青海八里桥绿萝卜品种提纯复壮技术

为解决八里桥绿萝卜由于长期种植导致部分地区品种退化的问题,通过长期的生产实践,采用原种提纯复壮、良种生产、栽培管理等综合技术,实现了品种的提纯复壮,对维持青海八里桥绿萝卜品种的特殊品质具有重要意义。     

茶农绿色防控技术采用行为影响因素实证研究

利用16个茶叶主产省2 210份茶农调查数据,借助Logit模型分析了影响茶农绿色防控技术采用行为的因素。结果表明,茶农采用绿色防控技术受个人特征、家庭特征、茶园经营特征和组织与技术服务特征4个方面的因素影响,其中组织与技术服务特征因素的影响最大。具体来看,年龄、家庭人口规模和茶园规模对茶农采用绿色防控技术有显著的负向影响;农残限量标准认知、家庭年收入、组织化程度、技术培训和技术交流对茶农采用绿色防控技术有显著的积极影响。了解农残限量标准的茶农采用绿色防控技术的概率约是不了解的2倍;家庭收入每增加1万元,技术采用发生比相对增加2%;加入合作社的茶农采用绿色防控技术的概率是未加入的1.73倍;茶农参加病虫害防治培训次数每增加1次,技术采用发生比提升为原来的150%。受教育程度、打工收入占比、农药价格敏感性等因素在本研究中对茶农绿色防控技术采用的影响不显著。由此,有关政府部门需要通过增强茶农组织化程度,加强农户病虫害防治培训,降低技术使用成本等手段促进农户绿色防控技术的采用,以便实现绿色农业。     

不同柱花草绿肥种质磷含量差异研究

柱花草是世界热区重要的绿肥与牧草兼用的豆科作物。作为豆科绿肥,磷含量是影响绿肥品质的重要指标。本文对134份柱花草绿肥种质的磷含量进行了分析评价,结果表明,供试的134份柱花草绿肥种质有133份种质磷含量在0.3%以下,这些柱花草的磷含量达到四级有机肥磷含量标准,仅有1份柱花草种质的磷含量高于0.3%,其磷含量达到三级有机肥磷含量标准。     

Review on the roles and effects of growing media on plant performance in green roofs in world climates

Growing media (substrate) is a fundamental part of a green roof, providing water, nutrients and support to plants. However, little research has reviewed how it affects plant performances in different climatic regions. This study aims to analyse published research on green roof growing medium across world’s climate zones. Findings are structured according to Köppen–Geiger climate classification, aiming to investigate the prevalence of research conducted in different climate zones. Results from full-scale studies and laboratory or greenhouse experiments were reviewed. The later were included as they provide systematic knowledge on the effect of individual factors on system performances although cannot provide climate specific information. Studies discussed effects of major substrate components and depths on plant survival and establishment using standard test procedures. Results showed that most research in the subject were in temperate (group C climate classification), continental (group D) and dry climates (group B), respectively. Considerable number of investigations was conducted in controlled laboratory or greenhouse environments. Based on the results, future green roof research and guidelines should consider climate specifications of the region in designing growing medium, depths and attribute of green roof substrates in order to ensure enhanced plant performance. Especially, for more fragile but less investigated dry climate, considerations should be made to tackle heat fluctuations and drought stress by enhancing water holding capacity and thermal isolation of the substrate. To move forward, sustainable building solutions as a part of future urban forms, climate-adaptive green roof systems should be included into future research, practice and guidelines.     

马铃薯地上部绿色组织中糖苷生物碱合成调控的研究

为提高马铃薯品种(系)地上部分糖苷生物碱(SGAs)的含量,增强其抗逆性并改良块茎的品质。克隆了马铃薯茄啶鼠李糖基转移酶基因(sgt3)cDNA和1,5–二磷酸核酮糖羧化酶小亚基基因启动子(rbcS P);对sgt3亚细胞定位预测显示,该蛋白不具有叶绿体转运肽、线粒体导肽和分泌信号肽序列,推测可能位于细胞质;将克隆的sgt3 cDNA片段及rbcS重组到pCEPSP载体上,构建了具有草甘膦抗性标记的绿色组织特异表达sgt3基因植物表达载体。通过农杆菌介导法对马铃薯品种‘陇薯3号’和‘夏波蒂’进行转化,共获得了12株抗草甘膦的阳性转基因植株。对转基因植株的目的基因表达水平和SGAs含量分析发现,地上部sgt3基因相对表达量较未转化植株提高1.3~3.0倍,SGAs含量增加20%~37%,而转基因植株的块茎中SGAs含量变化不显著。本研究的结果为进一步培育枝、叶中高SGAs而块茎中低SGAs的马铃薯抗性品种提供了理论依据。     

