The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte

The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP⁺ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell.     

上海松江中心城区道路绿化类型及景观分析

将松江中心城区道路绿化的配置形式归纳为14个类型,并对各类型的绿化景观效果进行了分析。     

北方农牧交错带土地利用结构的线性规划——以定西县为例

探讨了用线性规划方法优化土地利用结构,并以北方农牧交错带定西县土地利用结构的经济效益作为目标函数,以人口和自然条件作为约束条件,进行优化分析,规划结果为定西县土地利用结构由2005年的农、林、草面积比例54:10:36调整为30:13:57.     

Reuolution and Research of Green Automobile

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.     

Land Storage System: Effect, Problem and Its Legal Regulation

The authors suggest that the land storage system is beneficial not only to strengthen the country's macroscopic regulation of city land market, accelerate healthy development of city economy and increase the financial income, it can also be advantageous to carry out the economic reform and public facilities construction without a hitch. On the basis of this, in this paper, the increment of the land price and house price as well as the high risk of fund due to lack of related laws is analyzed. Corresponding legal regulations, such as polishing the legislation of land right, implementing the land utilization planning, reinforcing the legislation of city development, land requisition and countryside residential land administration are put forward.     

Study on the Land Use Right in Urban Housing Dismantling and Moving

With the strengthening of constitutional protection that legal private property is not to be encroached upon and the land usufructs paid regime are further clear, the regime of urban housing dismantling and moving is facing a vigorous test. This paper analyzes three problems: the justice of the withdrawal of land use right, the legality of the alteration of the land use right and the rationality of the compensation of the land use right during the urban housing dismantling and moving. Finally, this paper tries to find some effective measures to solve the chain problems.     

Exploring Harmonious Protection of Land Resources in City Construction

Urbanization in China is at an essential point in its transformation from the initial stage to accelerating stage. With quick expansion of city scale and large occupation of land for urban construction, the contradiction between land resource shortage and people's living needs becomes more and more prominent. It has great affection on the grand plan of China's sustainable development of economy and society. How to adjust the relations between the development of urban construction and protect the land resource, promote the rational use of urban land, perfect and complete the protection system of land resource for improving the efficiency of urban land use. In order to conserve land resource and find a sustainable way for development of people's living and environment construction, the above mentioned problems are the most important issue for urban construction and important subject of this paper.     

山区庄园式开发模式研究——以河北省元氏县为例

本文对河北省元氏县山区庄园式开发模式进行了论述,该模式通过山区“四荒”(荒山、荒坡、荒沟、荒滩)地使用权的拍卖调动了群众治理荒山的积极性,将“庄”与“园”相结合,对山区进行综合性、立体化治理,实现了生态效益、经济效益和社会效益的协调发展,是实现山区农民脱贫致富的有效途径。     

人民公社阶段的农地产权制度变迁及其绩效

研究人民公社阶段的农地产权制度变迁与绩效，是为了分析人民公社制度得以长期维持的原因，以期为新时期农村经济体制改革提供启示与借鉴。文章在相关文献资料的基础上，采用实证描述与规范研究分析方法，探讨人民公社阶段的农地产权制度的演进、特征、变迁的原因、内在缺陷以及制度绩效，研究表明，人民公社阶段农地产权严重残缺、缺少激励机制、组织管理费用高昂，这种制度安排实际上一开始就处于非均衡状态，依靠国家主流的意识形态和超经济强制来维持，致使人民公社阶段农地产权制度效率低下。改革开放后，中国社会政治和意识形态方面的约束放松，获取存在于人民公社制度之外的巨大外部利润，推动了农地产权制度创新。