首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   121篇
  免费   13篇
  国内免费   10篇
林业   5篇
农学   3篇
  4篇
综合类   28篇
农作物   6篇
水产渔业   7篇
畜牧兽医   55篇
园艺   9篇
植物保护   27篇
  2024年   1篇
  2023年   1篇
  2022年   3篇
  2021年   3篇
  2020年   4篇
  2019年   3篇
  2018年   8篇
  2017年   9篇
  2016年   5篇
  2015年   3篇
  2014年   5篇
  2013年   11篇
  2012年   10篇
  2011年   12篇
  2010年   4篇
  2009年   7篇
  2008年   11篇
  2007年   9篇
  2006年   4篇
  2005年   1篇
  2004年   4篇
  2003年   3篇
  2002年   2篇
  2001年   3篇
  2000年   3篇
  1999年   6篇
  1998年   1篇
  1997年   2篇
  1994年   1篇
  1993年   2篇
  1989年   3篇
排序方式: 共有144条查询结果,搜索用时 15 毫秒
91.
92.
93.
利用重组人白细胞介素1受体拮抗药(IL 1ra),一次性给小鼠雾化吸入IL 1ra1.0、3.0、10.0mg·kg-1和等容量的磷酸缓冲液(PBS),研究其对小鼠中枢神经系统的药理作用。结果表明:IL 1ra(3.0mg·kg-1)在给药后20min内可引起小鼠自主活动次数的明显增多;并且IL 1ra呈剂量依赖性地对抗戊巴比妥钠的中枢抑制作用,使小鼠睡眠潜伏期延长、持续时间缩短,与PBS对照组相比差异显著或极显著,说明IL 1ra对小鼠中枢神经系统可产生兴奋作用。  相似文献   
94.
ObjectiveTo describe the effects of alpha2-adrenergic receptor antagonists on the pharmacodynamics of sublingual (SL) detomidine in the horse.Study designRandomized crossover design.AnimalsNine healthy adult horses with an average age of 7.6 ± 6.5 years.MethodsFour treatment groups were studied: 1) 0.04 mg kg?1 detomidine SL; 2) 0.04 mg kg?1 detomidine SL followed 1 hour later by 0.075 mg kg?1 yohimbine intravenously (IV); 3) 0.04 mg kg?1 detomidine SL followed 1 hour later by 4 mg kg?1 tolazoline IV; and 4) 0.04 mg kg?1 detomidine SL followed 1 hour later by 0.12 mg kg?1 atipamezole IV. Each horse received all treatments with a minimum of 1 week between treatments. Blood samples were obtained and plasma analyzed for yohimbine, atipamezole and tolazoline concentrations by liquid chromatography-mass spectrometry. Behavioral effects, heart rate and rhythm, glucose, packed cell volume (PCV) and plasma proteins were monitored.ResultsChin-to-ground distance increased following administration of the antagonists, however, this effect was transient, with a return to pre-reversal values as early as 1 hour. Detomidine induced bradycardia and increased incidence of atrioventricular blocks were either transiently or incompletely antagonized by all antagonists. PCV and glucose concentrations increased with tolazoline administration, and atipamezole subjectively increased urination frequency but not volume.Conclusions and clinical relevanceAt the doses administered in this study, the alpha2-adrenergic antagonistic effects of tolazoline, yohimbine and atipamezole on cardiac and behavioral effects elicited by SL administration of detomidine are transient and incomplete.  相似文献   
95.
Phlorotannins are polyphenolic compounds in marine alga, especially the brown algae. Among numerous phlorotannins, dieckol and phlorofucofuroeckol-A (PFF-A) are the major ones and despite a wider biological activity profile, knowledge of the G protein-coupled receptor (GPCR) targets of these phlorotannins is lacking. This study explores prime GPCR targets of the two phlorotannins. In silico proteocheminformatics modeling predicted twenty major protein targets and in vitro functional assays showed a good agonist effect at the α2C adrenergic receptor (α2CAR) and an antagonist effect at the adenosine 2A receptor (A2AR), δ-opioid receptor (δ-OPR), glucagon-like peptide-1 receptor (GLP-1R), and 5-hydroxytryptamine 1A receptor (5-TH1AR) of both phlorotannins. Besides, dieckol showed an antagonist effect at the vasopressin 1A receptor (V1AR) and PFF-A showed a promising agonist effect at the cannabinoid 1 receptor and an antagonist effect at V1AR. In silico molecular docking simulation enabled us to investigate and identify distinct binding features of these phlorotannins to the target proteins. The docking results suggested that dieckol and PFF-A bind to the crystal structures of the proteins with good affinity involving key interacting amino acid residues comparable to reference ligands. Overall, the present study suggests α2CAR, A2AR, δ-OPR, GLP-1R, 5-TH1AR, CB1R, and V1AR as prime receptor targets of dieckol and PFF-A.  相似文献   
96.
