were infected intradermally (i.d) with 2 × 10⁴ cfu of F. tularensis LVS and Pohang, respectively, their blood and organs were collected at different times; 0, 3, 6, 24, 72, 96, 120 and 168 h after infection. Using these samples, RT-PCR and ELISA analysis were carried out for the comparative study of the cytokines, including TNF-α, INF-γ, IL-2, IL-4, IL-10 and IL-12. In the Pohang-infected mice at 120 h, the liver showed a 53 times higher level of TNF-α and a 42 times higher level of IFN-γ than the respective levels at the early time points after infection. The levels of TNF-α and IFN-γ induced by LVS were 5 times lower than those induced by the Pohang isolate. Also, the organs from the Pohang-infected mice showed higher levels of TNF-α, IFN-γ, IL-10 and IL-12 than the levels in the LVS-infected mice. The blood from the Pohang-infected mice at 120 h revealed about a 40 times increased level of IFN-γ, and IL-10 was also increased by 4 times at 96 h compared to an early infection time point, while IL-4 was not induced during the whole infection period. These results suggest that F. tularensis may induce a Th1-mediated immune response to in vivo infection and the Pohang isolate has a higher capacity than the LVS to induce an acute immune response in Blab/c mice.