Persistence and performance of esfenvalerate residues on broccoli

The efficacy of esfenvalerate (84 g litre-1 EC; Asana XL) at 7.0 g AI ha-1 on broccoli was tested against the flea beetle, Phyllotreta cruciferae Goeze (Chrysomelidae: Coleoptera) and the imported cabbage worm, Pieris rapae L (Pieridae: Lepidoptera) under field conditions. Insect populations were monitored before and after treatment. Periodic sweep-net collections and examination of the leaves in treated and untreated broccoli plots revealed mean reductions of P cruciferae levels of nearly 98% 1 week post-application compared with untreated plots. The residual toxicity of esfenvalerate was also effective for 2 weeks in reducing population density of P rapae by 69% on broccoli leaves. The impact of esfenvalerate on feeding damage to broccoli leaves was established by counting the number of feeding holes made by P cruciferae on spring broccoli and P rapae on fall broccoli. As the leaf area ingested increased, a linear relationship was seen between the number of holes and number of insects. Results indicated that forage destruction by the two pests was significantly reduced by esfenvalerate application. Esfenvalerate was extracted from broccoli at 1 h and 1, 3, 7, 10, and 14 days post-application for residue analysis. Residues on spring broccoli were 12.2, 5.2 and 2.9 micrograms cm-2 on the leaves and 0.13, 0.05 and 0.02 microgram g-1 on the heads at 1 h, 1 and 3 days, respectively. Only trace levels (0.001 microgram g-1) were detected in/on the heads 14 days after spraying. On the basis of half-life (T1/2) values, persistence of esfenvalerate on spring broccoli leaves (T1/2 = 1 day) was shorter than on fall broccoli (T1/2 = 1.6 days). T1/2 values were 2.1 and 3.6 days on spring and fall broccoli heads, respectively. The implications of these residue levels on re-entry times into treated fields are discussed.     

丁酸梭菌对肠道上皮细胞黏附及对鳗弧菌抑制的研究

本试验以鱼肠道上皮细胞为模型,以肠道的致病鳗弧菌为对照,研究了丁酸梭菌对鱼肠道上皮细胞的黏附作用及黏附对细胞的损伤和成活率的影响。结果表明∶丁酸梭菌对鱼的肠道上皮的黏附率为7.39±1.85,低于鳗弧菌的黏附率(P<0.05);对细胞的损伤率为1.02±0.35,显著低于鳗弧菌对细胞的损伤率(P<0.05);黏附后丁酸梭菌组对细胞的成活率的影响与正常对照组比较,差异不显著;当酪酸菌、鳗弧菌与肠道上皮细胞共同培养时,使鳗弧菌的黏附率下降7.03%(P<0.05)。因此,该株丁酸梭菌可以安全地黏附鱼肠道上皮细胞,并能有效抑制鳗弧菌的黏附。     

F-2毒素致体外培养大鼠睾丸支持细胞DNA损伤

为探讨F-2毒素对雄性生殖机能的影响,取大鼠睾丸支持细胞进行体外培养,运用单细胞凝胶电泳技术检测51、0、20和40 mg/L的F-2毒素攻毒后24 h支持细胞DNA的损伤情况。结果显示,F-2毒素在一定浓度范围内对体外培养的支持细胞DNA产生损伤作用,且受损程度随着F-2毒素攻毒剂量而升高,具有明显量效关系。除5 mg/L剂量组外,其余各组的细胞受损率、彗星尾长和DNA损伤程度与阴性对照组相比差异极显著（P〈0.01）,表明F-2毒素对体外培养的大鼠睾丸支持细胞有毒性作用,能够损伤细胞DNA。     

