全文获取类型
收费全文 | 72214篇 |
免费 | 2845篇 |
国内免费 | 7996篇 |
专业分类
林业 | 5547篇 |
农学 | 8354篇 |
基础科学 | 4039篇 |
10948篇 | |
综合类 | 33792篇 |
农作物 | 9500篇 |
水产渔业 | 1298篇 |
畜牧兽医 | 4041篇 |
园艺 | 1743篇 |
植物保护 | 3793篇 |
出版年
2024年 | 456篇 |
2023年 | 1357篇 |
2022年 | 2224篇 |
2021年 | 2592篇 |
2020年 | 2568篇 |
2019年 | 2936篇 |
2018年 | 1992篇 |
2017年 | 3260篇 |
2016年 | 4073篇 |
2015年 | 3161篇 |
2014年 | 4290篇 |
2013年 | 4473篇 |
2012年 | 5762篇 |
2011年 | 5229篇 |
2010年 | 4201篇 |
2009年 | 4063篇 |
2008年 | 3490篇 |
2007年 | 3764篇 |
2006年 | 3327篇 |
2005年 | 2794篇 |
2004年 | 2151篇 |
2003年 | 1939篇 |
2002年 | 1494篇 |
2001年 | 1485篇 |
2000年 | 1315篇 |
1999年 | 1140篇 |
1998年 | 983篇 |
1997年 | 886篇 |
1996年 | 849篇 |
1995年 | 819篇 |
1994年 | 632篇 |
1993年 | 623篇 |
1992年 | 553篇 |
1991年 | 506篇 |
1990年 | 444篇 |
1989年 | 364篇 |
1988年 | 258篇 |
1987年 | 227篇 |
1986年 | 146篇 |
1985年 | 37篇 |
1984年 | 29篇 |
1983年 | 10篇 |
1982年 | 13篇 |
1981年 | 28篇 |
1980年 | 16篇 |
1979年 | 21篇 |
1978年 | 14篇 |
1977年 | 13篇 |
1976年 | 16篇 |
1962年 | 21篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
961.
M.C. Hare D.W. Parry M.D. Baker 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(9):859-866
The distribution of seeds by weight for three lots of winter wheat cv. Avalon infected by Fusarium culmorum and three lots of winter wheat cv. Riband infected by Microdochium nivale was determined. The distribution of infected seeds within each seed lot was then determined by isolating F. culmorum from seeds on moist filter paper and M. nivale from seeds on potato dextrose agar. The distribution of M. nivale infected seeds between seeds of different weight was similar to that of the seed lot as a whole, whereas the distribution of F. culmorum was greater in light seeds than heavy seeds. The percentage germination of infected seeds decreased with seed weight. A similar situation was found with respect to seedling emergence in compost for F. culmorum infected seeds. However, with M. nivale infection, similar numbers of seedlings emerged from both light and heavy infected seeds. Seed treatment with guazatine increased seedling emergence for both light and heavy seed infected by M. nivale. However, seedling emergence from F. culmorum infected seed was poor even following treatment with guazatine. Poor emergence was most evident from light seed. 相似文献
962.
苹果叶片总蛋白提取及其双向电泳分析 总被引:1,自引:0,他引:1
蛋白质提取方法和电泳条件的建立是蛋白质组学研究中双向电泳分析的关键。通过比较不同提取方法,包括酚/SDS抽提、TCA-丙酮沉淀法、甲醇/醋酸铵沉淀法以及改良HB-酚抽提方法。此外,还优化了样品上样量、等电聚焦程序设置等条件。从所得2-D图谱分离效果来看,改良HB-酚抽提方法所得蛋白点数最多,2-D图谱背景清晰、分离效果好,明显优于其他3种方法。300μg上样量可作为苹果叶片总蛋白2-DE分析的理想的上样量,同时优化了等电聚焦程序,最终得到的2-D图谱分离效果较为理想。该研究的开展也为苹果叶片蛋白质组学后续研究奠定了基础。 相似文献
963.
Giuseppe Campanile Fabio Zicarelli Domenico Vecchio Corrado Pacelli Gianluca Neglia Anna Balestrieri Rossella Di Palo Federico Infascelli 《Livestock Science》2008,114(2-3):358-361
The influence of a dietary supplementation with Saccharomyces cerevisiae (SC) during the first and the second phase of lactation on dry matter (DM) intake, organic matter digestibility, milk yield and quality and haematological profile was evaluated in buffalo cows. Lactating buffaloes (n = 190), 118.7 days in milk (DIM), were randomly divided into Group C (control, n = 95) and Group T (fed diet supplemented with 98 billion CFU of S. cerevisiae, n = 95). Eight buffaloes for each group (Groups T1 and C1), 85.4 DIM, were used to study the in vivo digestibility and the haematological profile. No differences were found for DM intake (16.5 kg·day− 1) and haematological profile. The SC supplementation increased milk yield (7.9 ± 0.2 vs. 7.4 ± 0.2; P < 0.01) but did not affect milk fat and protein. SC supplementation increased OM digestibility, mainly, in the first phase of lactation (< 135 days), thus allowing a higher energy availability for milk yield and reduced fat mobilization. 相似文献
964.
