PCR-RFLP技术检测新嘉兴黑猪氟烷基因

本试验随机抽取嘉兴地区部分猪场新嘉兴黑猪血样,通过PCR-RFLP技术进行氟烷基因检测,结果表明,37头新嘉兴黑猪基因型均为NN,未出现Nn和nn基因型。检测结果为新嘉兴黑猪品种资源保护及合理开发利用提供理论依据。     

梅花鹿生长激素基因的克隆及序列分析

对梅花鹿的生长激素基因I部分片段进行了克隆测序 ,序列长度为 14 6 6bp ,现已将该序列提交到GeneBank上。GeneBank中的Blast分析表明 ,梅花鹿生长激素基因I与马鹿同源性为 99% ,与鼷鹿同源性为84 %。     

鸡肠炎沙门氏菌外膜蛋白F的原核表达及免疫原性分析

根据GenBank中收录的鸡肠炎沙门氏菌OmpF基因序列设计1对引物,以鸡肠炎沙门氏菌辽宁分离株基因组为模板,扩增鸡肠炎沙门氏菌OmpF基因,用IEDB Analysis Resource在线预测分析,该基因可能存在15个线性B细胞抗原表位;同时将该基因克隆至原核表达载体PGEX-4T-1中,构建重组表达质粒PGEX-4T-1-OmpF,转入大肠杆菌BL21中,获得OmpF重组蛋白。Western-blot检测结果表明,该重组蛋白的分子质量约为66 kD(含标签蛋白),能被鸡沙门氏菌阳性血清识别,初步证实该蛋白具有免疫原性,为鸡肠炎沙门氏菌亚单位疫苗的进一步研究和开发奠定了理论基础。     

淮南猪酸肉基因的多态性分析

为了研究淮南猪的种质特性,为淮南猪保种、可持续利用及种质创新提供依据,试验采用PCR-RFLP法,对96头淮南猪的RN基因进行了检测。结果表明,RN基因在淮南猪群体中存在隐性纯合子rn^+/rn^+和杂合子RN^-/rn^+,未发现显性纯合子RN^-/RN^-。其中,基因型rn^+/rn^+和RN^-/rn^+的频率分别为0.739 6和0.260 4;等位基因rn^+和RN^-的频率分别为0.869 8和0.130 2。卡方检验表明,rn^+和RN^-基因频率处于Hardy-Weinberg平衡状态,表明群体中RN基因没有受到选择的压力。     

绵羊BMPR-IB基因作为多胎性能的候选基因在肉用多胎品种选育中的应用研究

以中国美利奴多胎细毛羊和南非肉用细毛羊为亲本，以BMPR-IB基因作为多胎性能的候选基因，进行多胎肉用品种的选育。通过对其后代多胎基因型检测分析表明：多胎这一性状的选择，符合孟德尔遗传规律，从而验证其多胎性状由一对多胎基因(FecB)控制。通过BMPR-IB基因分子标记辅助选择，多胎性状的选择仅需3个世代(3～6年)的时间就可以达到要求，对于肉用这一性状的选择，还有待于进一步选配选育。     

乳铁蛋白的生物学功能及其基因工程研究进展

乳铁蛋白是一种具有多种生物活性的糖蛋白。本文主要就乳铁蛋白的生物学功能及其基因工程研究进展作一综述。     

3株鸽源新城疫病毒的分子特征及对鸽的致病性

2011-2013年在新城疫流行病学调查中分离到3株鸽源新城疫病毒(SDS,SD01和SD02),为了进一步了解其生物学特性和遗传进化规律,对3株病毒进行了测序和生物活性分析,并对分离株SDS对鸽的致病性进行了评价.结果表明,毒株SDS基因组全长为15 192 bp,基因排列方式为3'-NP-P-M-F-HN-L-5'...     

Laminar inflammatory gene expression in the carbohydrate overload model of equine laminitis

Reasons for performing study: There is a need to assess the laminar inflammatory response in a laminitis model that more closely resembles clinical cases of sepsis‐related laminitis than the black walnut extract (BWE) model. Objectives: To determine if a similar pattern of laminar inflammation, characterised by proinflammatory cytokine expression, occurs in the CHO model of laminitis as has been previously reported for the BWE model. Methods: Sixteen horses administered 17.6 g of starch (85% corn starch/15% wood flour)/kg bwt via nasogastric (NG) tube were anaesthetised either after developing a temperature >38.9°C (DEV group, n = 8) or at onset of Obel grade 1 lameness (OG1 group, n = 8). Control horses (CON group, n = 8) were anaesthetised 24 h after NG administration of 6 l of deionised water. Laminar tissue was collected from horses while under anaesthesia, followed by humane euthanasia. Real time‐quantitative PCR was used to assess laminar mRNA concentrations of genes involved in inflammatory signalling. Results: Increased mRNA concentrations (P<0.05) for IL‐1β, IL‐6, IL‐12p35, COX‐2, E‐selectin and ICAM‐1 were present in laminae from horses with OG1 lameness but not at the DEV time, when compared to the CON horses. No differences between the groups were found for IL‐2, IL‐4, IL‐10, TNF‐α, IFN‐γ or COX‐1 at either the DEV or OG1 time points. Conclusions: There was a notable difference in the temporal pattern of inflammatory events between the BWE and CHO models, with the majority of laminar inflammatory events appearing to occur at or near the onset of lameness in the CHO model, whereas many of these events peak earlier in the developmental stages in the BWE model. This suggests that, in addition to circulating inflammatory molecules, there may be a local phenomenon in the CHO model resulting in the simultaneous onset of multiple laminar events including endothelial activation, leucocyte emigration and proinflammatory cytokine expression. Potential relevance: The similar (although somewhat delayed) inflammatory response in the CHO model of laminitis indicates that inflammatory signalling is a consistent entity in the pathophysiology of laminitis.     

枇杷LEAFY同源基因的克隆及序列分析

为从分子水平研究枇杷成花的机理,通过分析植物花分生组织决定基因LEAFY(LFY)同源基因的保守区序列,设计了1对简并引物,用PCR方法从枇杷栽培品种‘早钟6号’基因组DNA中扩增出1个1317bp的片段,把该片段克隆到pUCm-T载体,测序和序列分析结果表明获得了枇杷LFY同源基因(ejLFY)3’端的1个片段,该片段有1个911bp的内含子,编码区共编码130个氨基酸,其序列已经在GenBank中登记(登录号为AY551183)．在GenBank中进行同源性搜索,发现该基因片段与其他作物中已经报道的LFY同源基因的同源性大都在94％以上,特别是与同属于蔷薇科的苹果的同源性最高,达到98％的同源性,推测它们具有相似的功能．