柑桔黑斑病的发病特点及检测方法研究进展

柑桔黑斑病(Citrus Black Spot,CBS)是一种真菌性病害,主要为害果实,并产生褐色斑点,严重时蔓延至全果,造成果实腐烂,显著影响果实的品质和外观,降低经济价值,被欧美等国列为重要的检疫性病害,近年来该病在我国一些柑桔产区已上升为主要病害。本文主要从柑桔黑斑病的病原菌、发病特点及检测方法等几个方面进行简要的概述。     

默顿职业兴趣的汇聚与转移理论述评

职业兴趣的汇聚与转移是默顿博士论文的第一部分。从科学发展的外部因素来理解科学是默顿论文的意义所在,他对职业兴趣的汇聚与转移的分析恰好为其找到了研究的突破口。用社会学方法来研究科学的发展,虽有其成功的一面,也有其局限性。     

立式金属油罐容量检定中的几个问题

针对JJG168-87计量规程在实际使用中的缺陷，对罐壁圈板径向偏差测量位置的确定，油罐充满油品后直径增大值的修正等问题，提出了具体的解决方法。     

家蚕血液型脓病发生原因及防治方法

以河南省内乡县家蚕血液型脓病发生原因、防治方法进行分析探讨。     

TEOM1405F型PM2.5颗粒物分析仪校准方法的建立

对美国赛默飞世尔公司TEOM1405F颗粒物分析仪制定合理可行的校准方法,并且根据标准方法对仪器进行检定.校准结果为:环境温度传感器误差小于±2℃,环境气压传感器误差小于1 kPa,采样流量相对误差小于±3％,弹性常数K0＜±2％,绝缘阻抗＞20MΩ,满足校准方法技术要求.根据检定结果,该仪器监测数据准确可靠.     

Calculation Method of Hydraulic Characteristics for Direct-Return Chilled Water System

In order to optimize design and operation, it is necessary to calculate accurately hydraulic characteristics large central air-conditioning systems. Simplified Model can not be applied to hydraulic calculation of large pipe network topological structure for its large calculation error. Taking into account the regulating characteristics of terminal branch temperature regulating valve, a direct-return chilled water system is studied, an accurate mathematical model of pipe network hydraulic characteristics has been established and a computer logic algorithm with virtual flow has been given. A direct-return pipe network with ten AHU branches is used as simulation calculation object, the pipe network supply-return water pressure difference is calculated, and also the temperature regulating valve opening and actual flow of each branch are calculated in the condition of different supply-return water pressure difference. The calculated results accord with the natural characteristics of high pressure difference unbalanced for direct-return pipe network. The calculation method can ensure calculation convergence.     

Quantitative PCR assays to enumerate Rhizobium leguminosarum strains in soil also target non viable cells and overestimate those detected by the plant infection method

The plant infection method is commonly used to estimate the Most Probable Number (MPN) of soil rhizobia. Here, a qPCR method was set-up and validated with newly developed ANU (strain specific) and RHIZ (more general) primers to quantify the specific Rhizobium leguminosarum bv. trifolii ANU843 strain or general R. leguminosarum strains. Detection limits of qPCR protocols in soil were 1.2 × 10⁴ (ANU) and 4.2 × 10³ (RHIZ) cells per g soil. The qPCR assay appears robust and accurate in freshly inoculated soils but overestimated MPN for indigenous soil rhizobia. An incubation experiment showed that qPCR detected added DNA or non viable cells in soils up to 5 months after addition and incubation at 20 °C in moist conditions.     

高酸值米糠油酶法酯化脱酸研究

对脂肪酶Novozym 435催化高酸值米糠油酯化脱酸进行了研究.利用响应面分析法(RSM)优化脱酸条件.根据Box-Benhnken中心组合试验设计原理对试验条件进行优化,在分析各因素显著性及其交互作用的基础上,得出米糠油酯化脱酸最佳工艺参数为:酶质量分数1.1％、甘油添加量0.42 mg、温度56.4℃、反应时间23.2 h.实际测得脱酸后米糠油酸值(KOH)由56 mg/g降为5.04 mg/g,游离脂肪酸酯化率达到91％,与模型预测值基本相符.     

快速提取分离八角茴香油的新方法

将回流提取法、水蒸气蒸馏法、快速提取法对八角枝叶或干果中八角茴香油提取进行了比较,并确定八角枝叶或干果中八角茴香油的最佳提取方法和条件。结果表明,快速提取法比回流提取法、水蒸气蒸馏法提取效率高,快速简便。快速提取法提取八角茴香油的最佳条件为:干果原料与水比为1∶6,提取回流时间为2 h,产品收率达12.78%,且含八角茴香脑90.83%。     

高效液相色谱-串联质谱法同时测定水产品中残留的12种喹诺酮和磺胺类药物

建立水产品中有12种喹诺酮类（QNs）和磺胺类（SAs）药物残留量同时测定的高效液相色谱-串联质谱方法。以氘代试剂做内标,选择乙腈提取,用正己烷脱脂,旋转蒸发浓缩,采用LC—MS／MS选择反应监测（SRM）正离子模式测定进行定性、定量分析。结果表明,12种喹诺酮类（QNs）和磺胺类（SAs）药物的检出限（LOD）为1．0μg／kg,定量限（LOQ）为2．0μg／kg,检测结果的相对标准偏差为3．5％～14．6％（n=6）,加标回收率达到69．5％～121．9％。该方法具有比较高的重现性和选择性,在水产品中喹诺酮类和磺胺类药物的残留测定中具有很好的应用前景。