除湿降温系统用于温室降温的可行性探索

结合我国温室降温的现状，指出了高温高湿气候条件下直接蒸发降温方法的局限性；提出了利用除湿降温系统来解决高温高湿地区温室降温难的问题；分析了该系统的构成以及实现降温的可行性。     

油茶芽苗砧嫁接的技术环节

从生产实际出发，评述了油茶芽苗砧嫁接技术在圃地选择，选种催芽，荫棚架设，接穗选取，嫁接，栽植，栽后管理等技术环节的应用及注意事项。     

A SNaPshot assay for the rapid and simple detection of known point mutations conferring resistance to ACCase‐inhibiting herbicides in Lolium spp.

Evolution of resistance to herbicides in weeds is becoming an increasing problem worldwide. To develop effective strategies for weed control, a thorough knowledge of the basis of resistance is required. Although non‐target‐site‐based resistance is widespread, target site resistance, often caused by a single nucleotide change in the gene encoding the target enzyme, is also a common factor affecting the efficacies of key herbicides. Therefore, fast and relatively simple high‐throughput screening methods to detect target site resistance mutations will represent important tools for monitoring the distribution and evolution of resistant alleles within weed populations. Here, we present a simple and quick method that can be used to simultaneously screen for up to 10 mutations from several target site resistance‐associated codons in a single reaction. As a proof of concept, this SNaPshot multiplex method was successfully applied to the genotyping of nine variable nucleotide positions in the CT domain of the chloroplastic ACCase gene from Lolium multiflorum plants from 54 populations. A total of 10 nucleotide substitutions at seven of these nine positions (namely codons 1781, 1999, 2027, 2041 2078, 2088 and 2096) are known to confer resistance to ACCase‐inhibiting herbicides. This assay has several advantages when compared with other methods currently in use in weed science. It can discriminate between different nucleotide changes at a single locus, as well as screening for SNPs from different target sites by pooling multiple PCR products within a single reaction. The method is scalable, allowing reactions to be carried out in either 96‐ or 384‐well plate formats, thus reducing work time and cost.     

河南省小麦根、茎部内生真菌多样性及平板拮抗活性研究

To investigate the community composition and diversity of fungal endophytes in wheat roots and stems from different soil types in Henan Province, tissue isolation method was used to isolate and purify endophytic fungi from wheat plants collected from 22 counties/districts in five soil types, and the identification of the fungal endophytes fungi was based on morphology and internal transcribed spacer (ITS) sequence. The community composition and diversity of endophytic fungi were analyzed. The results showed that 426 endophytic fungi were affiliated with 23 genera, among which Chaetomium spp. had the highest number accounting for 23.2% of the total strain number, followed by Penicillium spp.. Five strains were tested for the antifungal activity against both Rhizoctonia Cerealis and Fusarium pseudograminearums on plate, and their inhibition rates against R. Cerealis ranged from 44.8% to 70.5% (zone widths 2.00~9.67 mm)while from 35.4% to 49.3% (zone widths 3.67~6.67 mm) against F. pseudograminearum. This paper clarifies the major taxa and their diversity of fungal endophytes in the roots and stems of wheat in different soil types in Henan Province, and their inhibitory effects on soil-borne fungi, providing a basis for the biological control of sharp eyespot and crown rot of wheat.     

梨小食心虫迷向丝防治桃树梨小食心虫迷向技术探究

为明确迷向丝对梨小食心虫的防治效果,为生产中的合理使用提供理论依据,选用迷向丝在江苏省张家港桃园进行迷向防治试验。结果表明,迷向丝对梨小食心虫有明显的迷向和防治效果,迷向区和迷向+常规农药防治区对比常规用药对照区,迷向效果分别达到了99.79%和99.95%。从今年的示范结果看,迷向丝示范区内,整个试验过程中未用针对梨小食心虫的防治药剂,也控制了其危害程度,没有造成大面积成灾的现象。     

黄河大柳树灌区土壤类型特征与合理开发途径

该文分析了黄河大柳树灌区土壤类型形成条件与分布格局和主要土壤类型的基本特征,在此基础上结合发展灌溉农业对土壤条件的要求,提出土壤资源合理利用与发展灌溉农业的有关问题。     

吊脚楼里的传统土家族家具研究

在分析吊脚楼的起源、发展、历史文化背景的前提下,用中国传统家具的分类方法对土家族家具进行分类;在选材、装饰手法和造型特色等方面与其他传统家具比较异同,揭示了土家族家具的民族特色。     

基于孝感市水禽养殖农户发展前景的分析和研究

文章从对湖北孝感市水禽产业调查入手,结合孝感实地调查报告数据,探讨了孝感市水禽养殖农户发展前景的现状与问题,并提出建议。研究结论对促进相关模式的水禽养殖农户的发展具有一定的借鉴意义。     

草地螟滞育和非滞育幼虫抗寒能力的研究

对草地螟幼虫过冷却点和冰点测定的结果表明,光照、温度、幼虫滞育的深度以及虫体含水量均可以影响草地螟幼虫的抗寒能力。长光照(16 h)条件下发育成的非滞育幼虫过冷却点、冰点均显著低于短光照(10 h)发育而成的滞育幼虫;无论是非滞育幼虫还是滞育幼虫,经低温处理后抗寒能力都会上升。经4℃处理1 d的非滞育幼虫冰点为-2.28℃,而处理25 d的则降低到-4.76℃,过冷却点可从-13.97℃降低到-21.47℃;经4℃处理25、35 d的滞育幼虫,其过冷却点分别为-25.82、-26.79℃,冰点分别为-5.59-、9.13℃。滞育幼虫的过冷却点、冰点与其体重不相关,但与其体内的含水量显著相关。     

丘陵-平原-湿地复合区降雨径流数值模型

针对丘陵-平原-湿地复合地区降雨径流数值模型较少、而已构建的模型对水文要素空间分布和湿地区水流运动又反映不足,采用格子玻尔兹曼法和有限元法构建丘陵-平原-湿地复合区降雨径流数值模型。在模型构建过程中将复合区分为地势起伏较大的上游丘陵区和下游地势变化较小的平原湿地区。上游丘陵区降雨采用距离平方倒数法计算降雨空间分布、采用格子玻尔兹曼法建立非饱和区土壤水运动模型、利用达西公式建立饱和区水流运动模型、利用格子玻尔兹曼法构建汇流数值模型,进而构建了上游区的降雨径流数值模型。平原湿地区采用基于有限元四边形单元构建了水流运动数值模型。平原湿地区入口边界采用上游区降雨径流数值输出的流量过程,平原湿地区出口边界采用基于格子玻尔兹曼法的汇流模型计算的水位过程。四边形单元有限元求解水流运动方程实现了上、下游区的无缝连接,模型使用比较方便。以挠力河流域为研究对象进行了降雨径流和平坦区水流运动模拟研究,模拟结果良好,说明模型较为可靠。