Hadi Atashi Mazdak Salavati Jenne De Koster Jim Ehrlich Mark Crowe Geert Opsomer the GplusE consortium Miel Hostens 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(3):292-304
The aim of this study was to identify genomic regions associated with 305-day milk yield and lactation curve parameters on primiparous (n = 9,910) and multiparous (n = 11,158) Holstein cows. The SNP solutions were estimated using a weighted single-step genomic BLUP approach and imputed high-density panel (777k) genotypes. The proportion of genetic variance explained by windows of 50 consecutive SNP (with an average of 165 Kb) was calculated, and regions that accounted for more than 0.50% of the variance were used to search for candidate genes. Estimated heritabilities were 0.37, 0.34, 0.17, 0.12, 0.30 and 0.19, respectively, for 305-day milk yield, peak yield, peak time, ramp, scale and decay for primiparous cows. Genetic correlations of 305-day milk yield with peak yield, peak time, ramp, scale and decay in primiparous cows were 0.99, 0.63, 0.20, 0.97 and −0.52, respectively. The results identified three windows on BTA14 associated with 305-day milk yield and the parameters of lactation curve in primi- and multiparous cows. Previously proposed candidate genes for milk yield supported by this work include GRINA, CYHR1, FOXH1, TONSL, PPP1R16A, ARHGAP39, MAF1, OPLAH and MROH1, whereas newly identified candidate genes are MIR2308, ZNF7, ZNF34, SLURP1, MAFA and KIFC2 (BTA14). The protein lipidation biological process term, which plays a key role in controlling protein localization and function, was identified as the most important term enriched by the identified genes. 相似文献
965.
966.
采用田间试验方法,研究了平衡施肥对川中丘陵干旱区高淀粉甘薯徐薯22和川薯34产量和品质的影响.结果表明,与不施肥处理相比,氮磷钾肥配合施用使徐薯22增产40.2%~56.0%,淀粉含量增加1.5~3.3个百分点;川薯34增产38.7% ~52.7%,淀粉含量增加0.2~6.7个百分点.在高产目标下,徐薯22每生产100... 相似文献
967.
L. M. Raboin A. Ramanantsoanirina J. Dusserre F. Razasolofonanahary D. Tharreau C. Lannou M. Sester 《Plant pathology》2012,61(6):1103-1111
The effect of two‐component rice cultivar mixtures on the control of rice blast disease was studied in three different experiments under rainfed upland conditions in the Madagascar Highlands. The mixtures involved a susceptible cultivar (either susceptible or very susceptible) and a resistant cultivar in different mixture arrangements (random or row mixtures) and with different proportions of the susceptible cultivar (50, 20 and 16·7%), which were compared to the susceptible cultivar grown in a pure stand. The effect of these mixtures on the incidence and severity of leaf and panicle blast was measured weekly, and on yield and yield components at harvest time. The mixture effect was more efficient in reducing disease with a proportion of 16·7% susceptible component than with a proportion of 50%. Blast epidemic was significantly reduced in all three experiments. However, under high blast pressure, there was no reduction in the disease by the end of the epidemic and yields of the susceptible cultivar were almost zero whatever the mixture. In two other experiments performed under lower blast pressure, disease incidence and severity were significantly lower in mixtures, and yields of the susceptible cultivars grown in mixtures were higher than those of their respective pure stands. Cultivar mixtures are a promising strategy that could contribute to a more sustainable cultivation of rice under upland conditions in the context of subsistence agriculture in Madagascar, where all cropping operations are manual. 相似文献
968.
Kavallieratos NG Athanassiou CG Pashalidou FG Andris NS Tomanović Z 《Pest management science》2005,61(7):660-666
Laboratory bioassays were carried out in order to evaluate the effectiveness of two diatomaceous earth (DE) formulations, Insecto and SilicoSec, against adults of Rhyzopertha dominica (F) in eight different grain commodities. The adherence of the two DEs to each grain was also measured. The eight grains tested were wheat, whole barley, peeled barley, oats, rye, triticale, rice and maize. These commodities were treated with the DEs at three rates, 0.75, 1.0 and 1.5 g DE kg(-1) grain. The mortality of R dominica adults was measured after 24 h, 48 h, 7 days and 14 days of exposure in the treated grains at 26 degrees C and 55% RH. After the 14-day mortality count, all adults were removed and the treated grains retained under the same conditions for a further 60 days. The treated grains were subsequently examined for F1 progeny. Significant differences were recorded among the eight grain types as well as between the DE formulations tested. After 14 days of exposure, even at the lowest DE rate for both formulations, adult mortality was high (>90%) in wheat and triticale. In contrast, adult mortality was significantly lower in peeled barley. Increasing the rate improved the efficacy of the DEs significantly in only some of the grains tested. Reproductive capacity in all the treated grains was significantly suppressed when compared with untreated grains. Generally, more F1 individuals of R dominica were noted in the treated peeled barley than in the other commodities. Significant differences in the percentage of DE retention were noted among the eight grains. The highest retention level was noted in rice (>87%) and the lowest in maize (<6%). However, the degree of DE adherence to a given grain was not always indicative of the effectiveness of DE against R dominica. 相似文献
969.
不同密度和果穗数对日光温室番茄冠层光合及产量的影响 总被引:2,自引:1,他引:2
以“保红一号”番茄为试材,采用单干整枝方式研究不同密度和果穗数对日光温室番茄功能叶片冠层光合速率、叶绿素含量及产量的影响.结果表明:番茄净光合速率、叶绿素含量在植株上、中、下部冠层间随不同密度的冠高和叶位差异明显.中、上部净光合速率、叶绿素含量高,下部低,3 000株/667m2(留5穗果)各冠层叶位间叶绿素含量与净光合速率呈正相关,表现出冠层净光合速率、叶绿素含量和产量最高.表明适合阿克苏地区冬春茬日光温室番茄生产的适宜密度为3 000株/667m2(留5穗果),总产量最高,达到7 806.3 kg/667m2. 相似文献
970.