大通中高位山旱地区不同配置模式退耕还林地物种多样性研究

对大通县退耕还林中高位山旱地不同配置模式群落物种多样性进行研究。结果表明:不同配置模式的群落物种多样性指标的变化规律明显不同,多样性指数排序为模式H(白桦 青海云杉)>模式J(青杨 中国沙棘)>模式G(华北落叶松)>模式F(青海云杉)>模式K(青海云杉 中国沙棘)。     

Marine Cyclic Peptides: Antimicrobial Activity and Synthetic Strategies

Oceans are a rich source of structurally unique bioactive compounds from the perspective of potential therapeutic agents. Marine peptides are a particularly interesting group of secondary metabolites because of their chemistry and wide range of biological activities. Among them, cyclic peptides exhibit a broad spectrum of antimicrobial activities, including against bacteria, protozoa, fungi, and viruses. Moreover, there are several examples of marine cyclic peptides revealing interesting antimicrobial activities against numerous drug-resistant bacteria and fungi, making these compounds a very promising resource in the search for novel antimicrobial agents to revert multidrug-resistance. This review summarizes 174 marine cyclic peptides with antibacterial, antifungal, antiparasitic, or antiviral properties. These natural products were categorized according to their sources—sponges, mollusks, crustaceans, crabs, marine bacteria, and fungi—and chemical structure—cyclic peptides and depsipeptides. The antimicrobial activities, including against drug-resistant microorganisms, unusual structural characteristics, and hits more advanced in (pre)clinical studies, are highlighted. Nocathiacins I–III (91–93), unnarmicins A (114) and C (115), sclerotides A (160) and B (161), and plitidepsin (174) can be highlighted considering not only their high antimicrobial potency in vitro, but also for their promising in vivo results. Marine cyclic peptides are also interesting models for molecular modifications and/or total synthesis to obtain more potent compounds, with improved properties and in higher quantity. Solid-phase Fmoc- and Boc-protection chemistry is the major synthetic strategy to obtain marine cyclic peptides with antimicrobial properties, and key examples are presented guiding microbiologist and medicinal chemists to the discovery of new antimicrobial drug candidates from marine sources.     

灌浆初期高温胁迫对水稻籽粒活性氧积累及产量形成的影响

【目的】研究灌浆初期高温胁迫对水稻籽粒活性氧积累及产量形成的影响,为耐热水稻种质资源的筛选和鉴定提供理论依据。【方法】以长江中下游地区普遍推广种植的8个水稻品种为材料,在灌浆初期进行高温胁迫（38 ℃/32 ℃,昼/夜）,研究各水稻品种在灌浆初期高温胁迫下的ROS含量、抗氧化酶活性、淀粉合成相关酶活性、产量及其构成要素以及稻米品质的差异。【结果】与对照相比,灌浆初期高温胁迫导致水稻籽粒O^-₂·和H₂O₂含量及抗氧化酶活性显著增加。在不同水稻品种中,淀粉合成相关酶活性对灌浆初期高温胁迫的响应具有差异性。高温胁迫下,产量损失较大水稻品种的O^-₂·和H₂O₂含量增幅较大,淀粉合成酶活性受高温胁迫影响也较大。灌浆初期高温胁迫导致水稻结实率、千粒质量、收获指数和产量显著下降,其中黄华占和湘两优900的产量损失较小,在灌浆初期的耐热性较强;粤王丝苗、泰优390和湘两优2号的产量损失较大,在灌浆初期的耐热性弱。灌浆初期高温胁迫导致糙米率、精米率和淀粉含量显著下降,垩白粒率和垩白度增加。高温胁迫下,产量损失较大水稻品种的淀粉含量下降幅度较大,稻米品质受高温胁迫影响也较大。在高温胁迫下,水稻产量与ROS含量显著负相关,而与抗氧化酶活性显著正相关。产量、籽粒加工指标、淀粉含量和淀粉合成酶活性的抗逆系数与ROS含量的抗逆系数显著负相关,而与抗氧化酶活性的抗逆系数显著正相关。【结论】灌浆初期高温胁迫导致水稻籽粒ROS过量积累是降低淀粉合成酶活性和淀粉含量,进而导致产量和稻米外观加工品质下降的重要因素。ROS的增加量可作为灌浆初期水稻耐热性评价的参考指标。     

农产品冷链物流高质量发展水平评价及障碍度分析

推动冷链物流高质量发展,对减少农产品产后损失、扩大消费品优质供给、保障食品安全有重要作用。基于我国2012-2021年统计数据,构建了包括经济基础、物流发展、冷链运营、开放创新、绿色协调5个一级指标及21个二级指标的农产品冷链物流高质量发展评价指标体系,利用熵值法确定各指标的权重,综合评价指数确定得分水平,借助障碍度模型分析发展障碍因子。结果显示：我国农产品冷链物流高质量发展水平总体呈上升趋势且增速较快,到2021年水平值达到0.85,是初始状态的近13倍,其中开放创新水平的贡献度最高;阻碍高质量发展的障碍因素在2017年之前为开放创新水平,之后则是绿色协调水平。为进一步提高我国农产品冷链物流高质量发展水平,需要充分利用国内外两个市场、促进产业协同、发展绿色低碳物流。     

