知识经济与高校图书馆发展

分析了知识经济的内涵、对社会经济发展的推动作用及其基本特征，探讨了知识经济时代的到来给高校图书馆带来的历史机遇与严峻挑战，提出了高校图书馆应作出的战略选择。     

Characterization of cytoplasmic male sterility in Petunia hybrida and Zea mays. Localization and activity of cytochrome c oxidase

Summary Anthers of male fertile, cytoplasmic male sterile (cms), and restored male fertile Petunia hybrida, are analyzed for cytochrome c oxidase (cox) activity in subsequent stages of microsporogenesis, and compared with anthers of male fertile, cms-S and cms-C Zea mays. The cox activity is determined in anther extracts and cytochemically. In petunia anthers, the first differences in cox activity occur from meiosis onward. However, at these stages, the initial symptoms of degeneration are already apparent. It is suggested that the decline in enzyme activity of the cms petunia anthers is the result rather than the cause of the non-formation of functional pollen.In maize anthers, the cox activity of sterile-type anthers is reduced in comparison with fertile-type anthers from premeiosis onward. There are also consistent cytochemical differences in the mitochondrial organization of cox activity between pollen of cms-S and male ferile maize anthers. In fertile-type mitochondria, the DAB reaction product indicating cox activity is localized in the cristae and within the space between the outer and inner limiting membranes of the organelles. In mitochondria of pollen of cms-S maize, cox activity is only observed between the outer and inner membranes of the mitochondria. The biochemical and cytochemical differences are observed at stages of development at which no structural signs of degeneration are apparent. The results suggest that cms in maize correlates with deviations in cytochrome c oxidase activity.Abbreviations cox cytochrome c oxidase - DAB 3,3-diaminobenzidine     

Cloning of vacuolar H<Superscript>+</Superscript>-ATPase subunit c genes from Japanese iris,and functional characterization in yeast

Five different isoforms (IrlVHA-c1-c5) of V-ATPase subunit c (VHA-c) were cloned from a Japanese iris (Iris lactea Pall. var. chinensis Fisch. Koidz) cDNA library using degenerate primers PCR and the 5'-RACE technique. The sequence analysis showed the open reading frame (ORF) of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a g...     

Little effect of forest age on oribatid mites on the bark of trees

This study investigates the effect of forest age (20-, 50-, 160-year-old, and primeval forest) on oribatid mite communities on the bark of oak trees in the National Park Pusza Białowieża in eastern Poland. We hypothesized that oribatid mite diversity on bark peaks at forests of intermediate age and that the number of parthenogenetic species of oribatid mites is highest in young stands. In contrast to these hypotheses, the diversity, density, number of juveniles, community structure and the mode of reproduction of oribatid mite species were not significantly affected by forest age. None of the oribatid mite species occurred exclusively on trees of a specific age. The results suggest that oribatid mite communities on the bark of trees are minimally affected by tree harvesting regimes. In contrast to oribatid mites in soil, communities on bark appear to be less sensitive to disturbances.     

家鹑与野生日本鸣鹑进化趋异水平的初步研究

 对微山湖野生日本鸣鹑、小型家鹑与中型家鹑在不同试验分组情况下的生态行为进行观察与统计 ,结果表明 ,家鹑与野鹑在体型和生物学习性上有所不同 ;野生与家养鹌鹑的杂交需经历由排斥到亲合的过程 ;公、母鹑均有较强的择偶性 ;野生鹌鹑与家鹌鹑可以成功地实现交配 ,也可以产生受精蛋 ,受精率却不高 ;2种家鹑与野生原种在形态、生态特性方面已形成了不同程度的分化     

均质和脱气对番茄沙司稠度和某些理化性状的影响

研究了均质和脱气对含淀粉的番茄沙司的稠度、色泽及可溶性固形物等理化性状的影响。测定了均质对其流变学特性的影响。结果表明，均质可增加番茄沙司的稠度，使可溶性固形物少量上升，而对色泽影响不大。脱气对含淀粉番茄沙司稠度的作用随淀粉含量和均质程度不同而异，对色泽、可溶性固形物影响不大。番茄沙司为典型的非牛顿流体，但当淀粉含量达０．５％和进行高速均质时，流变学曲线从弹性或假弹性流体变成膨胀流体。番茄沙司稠度三种测定方法之间存在显著的直线相关.     

布氏田鼠肝脏内线粒体蛋白含量及细胞色素c氧化酶活性的变化

为探讨线粒体能量代谢中细胞色素c氧化酶(cytochromecoxidase,CcO)在布氏田鼠(Microtusbrandti)生长发育过程中的作用,应用紫外分光光度法测定了不同日龄的幼鼠肝脏内线粒体蛋白含量及CcO活性。结果发现根据幼鼠肝脏内线粒体蛋白的含量可将生长发育大致分为3个阶段:1～3日龄,4～19日龄,20日龄至成体。CcO活性变化趋势则呈倒钟形,以17日龄的CcO活性为最高。据此推测布氏田鼠肝脏内CcO的活性可能与恒温机制的建立有关系。     

北京植物新资料

本文收录了<北京植物志>未收录的植物计4种,2变型.它们是白花大叶铁线莲、掌叶大黄、小叶羊角芹、丁香叶忍冬、多头苦荬菜.白花大叶铁线莲为新变型,羊角芹属与岩风属为北京新记录属.     

Effect of growth arrest-specific protein 6 on H9c2 cell apoptosis induced by anoxia-reoxygenation via PI3K/Akt survival pathway

AIM: To investigate the effect of growth arrest-specific protein 6(Gas 6) on H9c2 cell apoptosis induced by anoxia-reoxygenation (A/R) and its possible relationship with PI3K/Akt pathway. METHODS: Cultured H9c2 cell line of cardiomyocytes was randomly divided into 4 groups: normal control group, anoxia-reoxygenation group (A/R), anoxia-reoxygenation+Gas6 group (A/R+Gas6) and anoxia/reoxygenation+Gas6+LY294002 group (A/R+Gas6+LY294002). The procedure of A/R was performed in cultured H9c2 cells by 3 h of anoxia and then 3 h of reoxygenation. The viability of the cells and the activity of caspase-3 were detected by automatic biochemistry analytic instrument. Cell apoptotic rates were evaluated by flow cytometry. The protein level of phosphorylated Akt(p-Akt) was determined by Western blotting. RESULTS: Compared with control group, the cell viability was significantly decreased, and caspase-3 activity, cell apoptotic rate and the protein level of p-Akt were increased in A/R group. Compared with A/R group, the caspase-3 activity and cell apoptotic rate reduced markedly, while the cell viability and the protein level of p-Akt were significantly increased in A/R+Gas6 group .The effect of Gas6 was inhibited by LY294002. CONCLUSION: Gas6 may protect the H9c2 cells from anoxia-reoxygenation-induced apoptosis. Its mechanism is possibly involved in the activation of PI3K/Akt survival pathway via increasing the phosphorylation of Akt protein.     

小样本情况下需水量预测模型研究

针对传统的GM（1,1）模型,分析了其预测结果与实际过程存在的偏差,通过GM（1,1）模型与自回归滑动平均模型相结合的方法以弥补偏差。以钦州市1999-2009年及2005-2009年城市供水总量这两组基础数据对本文所建模型进行验证,并利用此模型预测了钦州市2010-2018年需水量。通过与其他预测模型的对比,进一步证...