小剂量肝素对危重新生儿伴全身炎症反应综合征的早期干预作用

目的观察小剂量肝素对危重新生儿全身炎症反应综合征(SIRS)的治疗效果及预后的影响。方法对我科2005年6月至2007年6月间符合诊断标准的94例新生儿SIRS患儿随机分组进行前瞻性对照研究,对照组46例采用常规治疗,治疗组48例在常规治疗基础上每次加用小剂量肝素(5u/kg)皮下注射,每6h 1次,连用3d,观察两组C-反应蛋白(CRP)、血小板计数(PLT)、D-二聚体(D-D)治疗前后的变化。结果治疗组SIRS持续时间>3d者16例(33.3%),明显少于对照组的28例(60.9%),MODS发生率和病死率也明显低于对照组(10.4%、4.2%vs34.8%、17.4%)(P<0.01或0.05);两组治疗后的PLT、CRP及D-D水平均明显改善,且以治疗组更为明显(P<0.01)。治疗组使用肝素后凝血指标均恢复正常或趋于正常。结论早期应用小剂量肝素可有效缩短SIRS持续时间,MODS的发生率及病死率明显降低。     

低分子肝素对高脂血症性胰腺炎大鼠VEGF表达的影响

目的 研究低分子肝素对大鼠高脂血症性胰腺炎胰腺微循环的作用机制。方法 将72只健康SD大鼠随机分成假手术组、模型组和治疗组各24只,每组再随机分成四个亚组。先采用高脂饮食建立高脂血症模型,模型组和治疗组制作大鼠急性胰腺炎模型,制模成功后治疗组分别于0、6、12、18 h开始使用低分子肝素皮下注射,各亚组均间隔6 h重复皮下注射一次,每亚组均干预4次;模型组和假手术组同时间点皮下注射等量生理盐水对照。结果 大鼠胰腺血流速度模型组低于假手术组,治疗组高于模型组（P<0.05）;胰腺组织病理学评分模型组高于假手术组,治疗组低于模型组（P<0.05）;模型组各亚组胰腺血管内皮生长因子（VEGF）蛋白表达、VEGF mRNA表达水平均高于假手术组,而治疗组均低于模型组（P<0.05）。结论 大鼠高脂血症性胰腺炎,VEGF与微循环障碍密切相关;低分子肝素可能通过下调胰腺组织VEGF水平来改善胰腺微循环障碍,减轻胰腺组织进一步损害,且早期干预价值更大。     

Effect of sample handling on venous PCO2, pH, bicarbonate, and base excess measured with a point-of-care analyzer

Objective: The purpose of this study was to determine the effect of timing of analysis, collection tube type and repeated opening of sample tubes on venous PCO₂, pH, HCO₃, and base excess (BE) results. Design: Prospective experimental study, paired sample analysis. Setting: Veterinary Medical Teaching Hospital. Animals: Twenty dogs. Interventions: Jugular venous blood samples. Measurements and main results: PCO₂, pH, HCO₃, and BE were determined immediately following collection (control) and at selected times up to 30 minutes after placement in either screw top or vacuum heparin collection tubes. A different set of screw top and vacuum heparin collection tubes were sampled repeatedly over time for up to 15 minutes. In the screw top delayed analysis group, only pH changed significantly at one time point. PCO₂ decreased significantly in all other groups and resulted in a significant reciprocal pH change in the vacuum tubes with either delayed single analysis or repeated sampling. HCO₃ and BE declined significantly in multi‐sampled vacuum tubes and HCO₃ also decreased significantly in multi‐sampled screw top tubes. Conclusions: Analysis of acid–base status is optimally performed on freshly drawn blood. However, when it is anticipated there will be a delay in analysis of samples kept at room temperature, the use of 2.0 mL plastic screw top heparin anticoagulant tubes may result in fewer pre‐analytical errors than 3.5 mL glass vacuum tubes.     

添加肝素对猪睾丸细胞增殖的影响

猪睾丸细胞（ST细胞）主要用于猪瘟等病毒的培养。猪睾丸细胞增殖试验结果表明,通过在常规培养基中添加肝素用来培养ST细胞,可有效增加细胞密度,改善细胞生长状态,有望为ST细胞工厂化转瓶生产提供指导依据。