[目的]为拮抗菌的商业化贮藏应用提供依据。[方法]通过室内培养筛选出2株具有拮抗效果的酵母菌,通过活体和离体试验,研究拮抗酵母菌对青霉引起的苹果采后病害的防治效果。[结果]活体条件下,含量为2×108CFU/ml的2株酵母菌对青霉菌具有较明显的抑制效果,其中菌株1的防效为89%,菌株2的防效为92%;离体条件下,含量为106CFU/ml的2株酵母菌可完全抑制青霉菌生长;用酵母菌菌悬液浸果后,贮藏20 d时苹果未出现腐烂现象,20 d后酵母菌菌株2在苹果上的数量为初始值的15.0倍,菌株1在苹果上的数量为初始值的10.2倍。[结论]2株酵母菌均可在苹果伤口上迅速繁殖,可有效防治苹果青霉病。  相似文献   
97.
Eight free-ranging axis deer (Axis axis) were captured in drive nets and injected with xylazine (3.4±0.1 mg/kg; mean ±SEM) intramuscularly using a hand-held syringe. Xylazine induced complete immobilization and sedation in three animals, heavy sedation in three, and moderate sedation in two. The mean induction time was 10.4±1.0 min. The mean rectal temperature, heart and respiratory rates of immobilized animals were 39.2±0.4°C, 75.5±6.5 beats/min and 62.1±4.2 breaths/min, respectively.All the animals were given atipamezole intravenously for reversal. The mean time from injection of xylazine to administration of atipamezole was 37.8±4.6 min. A dose ratio (w/w) for xylazine:atipamezole-HCl of 10:1 was used. The mean time from injection of atipamezole to mobility was 2.41±0.58 min.Atipamezole given intravenously effectively antagonized xylazine-induced sedation in axis deer. Only one animal showed signs of overalertness after reversal and no cases of resedation were observed.Abbreviations i.m. intramuscular(ly) - i.v. intravenous(ly) - SEM standard error of the mean  相似文献   
98.
不同种类生物有机肥对烤烟青枯病及烟叶生长的影响   总被引:1,自引:1,他引:1  
为探索不同种类生物有机肥对烟叶青枯病的防治效果,以期为生态、优质、安全烤烟生产的田间管理提供科学参考。通过田间小区试验,对7种不同有机肥配施拮抗菌对烤烟生长、产量及青枯病的影响效果做了分析比较。结果表明:有机肥配施拮抗菌促进了烟叶前期生长,上等烟比例提高了12.80~33.59个百分点,但产量降低了2.91%~38.18%;7个生物有机肥处理均减缓了烟叶生长前期的青枯病发病情况,进入成熟采烤期后,只有处理4[3750 kg/hm2有机肥(20%菜枯+20%碳化谷壳+60%烟草秸秆粉碎物)]、处理3[3750 kg/hm2有机肥(40%茶枯+60%烟草秸秆粉碎物)]和处理5[3750 kg/hm2有机肥(20%茶枯+20%碳化谷壳+60%烟草秸秆粉碎物)]的病情指数低于对照,防治效果分别为54.22%、43.37%和31.32%。采用处理4、处理3和处理5时,应根据烟叶田间生长情况,适当进行追肥,以达到既能提高烟叶的产量、上等烟比例,又能防治烟叶青枯病的效果。  相似文献   
99.
苏醒灵4号对兔心血管与呼吸系统的影响   总被引:1,自引:0,他引:1  
苏醒灵4号是新型的动物麻醉拮抗剂。利用动脉插管技术探讨了该药对清醒兔和眠乃宁麻醉兔心血管和呼吸系统的影响,结果表明,苏醒灵4号对清醒兔心血管和呼吸系统无明显的影响,但能迅速拮抗眠乃宁对兔心血管和呼吸系统的抑制作用,表现为给药后1minHR、SP、DP、MAP和RR明显回升,3min时SP、DP、MAP和RR值达正常水平(P〉0.05),而HR在30min时恢复至正常水平。眠乃宁对心电有明显的影响,Q-T、P-R和QRS间隙延长,给予苏醒灵4号后min时开始回升,90min恢复至正常水平。  相似文献   
100.
采用组织分离法和稀释平板法对西南桦猝倒病发生区健康苗木组织、根际土壤及咖啡壳堆肥化基质进行菌种分离和分子鉴定。将分离获得的17株菌株与西南桦苗木猝倒病病原菌尖孢镰刀菌和腐皮镰刀菌进行平板对峙实验,筛选病原拮抗菌。结果表明,筛选出的真菌5#、7#及细菌BJ、JCl对西南桦苗木猝倒病原菌尖孢镰刀菌和腐皮镰刀菌具有较强抑制效果。其中真菌5#和7#的抑菌率分别为91.30%和90.12%;细菌BJ和JCl抑菌带较宽,对峙4天分别达0.4cm和0.5cm,抑菌率分别为80.4%和78.6%,且BJ抑菌效果更好;真菌与细菌比较,拈抗真菌5#和7#比拮抗细菌BJ和JCl抑制热区西南桦苗木猝倒病菌效果更好。通过对峙实验筛选出的5#、7#、BJ和JCl拮抗菌,对热区防治苗木猝倒病及生防制剂的开发应用具有参考价值。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号