Identification of resistance to Aspergillus flavus infection in cotton germplasm

Natural resistance in cottonseed to Aspergillus flavus infection has not been explored to date. A green fluorescent protein (GFP) expressing A. flavus strain was used to assess the resistance of seed from 35 cotton varieties from Gossypium arboreum, G. barbadense, and G. hirsutum. Mature cotyledons devoid of seed coat were wounded, inoculated, and assessed for innate resistance to A. flavus infection. Of the initial 35 varieties tested, we observed a range of resistance to infection in representatives of all three species. A subset of 15 representatives was further analyzed. Within this group, G. arboreum cultivar A2 186 and G. hirsutum cultivar SA 1582 were most resistant to fungal infection. The most susceptible cultivar in this group was G. hirsutum SA 1595. The remaining 12 representatives tested in the secondary screen (3 G. arboreum, 3 G. barbadense, and 6 G. hirsutum lines) exhibited intermediate resistance. We did not observe any relationship between species and resistance. Within each species, there was a range of responses to fungal infection. Future studies using this methodology to screen additional diploid and tetraploid cotton lines may enable us to identify naturally resistant germplasm that can be used to develop cotton with enhanced resistance to fungal infection.     

Mapping and validation of the quantitative trait loci for leaf stay‐green‐associated parameters in maize

Functional stay‐green is generally regarded as a desirable trait of varieties in major crops including maize. In this study, we used an F_3:4 recombinant inbred line population with 165 lines from a cross between a stay‐green inbred line (Zheng58) and a model inbred line (B73) using 211 polymorphic simple sequence repeat markers to map quantitative trait loci for three stay‐green‐associated parameters, chlorophyll content, photosystem II photochemical efficiency and stay‐green area, at maturity stage, detected a total of 23 quantitative trait loci (QTL) on nine chromosomes. Single QTL explained 3.7–13.5% of the phenotypic variance. Additionally, we validated some important stay‐green QTL using a heterogeneous inbred family approach and found that the stay‐green‐associated parameters were significantly correlated with the plant yield. This study may contribute to a better insight into the regulatory mechanism behind leaf stay‐green in maize and a novel development of elite maize varieties with delayed leaf senescence through molecular marker‐assisted selection.     

基于人行为影响的住区建筑多主体集成能耗模型

在城市建筑节能研究中,政府常常依赖城市能源与碳排放模型工具制定并评估节能策略。而当前的城市建筑能耗模型受限于开发技术等因素,尚存在诸多不足,例如,考虑人行为对建筑能耗的影响,建立行为、日常活动及与环境相关的综合能耗模型。关于此类模型合理的数学形式、参数选择的方法以及不同行为的模拟方式等问题还尚待进一步研究。采用多主体（Multi-Agent）建模技术,设计集成建筑能耗模型,以住区中的居民作为模型中活动的主体,模拟使用者行为习惯、活动对建筑能耗的影响;采用ISO 13790标准模拟建筑的供热和降温需求,并在集成环境中设计统一的描述框架,以相对独立的环境模拟、行为模拟模块形式进行交互,实现热环境模拟和基于主体活动的设备能耗模拟的有机结合。研究表明,住区居民的用能行为是影响城市建筑能耗密度变化的重要因素,实现对住区主体行为特征的引导是实现城市建筑节能目标的有效途径。     

办公建筑中空调形式对室内外PM2.5浓度相关性的影响

室外PM2.5可通过新风及围护结构缝隙渗透至室内,室外PM2.5较高时尤为明显,结果导致室内空气中的PM2.5浓度上升。为了研究空调形式对室内外PM2.5浓度相关性的影响,在2015年夏季对重庆某办公建筑中采用不同空调形式的室内外PM2.5浓度进行了实测。实测结果发现：集中式空调、分体式空调和非空调房间室内外PM2.5浓度比变化范围分别为0.59～0.76、0.47～0.76、0.71～0.91。室内外PM2.5浓度相关性系数的排序为：集中式空调环境（0.94）> 非空调环境（0.92）> 分体式空调环境（0.77）,研究结果表明,办公建筑的空调形式,对室内外PM2.5浓度的相关性有影响。