西北地区沙尘暴的危害及对策

该文以大量的例证,详实的数据介绍了西北地区沙尘暴的危害,并综合分析了沙尘暴产生形成原因、危害方式,为保护生态环境提出了治沙防沙的基本对策。     

浅谈孔隙率对沥青路面的影响

沥青混合料的孔隙率是一个重要的设计参数，其对于沥青路面的强度和使用性能有着重要的影响。在施工时，由于施工条件和设备的影响，不可能达到设计的孔隙率，于是一般要求用压实度作为施工时的一个控制指标，将沥青路面施工后的孔隙率控制在一个可接受的范围。在路面使用的初期，必然会有一段时间处于未完全压实工作状态，这种工作状态下，沥青路面能够提供的是未完全稳定的早期强度。在设计时，需要对比多个级配的混合料，确定合理的早期强度。     

沙漠地区农业种植技术初探

沙漠地区在没有造林或造林初期防护效益不显著情况下，农业种植受风沙危害比较严重，提高农业种植产量是我们面临的一道难题。本文对中国林科院沙漠林业实验中心井灌区第四实验场风沙环境和三年来的种植情况进行分析，得出农业防风害的措施有以下几个方面：农作物品种的选择；种植时间的调整；以增加起动风速为目的的各项抗风害措施的采用；以减小风沙流强度和目的的阻沙林和固沙工程的建造。这些措施能有效地减小和防止风沙灾害的发     

植物性抗生育剂防治幼林地兔害研究

在山西省吕梁山国有林管理局下李林场新造油松和侧柏混交林内,以20m×40m的面积投放一堆植物性抗生育剂和红胡萝卜混合药饵200g,对照地不放药。通过对施药前后试验地与对照地兔粪和苗木为害率调查数据进行统计分析,结果表明,与对照相比,投放植物性抗生育剂能显著减少幼林地内野兔活动频率和对苗木的为害。     

Splenectomy enhances sensitivity of islets to STZ in mice

AIM: To explore the protective role of spleen in damage of islet beta cells induced by streptozotocin (STZ).METHODS: Splenectomy in mice was performed by surgery. Sixty splenectomy mice were divided into 3 groups as the mice were intraperitoneally injected with STZ at doses of 80 mg/kg or 160 mg/kg, and saline, respectively. Sixty normal mice (without splenectomy) were also grouped and treated as above for controls. One week later, fasting blood glucose and serum insulin were monitored,and beta cell mass and the apoptosis of islet cells were analyzed by the methods of immunohistochemistry and ELISA, respectively. The content of reactive oxygen species was determined by the method of luminol-enhanced chemiluminescence.RESULTS: Compared to the normal control mice, the concentrations of fasting blood glucose significantly increased, and serum insulin reduced in splenectomy mice treated with STZ at the dose of 80 mg/kg. Moreover, beta cell mass decreased, enrichment factor of nucleosomes of islet apoptotic cells and reactive oxygen species produced in pancreas tissues significantly increased in splenectomy mice treated with STZ at the dose of 80 mg/kg.CONCLUSION: Splenectomy increases the sensitivity of islets to STZ in mice by increasing the production of reactive oxygen species in pancreas.     

毕节地区新造林地鼠兔危害状况调查及防控对策

对啮齿类动物鼠兔害的防治.危害调查、标本制作、种类鉴定、生活习性观察,探索有效控制措施.本文着重从森林环境资源的核算评估鼠兔害对新造林地经济损失.     

Depletion of ascorbic acid derivatives in fish feed by the production process

ABSTRACT:   The depletion of ascorbic acid derivatives in fish feed during the feed processing (extrusion cooking and drying) was studied at five extrusion cooking temperatures and at 85°C for 2 h dryer processing temperature. Three ascorbic acid derivatives were used: L-ascorbyl-2-mono-phosphate Mg (APM), L-ascorbic acid sodium (AAS) and L-ascorbic acid palmitic acid ester (AAP). Samples were collected after drying and ascorbic acid derivatives losses evaluated. APM was found to be quiet stable with an average retention of 88%, but AAS and AAP were unstable and the depletion was very high.