检疫性杂草皱匕果芥的研究进展

从检疫性杂草皱匕果芥对除草剂抗性的现状和机制,影响种子萌发和出苗的因素,出苗时间对生长和繁殖的影响,潜在利用和控制策略等方面介绍了国内外对皱匕果芥的研究进展,指出了目前我国皱匕果芥检疫管制措施存在的问题,提出目前应从加强疫情监测、增补列入进境检疫名录、加强检疫监管和防控宣教等方面做好皱匕果芥的综合防控。     

转Bt基因棉种植前后3个棉铃虫种群对4种杀虫剂的抗性变化

分别于1990年、1992年、2001年和2005年从山东泰安、山东夏津和湖北天门采集3个棉铃虫H elicoverpa arm igera种群,采用点滴法测定了4种杀虫剂对其的毒力,以监测各种群的抗药性变化趋势。结果表明:19901992年3个种群棉铃虫对氰戊菊酯的抗性增长迅速(13.256.1倍);19922001年抗性增长速度有所减缓(3.111.0倍);20012005年抗性水平降低。3个种群棉铃虫对灭多威和辛硫磷的抗性变化趋势与氰戊菊酯的基本相似,但抗性水平较低;对硫丹则均一直处于敏感水平。引起抗药性变化的原因主要与各类药剂的使用频率和转B t基因抗虫棉的大面积推广有关。     

天山地形对新疆大风和降温天气作用的数值模拟研究

2009年5月24日夜间～26日夜间,新疆地区自西向东出现了明显的降水、大风、降温天气过程。为研究此次天气过程中天山地形的作用,文中用WRFV3.1模式对其进行了数值模拟,并通过改变天山山脉的地形高度设计了一组敏感性试验,分析了天山地形对此次强天气过程中大风和降温的影响。结果表明,天山山脉地形对准格尔盆地西北部直至天山山脉北侧的气流有明显的阻挡与减缓作用,对三十里风区、百里风区、汗腾格里峰北坡强风带的形成有直接而重要的作用;地形高度越高,产生垂直运动的地形抬升机制越强,地形强迫抬升作用产生的垂直上升运动越剧烈;天山山脉明显地减轻了寒潮天气过程中南疆塔里木盆地的降温幅度,对准格尔盆地北部的寒潮降温幅度也有一定的减缓作用,对塔里木盆地的"保温"作用较准格尔盆地明显。     

病原菌诱导后黄瓜叶片中脂氧合酶活性与茉莉酸积累的关系

通过酶活性测定和高效液相色谱分析,研究经病原菌诱导后黄瓜叶片中脂氧合酶(LOX)活性与茉莉酸(JA)积累量的关系。结果表明,黄瓜经褐斑菌诱导后,叶片中茉莉酸(JA)大量积累,同时脂氧合酶(LOX)活性上升,接种叶片的上位叶中LOX活性和JA含量于诱导接种褐斑菌12h后开始升高,并于诱导接种24h后达到峰值,接种叶片的下位叶中LOX活性和JA含量在诱导接种12h后变化不大,但在24h后显著升高,并于48h达到峰值,此后逐渐平稳下降。相关分析表明,LOX与JA呈显著正相关,相关系数r为0.92。同时通过喷施脂氧合酶抑制剂(NDGA)和添加茉莉酸的试验证实LOX是JA合成的关键酶。     

Effects of mutations in Drosophila nicotinic acetylcholine receptor subunits on sensitivity to insecticides targeting nicotinic acetylcholine receptors

Several strains of Drosophila melanogaster possess mutant alleles in nicotinic acetylcholine receptor (nAChR) subunits, Dα1 and Dβ2 that confer resistance to neonicotinoids such as imidacloprid and nitenpyram, and Dα6, that confers resistance to spinosyns. These mutant strains were bioassayed with a selected set of nAChR active insecticides including neonicotinoids, spinosad, and sulfoxaflor, a new sulfoximine insecticide. All of the neonicotinoids examined, except dinotefuran showed reduced insecticidal efficacy on larvae of the Dα1 mutant, suggesting that this subunit may be important in the action of these insecticides. All of the neonicotinoids, including dinotefuran, showed reduced insecticidal efficacy on larvae possessing the Dβ2 mutation. A similar pattern of broad neonicotinoid resistance to that of Dβ2 alone was also observed for larvae with both the mutations (Dα1 + Dβ2). The Dβ2 mutation exhibited a lower level of cross-resistance to sulfoxaflor (<3-fold) than to any of the neonicotinoids (>13-fold). In contrast, there was no cross-resistance for any of the neonicotinoids or sulfoxaflor in adult flies with the Dα6 mutation, which confers high levels of resistance to spinosad. Thus in the D. melanogaster strains studied, target site resistance observed for the neonicotinoids and the spinosyns does not translate directly to resistance towards sulfoxaflor.     

Problems of implementing compulsory integrated pest management

Compulsory integrated pest management poses a number of challenges and issues for debate. Some of are discussed with reference to European Union requirements set out in Annex III of Directive 128/2009. Requirements on the use of plant protection products bring with them problems with dose reduction in the light of a resistance prevention strategy, the lack of threshold levels, the necessity of chemical treatments and the limited availability of effective products of biological origin. Use of preventive measures of pest control faces barriers related to profitability and a lack of knowledge on the part of farmers. Challenges to control the system, as well as the fact that some legally enforced rules are not suitable for all crops, seem also to be an issue. © 2019 Society of Chemical Industry